#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1035285	B	Inhibition of PDGF-BB-stimulated RhoA activity in human aortic smooth muscle cells assessed as reduction in ratio of RhoAGTP/RhoA levels at 25 uM after 4 hrs by pull-down assay	Homo sapiens	5	ALA1158458	assay format	Scientific Literature
2.	ALA1804764	B	Binding affinity to rhoA assessed as increase in response unit after 120 mins by SPR assay		3	ALA1799914	single protein format	Scientific Literature
3.	ALA1805095	B	Inhibition of rhoA expressed in Escherichia coli BL21 after 1 hr by G-LISA activation based chemiluminescence assay		9	ALA1799914	assay format	Scientific Literature
4.	ALA1805097	B	Binding affinity to rhoA by SPR assay		3	ALA1799914	single protein format	Scientific Literature
5.	ALA1805098	B	Inhibition of rhoA expressed in Escherichia coli BL21 at 2.5 uM after 1 hr by G-LISA activation based chemiluminescence assay		41	ALA1799914	assay format	Scientific Literature
6.	ALA1930874	B	Inhibition of RhoA activation in human HepG2 cells assessed as decrease of membrane-associated protein level at 16 uM after 24 hrs by Western blot analysis	Homo sapiens	1	ALA1926516	cell-based format	Scientific Literature
7.	ALA1930875	B	Inhibition of RhoA activation in human L02 cells assessed as decrease of membrane-associated protein level at 16 uM after 24 hrs by Western blot analysis	Homo sapiens	1	ALA1926516	cell-based format	Scientific Literature
8.	ALA1931129	B	Inhibition of RhoA activation in human HepG2 cells assessed as decrease of membrane-associated protein level at 10 uM after 24 hrs by Western blot analysis	Homo sapiens	1	ALA1926516	cell-based format	Scientific Literature
9.	ALA2398825	B	Inhibition of RhoC-mediated pathway in human SK-Mel-147 cells assessed as inhibition of SRE-regulated gene transcription by luciferase reporter gene assay	Homo sapiens	1	ALA2396514	cell-based format	Scientific Literature
10.	ALA2398833	B	Inhibition of RhoC-mediated pathway in human PC3 cells assessed as inhibition of SRE-regulated gene transcription at 100 uM by luciferase reporter gene assay	Homo sapiens	53	ALA2396514	cell-based format	Scientific Literature
11.	ALA2398834	B	Inhibition of RhoC-mediated pathway in human PC3 cells assessed as inhibition of SRE-regulated gene transcription by luciferase reporter gene assay	Homo sapiens	53	ALA2396514	cell-based format	Scientific Literature
12.	ALA3222927	B	Inhibition of PDGF-BB-induced RhoA activation in human SMC assessed as intracellular RhoA-GTP level at 25 uM incubated for 4 hrs prior to PDGF-BB stimulation for 2 mins by G-LISA	Homo sapiens	4	ALA3217440	cell-based format	Scientific Literature
13.	ALA3241051	B	Activation of RhoA in human smooth muscle cells assessed as PDGF-BB stimulated GTP bound protein level at 10 uM preincubated for 4 hrs followed by PDGF-BB stimulation measured after 2 mins by G-LISA assay	Homo sapiens	6	ALA3232906	cell-based format	Scientific Literature
14.	ALA3854048	B	Activation of RhoA in human diabetic primary keratinocytes at 10 nM after 3 hrs by immunoblot method	Homo sapiens	2	ALA3853270	assay format	Scientific Literature
15.	ALA4417928	B	Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 2 uM by Western blotting based pulldown assay (Rvb = 100%)	Homo sapiens	1	ALA4417630	cell-based format	Patent Bioactivity Data
16.	ALA4417929	B	Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 4 uM by Western blotting based pulldown assay (Rvb = 100%)	Homo sapiens	1	ALA4417630	cell-based format	Patent Bioactivity Data
17.	ALA4417930	B	Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 5 uM by Western blotting based pulldown assay (Rvb = 100%)	Homo sapiens	1	ALA4417630	cell-based format	Patent Bioactivity Data
18.	ALA4417931	B	Activation of RhoA in human MDA-MB-435 cells assessed as remaining enzyme activity at 10 uM by Western blotting based pulldown assay (Rvb = 100%)	Homo sapiens	1	ALA4417630	cell-based format	Patent Bioactivity Data
19.	ALA4419720	B	Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced phospho-MLC formation at 50 uM incubated for 24 hrs by densitometry	Homo sapiens	1	ALA4419173	cell-based format	Patent Bioactivity Data
20.	ALA4419724	B	Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced focal adhesion complex formation at 50 uM by anti-vinculin antibody staining based assay	Homo sapiens	1	ALA4419173	cell-based format	Patent Bioactivity Data