| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1023508 | B | Activation of PhoQ in Salmonella enterica serovar Typhimurium CS102 expressing PhoN fusion protein assessed as initiation of acid phosphatase reporter enzyme expression at 1 ug/ml in presence of 1 mM magnesium | Salmonella enterica subsp. enterica serovar Typhimurium | 6 | ALA1151446 | assay format | Scientific Literature | |
| 2. | ALA2184192 | B | Inhibition of Salmonella typhimurium sensor kinase PhoQ | Salmonella enterica subsp. enterica serovar Typhimurium | 1 | ALA2176884 | assay format | Scientific Literature | |
| 3. | ALA4186205 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged DHp-catalytic domain PhoQ (257 to 487 residues) autophosphorylation expressed in Escherichia coli BL21 (DE3) preincubated for 10 mins followed by [gamma-32P]-ATP addition after 30 mins by phosphotransferase dot blot assay | Salmonella enterica subsp. enterica serovar Typhimurium | 13 | ALA4184156 | assay format | Scientific Literature | |
| 4. | ALA4186206 | B | Binding affinity to recombinant Salmonella typhimurium 15N-labeled PhoQ catalytic domain (332 to 487 residues) expressed in Escherichia coli XA90 assessed as chemical shift perturbation by HSQC NMR analysis | Salmonella enterica subsp. enterica serovar Typhimurium | 1 | ALA4184156 | assay format | Scientific Literature | |
| 5. | ALA4186207 | B | Displacement of TNP-ATP from recombinant Salmonella typhimurium GST-tagged DHp-catalytic domain PhoQ (332 to 487 residues) expressed in Escherichia coli XA90 aafter 10 mins by fluorescence assay | Salmonella enterica subsp. enterica serovar Typhimurium | 1 | ALA4184156 | assay format | Scientific Literature | |
| 6. | ALA4186208 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged DHp-catalytic domain PhoQ (257 to 487 residues) autophosphorylation expressed in Escherichia coli BL21(DE3) at 500 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 30 mins by phosphotransferase dot blot assay relative to control | Salmonella enterica subsp. enterica serovar Typhimurium | 15 | ALA4184156 | assay format | Scientific Literature | |
| 7. | ALA4186210 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged DHp-catalytic domain PhoQ (257 to 487 residues) autophosphorylation expressed in Escherichia coli BL21 (DE3) at 800 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 30 mins by phosphotransferase dot blot assay relative to control | Salmonella enterica subsp. enterica serovar Typhimurium | 2 | ALA4184156 | assay format | Scientific Literature | |
| 8. | ALA4186212 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged PhoQ (257 to 487 residues) expressed in Escherichia coli BL21 (DE3) assessed as enzyme aggregation at 400 uM after 30 mins by native-PAGE analysis | Salmonella enterica subsp. enterica serovar Typhimurium | 5 | ALA4184156 | assay format | Scientific Literature | |
| 9. | ALA4186215 | B | Inhibition of recombinant Salmonella typhimurium N-terminal His6-SUMO-tagged PhoQ (257 to 487 residues) expressed in Escherichia coli BL21 (DE3) assessed as enzyme aggregation at 800 uM after 30 mins by native-PAGE analysis | Salmonella enterica subsp. enterica serovar Typhimurium | 1 | ALA4184156 | assay format | Scientific Literature |
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