#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1051662	B	Inhibition of rat recombinant PDE10A transfected in Sf9 cells	Rattus norvegicus	18	ALA1152448	cell-based format	Scientific Literature
2.	ALA1820393	B	Inhibition of recombinant rat PDE10A expressed in Sf9 cells using [3H]cAMP after 30 mins by scintillation proximity assay	Rattus norvegicus	17	ALA1817636	cell-based format	Scientific Literature
3.	ALA1827314	B	Inhibition of rat PDE10A	Rattus norvegicus	4	ALA1821671	single protein format	Scientific Literature
4.	ALA1919970	B	Inhibition of rat recombinant PDE10A expressed in baculovirus infected insect Sf9 cells using [3H]cAMP as substrate after 60 mins by scintillation counting	Rattus norvegicus	24	ALA1914437	cell-based format	Scientific Literature
5.	ALA2072667	B	Binding affinity to PDE10A in Sprague-Dawley rat striatum by TopCount analysis	Rattus norvegicus	1	ALA2069179	single protein format	Scientific Literature
6.	ALA2072668	B	Binding affinity to PDE10A in Sprague-Dawley rat striatum assessed per mg of protein by TopCount analysis	Rattus norvegicus	1	ALA2069179	single protein format	Scientific Literature
7.	ALA2072669	B	Displacement of [3H]5-(6,7-dimethoxycinnolin-4-yl)-N-isopropyl-3-methylpyridin-2-amine from PDE10A in Sprague-Dawley rat striatum	Rattus norvegicus	2	ALA2069179	single protein format	Scientific Literature
8.	ALA2072677	B	Competitive inhibition of PDE10A in Sprague-Dawley rat striatum at 5 pM to 100 nM	Rattus norvegicus	1	ALA2069179	single protein format	Scientific Literature
9.	ALA2187449	B	Inhibition of rat recombinant PDE10A2 expressed in baculovirus infected SF21 cells using [3H]cAMP as substrate after 60 mins by scintillation proximity assay	Rattus norvegicus	3	ALA2177053	cell-based format	Scientific Literature
10.	ALA2320476	B	Inhibition of rat PDE10A expressed in Sf9 cells by scintillation proximity assay	Rattus norvegicus	34	ALA2311279	cell-based format	Scientific Literature
11.	ALA3096516	B	Inhibition of recombinant rat PDE10A expressed in baculovirus infected insect SF9 cells using [3H]-cAMP as substrate after 60 mins by scintillation proximity assay	Rattus norvegicus	1	ALA3091315	cell-based format	Scientific Literature
12.	ALA3266764	B	Inhibition of rat recombinant PDE10A expressed in baculovirus infected Sf9 cells using [3H]cAMP as substrate after 60 mins by TopCount scintillation counting analysis	Rattus norvegicus	23	ALA3259706	cell-based format	Scientific Literature
13.	ALA3390271	B	Inhibition of rat PDE10A expressed in Sf9 cells incubated for 60 mins using [3H]-cAMP substrate by scintillation counting method	Rattus norvegicus	11	ALA3351311	cell-based format	Scientific Literature
14.	ALA3368978	B	Inhibition of rat recombinant GST-tagged PDE10A using [3H]-cAMP as substrate by scintillation proximity assay	Rattus norvegicus	14	ALA3352660	single protein format	Scientific Literature
15.	ALA3396559	B	Inhibition of rat recombinant PDE10A expressed in Sf9 cells using [3H]cAMP substrate incubated for 60 mins by topcount scintillation counting method	Rattus norvegicus	34	ALA3393008	cell-based format	Scientific Literature
16.	ALA3430113	B	Inhibition of 6-His-tagged rat recombinant PDE10A expressed in Sf9 cells using [3H]cAMP incubated for 60 mins by scintillation proximity assay	Rattus norvegicus	11	ALA3425427	cell-based format	Scientific Literature
17.	ALA3635513	B	Inhibition of rat PDE10A using [3H]cAMP/cAMP as substrate assessed as hydrolysis of [3H]cAMP to [3H]AMP after 30 mins by scintillation proximity assay	Rattus norvegicus	1	ALA3632475	single protein format	Scientific Literature
18.	ALA3705282	B	Enzyme Inhibition Assay: Enzyme activity: An IMAP TR-FRET assay was used to analyze the enzyme activity (Molecular Devices Corp., Sunnyvale, Calif).	Rattus norvegicus	320	ALA3639166	single protein format	BindingDB Database
19.	ALA3706152	B	Enzyme Inhibition Assay: An IMAP TR-FRET assay was used to analyze the enzyme activity (Molecular Devices Corp., Sunnyvale Calif.). 5 μL of serial diluted PDE10A (BPS Bioscience, San Diego, Calif.) or tissue homogenate was incubated with equal volumes of diluted fluorescein labeled cAMP or cGMP for 60 min in 384-well polystyrene assay plates (Corning, Corning, N.Y.) at room temperature. After incubation, the reaction was stopped by adding 60 μL of diluted binding reagents and was incubated for 3 hours to overnight at room temperature. The plates were read on an Envision (Perkin Elmer, Waltham, Mass.) for time resolved fluorescence resonance energy transfer.	Rattus norvegicus	351	ALA3639057	assay format	BindingDB Database
20.	ALA3706138	B	Scintillation Proximity Assay: The ability of a compound to inhibit PDE10 enzymatic activity can be demonstrated by any number of assays that are known in the art. The products of Examples 1-175 were tested in the assay described below.PDE10 activity was measured using a scintillation assay (SPA-based method) similar to that previously described by Seeger, T. F. et al., Brain Research 985 (2003) 113-126. The compounds' relative activity as PDE10 inhibitors was investigated by assaying a fixed amount of enzyme in the presence of the test compound and low substrate concentration (cAMP) such that an IC50 could be determined. More specifically, this assay uses a Scintillation Proximity Assay (SPA) to measure the inhibition of rat1 and human recombinant2 PDE10 enzyme activity by compounds in vitro (preparation of enzymes described below). The assay is performed in a 384-well format with 50 uL assay buffer (50 mM TRIS pH 7.5; 1.3 mM MgCl2; 0.01% Brij) containing enough PDE10.	Rattus norvegicus	168	ALA3638917	single protein format	BindingDB Database