#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA654919	B	Inhibitory activity against Escherichia coli HA208 Class-III SHV-1 type beta-lactamase enzyme	Escherichia coli	16	ALA1151538	single protein format	Scientific Literature
2.	ALA653640	B	Rate of hydrolysis in 0.1 M sodium phosphate buffer at pH=7.0 by Beta lactamase (SHV-2)	Proteus vulgaris	1	ALA1129566	single protein format	Scientific Literature
3.	ALA876075	B	Rate of hydrolysis in 0.1 M sodium phosphate buffer at pH=7.0 by Beta lactamase (SHV-4)	Proteus vulgaris	1	ALA1129566	single protein format	Scientific Literature
4.	ALA653644	B	Rate of hydrolysis in 0.1 M sodium phosphate buffer at pH=7.0 by Beta lactamase (SHV-2)	Proteus vulgaris	1	ALA1129566	single protein format	Scientific Literature
5.	ALA653645	B	Rate of hydrolysis in 0.1 M sodium phosphate buffer at pH=7.0 by Beta lactamase (SHV-4)	Proteus vulgaris	1	ALA1129566	single protein format	Scientific Literature
6.	ALA653464	B	Compound was tested for inhibition of Beta-lactamase from SHV-2		1	ALA1129642	single protein format	Scientific Literature
7.	ALA919581	B	Inhibition of Escherichia coli SHV1	Escherichia coli	1	ALA1149281	single protein format	Scientific Literature
8.	ALA913064	B	Binding affinity at Beta-lactamase SHV1 expressed in Escherichia coli DH10B cells	Escherichia coli	3	ALA1146195	cell-based format	Scientific Literature
9.	ALA894369	B	Inhibition of Escherichia coli SHV1	Escherichia coli	10	ALA1148735	single protein format	Scientific Literature
10.	ALA1267571	B	Activity at Escherichia coli beta-lactamase SHV-1	Escherichia coli	8	ALA1255455	single protein format	Scientific Literature
11.	ALA1267572	B	Ratio of Kcat to Km in Escherichia coli beta-lactamase SHV-1	Escherichia coli	8	ALA1255455	single protein format	Scientific Literature
12.	ALA3887058	B	Cell Free Inhibition Assay: The inhibitory concentrations (IC50, [uM]) of the beta -lactamase inhibitors against purified TEM-1, SHV-1 and AmpC beta -lactamases are assessed by determining the concentration of inhibitor at which 50% of the nitrocefin hydrolysis is inhibited by the particular enzyme. Assays are performed with beta -lactamases expressed in the pET system (Novagen, San Diego, Calif.) without signal peptides. They contain an N-terminal hexa-Histidine tag that is used for purification on Ni-NTA (Qiagen, Hilden, Germany). The compounds are prepared as 50 mM stocks in DMSO and diluted into buffer P1 (50 mM phosphate, pH 7) to a final concentration of 10% DMSO. All further dilutions are done in P2 (P1 with 10% DMSO). Enzyme and compound dilutions are pre-incubated for 10 min at 37 C. and the reaction is started with the addition of pre-warmed (37 C.) nitrocefin at a final concentration of 50 mM. The change in absorption at 490 nm is followed at 37 C. for 10 min with 30 s intervals.		33	ALA3886147	assay format	BindingDB Database