#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1920921	B	Binding affinity for GST-tagged human BCL2A1 expressed in Escherichia coli BL21 cells	Homo sapiens	2		cell-based format	Scientific Literature
2.	ALA1794435	F	PUBCHEM_BIOASSAY: Counterscreen of A1 inhibitors in wild-type Mouse Embryonic Fibroblasts Measured in Cell-Based System Using Plate Reader - 2045-04_Inhibitor_Dose_DryPowder_Activity_Set2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2526]		⋯		cell-based format	PubChem BioAssays
3.	ALA936499	B	Inhibition of BH3 peptide binding to human recombinant A1 by fluorescence polarization assay	Homo sapiens	1		single protein format	Scientific Literature
4.	ALA1031063	B	Displacement of F-BakBH3 from Bfl-1 by fluorescence polarization assay		10		single protein format	Scientific Literature
5.	ALA1106538	B	Displacement of FITC-labeled Bak BH3 peptide from Bfl-1 after 30 mins by fluorescence polarization assay		3		single protein format	Scientific Literature
6.	ALA1273076	B	Inhibition of N-terminal FITC BH3 peptide binding to GST-tagged Bfl1 by fluorescence polarization assay		34		single protein format	Scientific Literature
7.	ALA1273077	B	Inhibition of N-terminal FITC BH3 peptide binding to GST-tagged Bfl1 by time resolved-FRET assay		34		single protein format	Scientific Literature
8.	ALA1273518	B	Displacement of F-BakBH3 from Bfl-1 after 30 mins by fluorescence polarization assay		12		single protein format	Scientific Literature
9.	ALA2173037	B	Inhibition of GST-tagged recombinant Bfl1 interaction with biotinylated Bim peptide by ELISA based competitive binding assay		8		single protein format	Scientific Literature
10.	ALA3266883	B	Displacement of Flu-BID/FAM-BID from His-tagged A1-BFL-1 (1 to 151) (unknown origin) expressed in Escherichia coli BL21(DE3) after 3 hrs by fluorescence polarization assay	Homo sapiens	7		assay format	Scientific Literature
11.	ALA3417965	B	Inhibition of BCL2-A1 (unknown origin) after 1 hr by TR-FRET assay	Homo sapiens	1		single protein format	Scientific Literature
12.	ALA3707566	B	Fluorescence Polarization Assay: A Bak BH3 peptide (F-BakBH3) (GQVGRQLAIIGDDINR (SEQ ID NO:1)) was labeled at the N-terminus with fluorescein isothiocyanate (FITC) (Molecular Probes) and purified by HPLC. For competitive binding assays, 100 nM GST-BCL-XL DTM protein was preincubated with the tested compound at varying concentrations in 47.5 uL PBS (pH=7.4) in 96-well black plates at room temperature for 10 min, then 2.5 uL of 100 nM FITC-labeled Bak BH3 peptide was added to produce a final volume of 50 uL. The wild-type and mutant Bak BH3 peptides were included in each assay plate as positive and negative controls, respectively. After 30 min incubation at room temperature, the polarization values in millipolarization units were measured at excitation/emission wavelengths of 480/535 nm with a multilabel plate reader (PerkinElmer). IC50 was determined by fitting the experimental data to a sigmoidal dose-response nonlinear regression model.	Homo sapiens	10		single protein format	BindingDB Database
13.	ALA3877061	B	Binding affinity to recombinant human C-terminal His6-tagged Bcl2A1 (1 to 152 residues) expressed in Escherichia coli assessed as adduct formation at 0.5 to 10 uM measured after 2 hrs by denaturing SDS-PAGE analysis	Homo sapiens	1		assay format	Scientific Literature
14.	ALA3877062	B	Binding affinity to recombinant human C-terminal His6-tagged Bcl2A1 (1 to 152 residues) expressed in Escherichia coli assessed as adduct formation at 2 uM measured after 2 hrs by denaturing SDS-PAGE analysis	Homo sapiens	1		assay format	Scientific Literature
15.	ALA3877063	B	Binding affinity to recombinant human C-terminal His6-tagged Bcl2A1 (1 to 152 residues) at Cys55 residue expressed in Escherichia coli assessed as adduct formation at 8 uM incubated 2 hrs prior to SDS-PAGE in-gel trypsin digestion by LC-MS/MS analysis	Homo sapiens	1		assay format	Scientific Literature
16.	ALA3877069	B	Inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli assessed as half life of Bcl2A1 to FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding at 500 nM by fluorescence polarization assay	Homo sapiens	1		assay format	Scientific Literature
17.	ALA3877070	B	Inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli at 500 nM measured after 90 mins by fluorescence polarization assay	Homo sapiens	1		assay format	Scientific Literature
18.	ALA3877071	B	Inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli measured after 2 hrs by fluorescence polarization assay	Homo sapiens	2		assay format	Scientific Literature
19.	ALA3877072	B	Reversible inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli preincubated for 1 hr with 2 fold diluted Bcl2A1 prior to FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 addition measured every minute over 3.5 hrs by fluorescence polarization assay	Homo sapiens	1		assay format	Scientific Literature
20.	ALA3877073	B	Irreversible inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli preincubated for 1 hr with 2 fold diluted Bcl2A1 prior to FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 addition measured every minute over 3.5 hrs by fluorescence polarization assay	Homo sapiens	1		assay format	Scientific Literature