| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1042900 | B | Activity at ecto-5'-nucleotidase | 1 | ALA1155272 | single protein format | Scientific Literature | ||
| 2. | ALA1805826 | B | Activity at human Ecto-5'-nucleotidase expressed in COS7 cells assessed as amount of inorganic phosphate released using ATP as the substrate at 100 uM after 15 mins by malachite green reagent method | Homo sapiens | 4 | ALA1799960 | cell-based format | Scientific Literature | |
| 3. | ALA1805833 | B | Inhibition of human Ecto-5'-nucleotidase expressed in COS7 cells assessed as amount of inorganic phosphate released using ATP as the substrate at 100 uM after 15 mins by malachite green reagent method | Homo sapiens | 4 | ALA1799960 | cell-based format | Scientific Literature | |
| 4. | ALA2411272 | B | Inhibition of human Ecto-5'-NT expressed in african green monkey COS7 cells using AMP as substrate at 0.1 mM preincubated for 10 mins measured after 10 mins | Homo sapiens | 21 | ALA2407035 | cell-based format | Scientific Literature | |
| 5. | ALA3090247 | B | Inhibition of human recombinant ecto-5'-nucleotidase expressed in african green monkey COS7 cells using adenosine monophosphate as substrate at 1 mM preincubated for 10 mins before substrate addition measured after 10 mins by capillary electrophoresis | Homo sapiens | 4 | ALA3085736 | cell-based format | Scientific Literature | |
| 6. | ALA3090249 | B | Inhibition of human recombinant ecto-5'-nucleotidase expressed in african green monkey COS7 cells using adenosine monophosphate as substrate preincubated for 10 mins before substrate addition measured after 10 mins by capillary electrophoresis | Homo sapiens | 9 | ALA3085736 | cell-based format | Scientific Literature | |
| 7. | ALA3256247 | B | Inhibition of 5'-nucleotidase (unknown origin) | Homo sapiens | 1 | ALA3244410 | single protein format | Scientific Literature | |
| 8. | ALA3364115 | B | Inhibition of AMPase activity of human ecto-5'-nucleotidase using AMP substrate relative to control | Homo sapiens | 1 | ALA3352222 | single protein format | Scientific Literature | |
| 9. | ALA3804264 | B | Inhibition of human Ecto-5'-nucleotidase transfected in COS7 cells preincubated for 10 mins followed by AMP addition measured after 10 mins | Homo sapiens | 13 | ALA3797053 | cell-based format | Scientific Literature | |
| 10. | ALA3804266 | B | Inhibition of human Ecto-5'-nucleotidase transfected in COS7 cells at 1 mM preincubated for 10 mins followed by AMP addition measured after 10 mins | Homo sapiens | 6 | ALA3797053 | cell-based format | Scientific Literature | |
| 11. | ALA3804268 | B | Uncompetitive inhibition of human Ecto-5'-nucleotidase transfected in COS7 cells at 0.5 to 2 uM preincubated for 10 mins followed by AMP addition measured after 10 mins by double-reciprocal plot analysis | Homo sapiens | 1 | ALA3797053 | cell-based format | Scientific Literature | |
| 12. | ALA3815631 | B | Inhibition of human recombinant Ecto-5'-nucleotidase expressed in CHO cells using AMP as substrate at >100 uM preincubated with substrate followed by protein addition and subsequent incubation for 30 mins by capillary electrophoresis | Homo sapiens | 1 | ALA3813632 | cell-based format | Scientific Literature | |
| 13. | ALA4119787 | B | Inhibition of human flag-tagged CD73 Thr376Ala mutant (1 to 552 residues) assessed as reduction in conversion of AMP to adenosine incubated for 10 mins followed by AMP addition measured after 30 mins by RapidFire mass spectrometric analysis | Homo sapiens | 6 | ALA4118087 | single protein format | Scientific Literature | |
| 14. | ALA4275460 | B | Inhibition of recombinant CD73 (27 to 549 residues) (unknown origin) expressed in baculovirus infected Sf9 insect cells assessed as reduction in conversion of AMP to adenosine incubated for 1 min followed by AMP addition measured after 3.5 mins by malachite green reagent based assay | Homo sapiens | 4 | ALA4270632 | cell-based format | Scientific Literature | |
| 15. | ALA4275461 | B | Inhibition of CD73 in human MDA-MB-231 cells assessed as reduction in conversion of AMP to adenosine incubated for 30 mins by malachite green reagent based assay | Homo sapiens | 4 | ALA4270632 | cell-based format | Scientific Literature | |
| 16. | ALA4275462 | B | Inhibition of CD73 in human NCI-H292 cells assessed as reduction in conversion of AMP to adenosine incubated for 30 mins by malachite green reagent based assay | Homo sapiens | 4 | ALA4270632 | cell-based format | Scientific Literature | |
| 17. | ALA4387945 | B | Competitive type of inhibition of human 5'-nucleotidase using IMP as substrate assessed as inhibitor constant for free enzyme by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4387703 | single protein format | Scientific Literature | |
| 18. | ALA4387946 | B | Non-competitive type of inhibition of human 5'-nucleotidase using IMP as substrate assessed as inhibitor constant for enzyme substrate complex by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4387703 | single protein format | Scientific Literature | |
| 19. | ALA4387947 | B | Competitive inhibition of 5'-nucleotidase (unknown origin) using varying level of IMP as substrate by Lineweaver-Burk plot analysis | Homo sapiens | 3 | ALA4387703 | single protein format | Scientific Literature | |
| 20. | ALA4387948 | B | Inhibition of recombinant cN-II (unknown origin) assessed as reduction in inorganic phosphate production at 200 uM pre-incubated for 10 mins followed by addition of IMP as substrate and measured after 5 mins by malachite green based assay relative to control | Homo sapiens | 20 | ALA4387703 | single protein format | Scientific Literature |
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