| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1054735 | B | Inhibition of JMJD2D-mediated H3K9 demethylation activity at 3 mM | 4 | ALA1152903 | single protein format | Scientific Literature | ||
| 2. | ALA1054736 | B | Inhibition of JMJD2D-mediated H3K9 demethylation activity at 1 mM | 4 | ALA1152903 | single protein format | Scientific Literature | ||
| 3. | ALA1055465 | F | Inhibition of JMJD2-mediated H3K36 demethylation in human 293T cells at 2.5 mM | 2 | ALA1152903 | cell-based format | Scientific Literature | ||
| 4. | ALA1055466 | F | Inhibition of JMJD2-mediated H3K9 demethylation in human 293T cells at 2.5 mM | 2 | ALA1152903 | cell-based format | Scientific Literature | ||
| 5. | ALA3363703 | B | Inhibition of purified recombinant KDM4D (1 to 350 aa) (unknown origin) expressed in Escherichia coli using H3K9me3 peptide as substrate after 30 mins by FDH-coupled assay | Homo sapiens | 2 | ALA3352546 | assay format | Scientific Literature | |
| 6. | ALA3772966 | B | Inhibition of KDM4D (unknown origin) by RFMS assay | Homo sapiens | 7 | ALA3769320 | single protein format | Scientific Literature | |
| 7. | ALA3776338 | B | Inhibition of KDM4D (unknown origin) expressed in Escherichia coli using H3 (1 to 21 residues) K9Me3-GGK-Biotin KDM4 Cognate Ligand/10 uM alpha-ketoglutarate as substrate/cofactor preincubated for 5 mins followed by substrate addition measured after 20 mins by alphascreen assay | Homo sapiens | 6 | ALA3774347 | assay format | Scientific Literature | |
| 8. | ALA3778165 | B | Inhibition of human KDM4D (11 to 341 residues) using H3K9Me3 peptide as substrate assessed as demethylation of substrate by Rapidfire mass spectrometric analysis | Homo sapiens | 10 | ALA3774367 | single protein format | Scientific Literature | |
| 9. | ALA4022350 | B | Inhibition of KDM4D (unknown origin) at 10 uM using biotinylated H3 derived peptide as substrate preincubated for 15 mins followed by substrate addition measured after 1 hr by AlphaLISA method | Homo sapiens | 22 | ALA4020786 | single protein format | Scientific Literature | |
| 10. | ALA4022354 | B | Inhibition of KDM4D (unknown origin) using biotinylated H3 derived peptide as substrate preincubated for 15 mins followed by substrate addition measured after 1 hr by AlphaLISA method | Homo sapiens | 4 | ALA4020786 | single protein format | Scientific Literature | |
| 11. | ALA4193855 | B | Inhibition of KDM4D (unknown origin) using biotinylated H3K9me3 peptide and alpha-ketoglutarate as substrate pretreated for 1 hr followed by substrate addition measured after 20 mins by LANCE TR-FRET assay | Homo sapiens | 1 | ALA4190335 | single protein format | Scientific Literature | |
| 12. | ALA4223301 | B | Inhibition of KDM4D (unknown origin) | Homo sapiens | 1 | ALA4219204 | single protein format | Scientific Literature | |
| 13. | ALA4832319 | B | Inhibition of human KDM4D expressed in Escherichia coli BL21 (DE3) at 1 uM using biotinylated H3-derived peptide and 2-OG as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by AlphaLISA assay | Homo sapiens | 71 | ALA4831501 | assay format | Scientific Literature | |
| 14. | ALA4832320 | B | Inhibition of human KDM4D expressed in Escherichia coli BL21 (DE3) using biotinylated H3-derived peptide and 2-OG as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by AlphaLISA assay | Homo sapiens | 54 | ALA4831501 | assay format | Scientific Literature | |
| 15. | ALA4832321 | B | Inhibition of human KDM4D expressed in Escherichia coli BL21 (DE3) assessed as equilibrium dissociation constant measured by isothermal titration calorimetry assay | Homo sapiens | 1 | ALA4831501 | assay format | Scientific Literature | |
| 16. | ALA4832322 | B | Binding affinity to human KDM4D expressed in Escherichia coli BL21(DE3) assessed as negative thermal shift in melting temperature measured for 60 mins by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA4831501 | assay format | Scientific Literature | |
| 17. | ALA4832323 | B | Mixed inhibition of human KDM4D expressed in Escherichia coli BL21(DE3) using H3(1-21)K9me3 peptide and varying concentrations of 2-OG as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by Michaelis-Menten plot based analysis | Homo sapiens | 1 | ALA4831501 | assay format | Scientific Literature | |
| 18. | ALA4832349 | B | Inhibition of GST-tagged KDM4D (unknown origin) expressed in Escherichia coli DH5alpha assessed as histone demethylation activity using H3K9me3 as substrate incubated for 4 hrs in presence of FLAG-histone H3 measured by Western blot analysis | Homo sapiens | 1 | ALA4831501 | assay format | Scientific Literature | |
| 19. | ALA4832350 | B | Inhibition of KDM4D in human HCT-116 cells assessed as reduction in Gli1 protein expression incubated for 48 hrs measured by Western blot analysis | Homo sapiens | 1 | ALA4831501 | cell-based format | Scientific Literature | |
| 20. | ALA4832351 | B | Inhibition of KDM4D in human HCT-116 cells assessed as reduction in beta-catenin protein expression incubated for 48 hrs measured by Western blot analysis | Homo sapiens | 1 | ALA4831501 | cell-based format | Scientific Literature |
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