#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1053220	Binding	Activation of human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in presence of fumarate	Homo sapiens	1	ALA1153457	assay format	Scientific Literature
2.	ALA1053221	Binding	Activation of human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in absence of fumarate	Homo sapiens	1	ALA1153457	assay format	Scientific Literature
3.	ALA1053224	Binding	Inhibition of wild type human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in absence of fumarate	Homo sapiens	3	ALA1153457	assay format	Scientific Literature
4.	ALA1053225	Binding	Inhibition of wild type human recombinant mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as residual enzyme activity by spectrophotometry in presence of fumarate	Homo sapiens	3	ALA1153457	assay format	Scientific Literature
5.	ALA1053226	Binding	Activation of human recombinant wild type mitochondrial NADP-ME expressed in Escherichia coli BL21 assessed as one-half maximal activation by spectrophotometry	Homo sapiens	2	ALA1153457	assay format	Scientific Literature
6.	ALA5225647	Binding	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	34	ALA5223163	single protein format	Scientific Literature
7.	ALA5225652	Binding	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 1 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	16	ALA5223163	single protein format	Scientific Literature
8.	ALA5225653	Binding	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 10 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	11	ALA5223163	single protein format	Scientific Literature
9.	ALA5225656	Binding	Competitive inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using varying concentrations of L-malate as substrate in presence of fixed concentration of beta-NADP+ and measured for 10 mins by Lineweaver-Burk plot analysis	Homo sapiens	1	ALA5223163	single protein format	Scientific Literature
10.	ALA5225657	Binding	Binding affinity to ME3 in human BXPC-3 live cells assessed as change in melting temperature at 100 uM preincubated with live cells for 24 hrs followed by exposure to varying temperature by CETSA analysis	Homo sapiens	1	ALA5223163	cell-based format	Scientific Literature
11.	ALA5225658	Binding	Uncompetitive inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using varying concentrations of beta-NADP+ as substrate in presence of fixed concentration of L-malate and measured for 10 mins by Lineweaver-Burk plot analysis	Homo sapiens	1	ALA5223163	single protein format	Scientific Literature
12.	ALA5225659	Binding	Binding affinity to ME3 in human BXPC-3 cell lysates assessed as change in melting temperature at 100 uM preincubated with cell lysates for 2 hrs followed by exposure to varying temperature by CETSA analysis	Homo sapiens	1	ALA5223163	subcellular format	Scientific Literature
13.	ALA5225660	Binding	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 0.1 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	5	ALA5223163	single protein format	Scientific Literature