| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3424964 | Binding | Inhibition of human AKR1B10 using D-glucuronate as substrate by spectrophotometry | Homo sapiens | 1 | ALA3421614 | single protein format | Scientific Literature | |
| 2. | ALA3745355 | Binding | Inhibition of recombinant human AKR1B10 expressed in human HCT116 cells assessed as reduction in daunorubicinol production using daunorubicin as substrate at 20 uM incubated for 4 to 8 hrs by UHPLC based transient transfection assay | Homo sapiens | 4 | ALA3739262 | cell-based format | Scientific Literature | |
| 3. | ALA3745356 | Binding | Inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 cells using all-trans-retinal as substrate incubated for 15 mins by HPLC method | Homo sapiens | 6 | ALA3739262 | cell-based format | Scientific Literature | |
| 4. | ALA3745357 | Binding | Inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 (DE3) pLysS cells by pyridine-3-aldehyde reductase activity assay | Homo sapiens | 1 | ALA3739262 | cell-based format | Scientific Literature | |
| 5. | ALA3745358 | Binding | Inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 cells using all-trans-retinal as substrate at 20 uM incubated for 15 mins by HPLC method | Homo sapiens | 25 | ALA3739262 | cell-based format | Scientific Literature | |
| 6. | ALA3745360 | Binding | Non-competitive inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 cells using all-trans-retinal as substrate incubated for 15 mins by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA3739262 | cell-based format | Scientific Literature | |
| 7. | ALA3745362 | Binding | Inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 cells using daunorubicin as substrate incubated for 20 mins by UHPLC method | Homo sapiens | 3 | ALA3739262 | cell-based format | Scientific Literature | |
| 8. | ALA3745363 | Binding | Inhibition of recombinant human AKR1B10 expressed in human HCT116 cells assessed as reduction in daunorubicinol production using daunorubicin as substrate at 10 uM incubated for 4 to 8 hrs by UHPLC based transient transfection assay | Homo sapiens | 2 | ALA3739262 | cell-based format | Scientific Literature | |
| 9. | ALA3745364 | Binding | Inhibition of recombinant human AKR1B10 expressed in human HCT116 cells assessed as reduction in daunorubicinol production using daunorubicin as substrate incubated for 4 to 8 hrs by UHPLC based transient transfection assay | Homo sapiens | 1 | ALA3739262 | cell-based format | Scientific Literature | |
| 10. | ALA3745365 | Binding | Potency index, ratio of IC50 for flufenamic acid to IC50 for text compound for inhibition of recombinant human N-terminal His6-tagged AKR1B10 expressed in Escherichia coli BL21 cells using all-trans-retinal as substrate incubated for 15 mins by HPLC method | Homo sapiens | 3 | ALA3739262 | cell-based format | Scientific Literature | |
| 11. | ALA3821395 | ADME | Binding affinity to AKR1B10 in human HepG2 assessed as intensity fold change of cumulated normalized intensity of protein between capture and competition assay at 100 uM after 1 hr in presence of inactive Tcp-CC 14 by differential competition capture compound mass spectrometry | Homo sapiens | 4 | ALA3817783 | single protein format | Scientific Literature | |
| 12. | ALA1174247 | Binding | Inhibition of reductase activity of N-terminal 6His-tagged AKR1B10 expressed in Escherichia coli BL21(DE3) assessed as inhibition of NADPH linked pyridine-3-aldehyde reduction | 9 | ALA1177841 | assay format | Scientific Literature | ||
| 13. | ALA1174248 | Binding | Inhibition of dehydrogenase activity of N-terminal 6His-tagged AKR1B10 expressed in Escherichia coli BL21(DE3) assessed as inhibition of geraniol dehydrogenation in presence of saturating concentration of NADP+ | 4 | ALA1177841 | assay format | Scientific Literature | ||
| 14. | ALA1174249 | Binding | Selectivity ratio of compound to tolerstat for binding affinity to N-terminal 6His-tagged AKR1B10 | 1 | ALA1177841 | single protein format | Scientific Literature | ||
| 15. | ALA1174250 | Binding | Selectivity ratio of compound to isolithochloic acid for binding affinity to N-terminal 6His-tagged AKR1B10 | 1 | ALA1177841 | single protein format | Scientific Literature | ||
| 16. | ALA1174251 | Binding | Selectivity ratio of compound to bisdemethoxycurcumin for binding affinity to N-terminal 6His-tagged AKR1B10 | 1 | ALA1177841 | single protein format | Scientific Literature | ||
| 17. | ALA1174252 | Binding | Inhibition of AKR1B10 overexpressed in human HeLa cells assessed as inhibition of [1-14C]farnesol reduction at 1 uM pretreated for 2 hrs before [1-14C]farnesol challenge measured after 6 hrs | 1 | ALA1177841 | cell-based format | Scientific Literature | ||
| 18. | ALA1174253 | Binding | Inhibition of AKR1B10 overexpressed in BAEC assessed as enhancement of HNE-mediated cytotoxicity after 2 hrs treated 24 hrs before HNE challenge by MTT assay | 1 | ALA1177841 | assay format | Scientific Literature | ||
| 19. | ALA1174259 | Binding | Inhibition of AKR1B10 overexpressed in human HeLa cells assessed as inhibition of [1-14C]farnesol reduction at 0.1 uM pretreated for 2 hrs before [1-14C]farnesol challenge measured after 6 hrs | 1 | ALA1177841 | cell-based format | Scientific Literature | ||
| 20. | ALA3421434 | Binding | Inhibition of wild-type N-terminal 6-His tagged AKR1B10 (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as pyridine-3-aldehyde reduction by spectrophotometry | Homo sapiens | 3 | ALA3414556 | assay format | Scientific Literature |
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