# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1804764 | B | Binding affinity to rhoA assessed as increase in response unit after 120 mins by SPR assay | 3 | single protein format | Scientific Literature | |||
2. | ALA1805095 | B | Inhibition of rhoA expressed in Escherichia coli BL21 after 1 hr by G-LISA activation based chemiluminescence assay | 9 | assay format | Scientific Literature | |||
3. | ALA1805097 | B | Binding affinity to rhoA by SPR assay | 3 | single protein format | Scientific Literature | |||
4. | ALA1805098 | B | Inhibition of rhoA expressed in Escherichia coli BL21 at 2.5 uM after 1 hr by G-LISA activation based chemiluminescence assay | 41 | assay format | Scientific Literature | |||
5. | ALA1035285 | B | Inhibition of PDGF-BB-stimulated RhoA activity in human aortic smooth muscle cells assessed as reduction in ratio of RhoAGTP/RhoA levels at 25 uM after 4 hrs by pull-down assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
6. | ALA1930874 | B | Inhibition of RhoA activation in human HepG2 cells assessed as decrease of membrane-associated protein level at 16 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
7. | ALA1930875 | B | Inhibition of RhoA activation in human L02 cells assessed as decrease of membrane-associated protein level at 16 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
8. | ALA1931129 | B | Inhibition of RhoA activation in human HepG2 cells assessed as decrease of membrane-associated protein level at 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
9. | ALA2398825 | B | Inhibition of RhoC-mediated pathway in human SK-Mel-147 cells assessed as inhibition of SRE-regulated gene transcription by luciferase reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
10. | ALA2398833 | B | Inhibition of RhoC-mediated pathway in human PC3 cells assessed as inhibition of SRE-regulated gene transcription at 100 uM by luciferase reporter gene assay | Homo sapiens | 53 | cell-based format | Scientific Literature | ||
11. | ALA2398834 | B | Inhibition of RhoC-mediated pathway in human PC3 cells assessed as inhibition of SRE-regulated gene transcription by luciferase reporter gene assay | Homo sapiens | 53 | cell-based format | Scientific Literature | ||
12. | ALA3222927 | B | Inhibition of PDGF-BB-induced RhoA activation in human SMC assessed as intracellular RhoA-GTP level at 25 uM incubated for 4 hrs prior to PDGF-BB stimulation for 2 mins by G-LISA | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
13. | ALA3241051 | B | Activation of RhoA in human smooth muscle cells assessed as PDGF-BB stimulated GTP bound protein level at 10 uM preincubated for 4 hrs followed by PDGF-BB stimulation measured after 2 mins by G-LISA assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
14. | ALA3854048 | B | Activation of RhoA in human diabetic primary keratinocytes at 10 nM after 3 hrs by immunoblot method | Homo sapiens | 2 | assay format | Scientific Literature | ||
15. | ALA4417928 | B | Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 2 uM by Western blotting based pulldown assay (Rvb = 100%) | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
16. | ALA4417929 | B | Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 4 uM by Western blotting based pulldown assay (Rvb = 100%) | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
17. | ALA4417930 | B | Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 5 uM by Western blotting based pulldown assay (Rvb = 100%) | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
18. | ALA4417931 | B | Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 10 uM by Western blotting based pulldown assay (Rvb = 100%) | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
19. | ALA4419720 | B | Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced phospho-MLC formation at 50 uM incubated for 24 hrs by densitometry | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
20. | ALA4419724 | B | Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced focal adhesion complex formation at 50 uM by anti-vinculin antibody staining based assay | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data |