Enhancement of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as accumulation of [3H]taurocholate at 10 uM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by liquid scintillation counting
Enhancement of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as accumulation of [3H]taurocholate at 1 uM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by liquid scintillation counting
TP_TRANSPORTER: inhibition of Taurocholate uptake(Rifampicin SV 30uM, 50 % of control) in membrane vesicles prepared from High Five cells infected with the ABCB11 baculovirus
TP_TRANSPORTER: inhibition of Taurocholate uptake(Glibenclamide 30uM, 50 % of control) in membrane vesicles prepared from High Five cells infected with the ABCB11 baculovirus
TP_TRANSPORTER: inhibition of Taurochenodeoxycholate uptake(CsA 30uM, 30 % of control) in membrane vesicles prepared from High Five cells infected with the ABCB11 baculovirus
Restoration of wild type human BSEP trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as decrease in [3H]taurocholate accumulation at 10 to 100 uM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by liquid scintillation counting
Restoration of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as accumulation of [3H]taurocholate at 100 uM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by liquid scintillation counting
Restoration of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as accumulation of [3H]taurocholate at 10 uM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by liquid scintillation counting
Restoration of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as decrease in [3H]taurocholate accumulation preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by liquid scintillation counting
Restoration of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as increase in [3H]taurocholate transport measuring apparent efflux clearance across the apical membrane at 1 mM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by transcellular transport assay relative to control
Restoration of human BSEP E297G mutant trafficking to cell membrane in MDCK2 cells co-expressing NTCP assessed as increase in [3H]taurocholate transport measuring apparent efflux clearance across the apical membrane at 10 uM preincubated for 24 hrs prior incubation with [3H]taurocholate measured after 2 hrs by transcellular transport assay relative to control