#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA862863	B	Activity against Escherichia coli nitroreductase	Escherichia coli	2	ALA1138079	single protein format	Scientific Literature
2.	ALA4316689	A	Susbtrate activity at Escherichia coli nitroreductase assessed as half life	Escherichia coli	2	ALA4316582	single protein format	Scientific Literature
3.	ALA4316691	B	Inhibition of Escherichia coli nitroreductase expressed in human HCT116 cells	Escherichia coli	1	ALA4316582	cell-based format	Scientific Literature
4.	ALA4376796	A	Prodrug conversion in PBS containing 5% DMSO assessed as Escherichia coli nitroreductase-mediated activation by measuring increased consumption of prodrug at 0.01 mg/ml at pH 7.4 in presence of NADH by HPLC analysis	Escherichia coli	2	ALA4373749	single protein format	Scientific Literature
5.	ALA4376799	A	Prodrug conversion in PBS containing 5% DMSO assessed as Escherichia coli nitroreductase-mediated activation by measuring release of ethyl (6R)-6-[(2-chloro-4-fluorophenyl)sulfamoyl]cyclohexene-1-carboxylate parent drug at 0.01 mg/ml at pH 7.4 in presence of NADH by HPLC analysis	Escherichia coli	2	ALA4373749	single protein format	Scientific Literature
6.	ALA4376800	A	Prodrug conversion in PBS containing 5% DMSO assessed as Escherichia coli nitroreductase-mediated activation by measuring reduced amine intermediate metabolite formation at 0.01 mg/ml at pH 7.4 in presence of NADH by HPLC analysis	Escherichia coli	2	ALA4373749	single protein format	Scientific Literature
7.	ALA4380227	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as Kcat using NADH as cofactor in Tris-Cl buffer at pH 7.5 by spectrophotometric method	Escherichia coli	2	ALA4376859	single protein format	Scientific Literature
8.	ALA4380228	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as Kcat upto 1 mM using NADH as cofactor in sodium phosphate buffer at pH 7.5 incubated for 5 mins by RP-HPLC	Escherichia coli	1	ALA4376859	single protein format	Scientific Literature
9.	ALA4380234	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by spectrophotometric method based Michaelis-Menten plot analysis	Escherichia coli	2	ALA4376859	single protein format	Scientific Literature
10.	ALA4380236	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as Km upto 1 mM using NADH as cofactor in sodium phosphate buffer at pH 7.5 incubated for 5 mins by RP-HPLC	Escherichia coli	1	ALA4376859	single protein format	Scientific Literature
11.	ALA4380241	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as ratio of Kcat/Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by spectrophotometric method based Michaelis-Menten plot analysis	Escherichia coli	2	ALA4376859	single protein format	Scientific Literature
12.	ALA4380242	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as ratio of Kcat/Km upto 1 mM using NADH as cofactor in sodium phosphate buffer at pH 7.5 incubated for 5 mins by RP-HPLC	Escherichia coli	1	ALA4376859	single protein format	Scientific Literature
13.	ALA4380248	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as ratio of Kcat/Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by spectrophotometric method based Michaelis-Menten plot analysis relative to CB1954/NfsB	Escherichia coli	2	ALA4376859	single protein format	Scientific Literature
14.	ALA4380249	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as ratio of Kcat/Km upto 1 mM using NADH as cofactor in sodium phosphate buffer at pH 7.5 incubated for 5 mins by RP-HPLC relative to CB1954/NfsB	Escherichia coli	1	ALA4376859	single protein format	Scientific Literature
15.	ALA4394202	B	Substrate activity at Escherichia coli nitroreductase nfsB assessed as Kcat using NADH as cofactor at 0 to 800 mM in Tris-Cl buffer at pH 7.5 by spectrophotometric method	Escherichia coli	3	ALA4393661	single protein format	Scientific Literature
16.	ALA4394203	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by spectrophotometric method	Escherichia coli	3	ALA4393661	single protein format	Scientific Literature
17.	ALA4394204	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as ratio of Kcat/Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by spectrophotometric method	Escherichia coli	3	ALA4393661	single protein format	Scientific Literature
18.	ALA4394208	A	Substrate activity at Escherichia coli nitroreductase nfsB assessed as ratio of Kcat/Km up to 80 mM using NADH as cofactor relative to CB1954/NfsB	Escherichia coli	2	ALA4393661	single protein format	Scientific Literature