#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3427982	Binding	Inhibition of calf thymus topoisomerase-1 assessed as conversion of negatively supercoiled plasmid DNA pBR322 to relaxed DNA at 100 uM after 30 mins by agarose gel electrophoresis	Bos taurus	1	ALA3425454	single protein format	Scientific Literature
2.	ALA1167314	Binding	Inhibition of calf thymus DNA topoisomerase 1-mediated pBR322 DNA cleavage	Bos taurus	4	ALA1165875	single protein format	Scientific Literature
3.	ALA1167315	Binding	Inhibition of calf thymus DNA topoisomerase 1-mediated pBR322 DNA cleavage at 1 uM	Bos taurus	1	ALA1165875	single protein format	Scientific Literature
4.	ALA1174240	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 by agarose gel electrophoresis	Bos taurus	2	ALA1177840	single protein format	Scientific Literature
5.	ALA1174244	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 at 1 uM by agarose gel electrophoresis	Bos taurus	1	ALA1177840	single protein format	Scientific Literature
6.	ALA1174245	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 at 50 uM by agarose gel electrophoresis	Bos taurus	1	ALA1177840	single protein format	Scientific Literature
7.	ALA1787592	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 at 10 uM after 15 mins using ethidium bromide staining by UV transilluminator analysis	Bos taurus	7	ALA1781993	single protein format	Scientific Literature
8.	ALA1787593	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 assessed as formation of nicked DNA at 1 to 50 uM after 15 mins using ethidium bromide staining by UV transilluminator	Bos taurus	2	ALA1781993	single protein format	Scientific Literature
9.	ALA1787594	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 assessed as increase in cleavable complex level after 15 mins using ethidium bromide staining by UV transilluminator analysis	Bos taurus	3	ALA1781993	single protein format	Scientific Literature
10.	ALA1061529	Binding	Inhibition of calf thymus topoisomerase 1-mediated supercoiled pBR322DNA relaxation activity	Bos taurus	1	ALA1152447	single protein format	Scientific Literature
11.	ALA1061530	Binding	Inhibition of calf thymus topoisomerase 1-mediated supercoiled pBR322DNA relaxation activity at 10 uM	Bos taurus	1	ALA1152447	single protein format	Scientific Literature
12.	ALA2166599	Binding	Inhibition of calf thymus topoisomerase 1-mediated relaxation of supercoiled pBR322 DNA after 15 mins by agarose gel electrophoresis	Bos taurus	1	ALA2163298	single protein format	Scientific Literature
13.	ALA2343682	Binding	Inhibition of calf thymus topoisomerase-1-mediated supercoiled plasmid DNA relaxation after 15 mins by agarose gel electrophoresis	Bos taurus	1	ALA2331235	single protein format	Scientific Literature
14.	ALA2343683	Binding	Inhibition of calf thymus topoisomerase-1-mediated DNA intercalation using relaxed pBR322 after 15 mins	Bos taurus	1	ALA2331235	single protein format	Scientific Literature
15.	ALA4138596	Binding	Inhibition of calf thymus topoisomerase 1 assessed as suppression of relaxation of supercoiled pBR322 DNA at 100 uM incubated for 0.5 hrs by bromophenol blue and xylene cyanol FF staining based electrophoresis method	Bos taurus	14	ALA4138059	single protein format	Scientific Literature
16.	ALA4138597	Binding	Potency index, ratio of enzyme inhibition of securinine to enzyme inhibition of test compound for inhibition of calf thymus topoisomerase 1 assessed as suppression of relaxation of supercoiled pBR322 DNA at 100 uM incubated for 0.5 hrs by bromophenol blue and xylene cyanol FF staining based electrophoresis method	Bos taurus	1	ALA4138059	single protein format	Scientific Literature
17.	ALA4138598	Binding	Inhibition of calf thymus topoisomerase 1 assessed as reduction in topo1/DNA complex formation at 50 to 400 uM pre-incubated for 0.33 hrs before addition of supercoiled pBR322 DNA substrate by EMSA	Bos taurus	1	ALA4138059	single protein format	Scientific Literature
18.	ALA4138599	Binding	Inhibition of calf thymus topoisomerase 1 assessed as increase in nicked DNA at 50 uM using supercoiled pBR322 DNA incubated for 0.5 hrs by ethidium bromide staining based DNA-cleavage assay	Bos taurus	4	ALA4138059	single protein format	Scientific Literature
19.	ALA4138600	Binding	Inhibition of calf thymus topoisomerase 1 assessed as increase in nicked DNA at 10 uM using supercoiled pBR322 DNA incubated for 0.5 hrs by ethidium bromide staining based DNA-cleavage assay	Bos taurus	5	ALA4138059	single protein format	Scientific Literature
20.	ALA4141854	Binding	Inhibition of calf thymus DNA topoisomerase 1 at 20 uM using pBR322 DNA as substrate after 30 mins by agarose gel electrophoresis method	Bos taurus	4	ALA4138165	single protein format	Scientific Literature