# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA905355 | B | Inhibition of human DNA polymerase eta at 200 uM | Homo sapiens | 2 | ALA1143033 | single protein format | Scientific Literature | |
2. | ALA5111529 | B | Inhibition of human DNA polymerase theta pol domain (1819 to 2590 residues) expressed in Escherichia coli BL21 (DE3) cells preincubated for 15 mins followed by DNA and dNTP addition and measured after 120 mins by Picogreen dye fluorescence intensity analysis | Homo sapiens | 48 | ALA5108047 | cell-based format | Scientific Literature | |
3. | ALA5111533 | B | Inhibition of N-terminal His8-tagged/C-terminal FLAG-tagged full length human DNA polymerase theta ( 1 to 2590 residues) polymerase activity expressed in HEK2936E cells | Homo sapiens | 1 | ALA5108047 | cell-based format | Scientific Literature | |
4. | ALA5111534 | B | Inhibition of N-terminal His8-tagged/C-terminal FLAG-tagged full length human DNA polymerase theta ( 1 to 2590 residues) ATPase activity expressed in HEK2936E cells | Homo sapiens | 1 | ALA5108047 | cell-based format | Scientific Literature | |
5. | ALA5111542 | B | Inhibition of DNA polymerase theta in LIG4 knock out HEK293T cells assessed as decrease in MMEJ-mediated DNA repair preincubated for 1 day followed by lentivirus solution addition for 24 hrs prior to media replacement with compound and measured after 96 hrs by flow cytometry based traffic-light reporter assay | Homo sapiens | 1 | ALA5108047 | cell-based format | Scientific Literature | |
6. | ALA5111553 | B | Binding affinity to wild type human DNA polymerase theta 5LD domain assessed as thermal stabilisation by DSF assay | Homo sapiens | 2 | ALA5108047 | single protein format | Scientific Literature | |
7. | ALA5111554 | B | Binding affinity to wild type human DNA polymerase theta 5LD domain assessed as thermal stabilisation using dsDNA as substrate by DSF assay | Homo sapiens | 2 | ALA5108047 | single protein format | Scientific Literature | |
8. | ALA5111562 | B | Inhibition of human DNA polymerase theta using 5'-CACTGACTGTATGATG as primer and DNA 16/19 as substrate by FRET assay | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
9. | ALA5111567 | B | Inhibition of human DNA polymerase theta using DNA 16/19 as substrate at 5 nM of enzyme concentration | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
10. | ALA5111568 | B | Inhibition of human DNA polymerase theta using DNA 16/19 as substrate at 2 nM of enzyme concentration | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
11. | ALA5111569 | B | Inhibition of human DNA polymerase theta using DNA 16/19 as substrate at 1 nM of enzyme concentration | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
12. | ALA5111570 | B | Inhibition of human DNA polymerase theta using DNA 16/19 as substrate at 0.5 nM of enzyme concentration | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
13. | ALA5111571 | B | Inhibition of human DNA polymerase theta assessed as substrate Vmax using dNTP as substrate at 2.5 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
14. | ALA5111572 | B | Inhibition of human DNA polymerase theta assessed as substrate Vmax using dNTP as substrate at 5 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
15. | ALA5111573 | B | Inhibition of human DNA polymerase theta assessed as substrate Vmax using dNTP as substrate at 10 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
16. | ALA5111574 | B | Inhibition of human DNA polymerase theta assessed as substrate Vmax using dNTP as substrate at 20 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
17. | ALA5111575 | B | Inhibition of human DNA polymerase theta assessed as substrate Vmax using dNTP as substrate at 30 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
18. | ALA5111576 | B | Inhibition of human DNA polymerase theta assessed as substrate Vmax using dNTP as substrate at 40 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
19. | ALA5111577 | B | Inhibition of human DNA polymerase theta assessed as substrate Km using dNTP as substrate at 2.5 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature | |
20. | ALA5111578 | B | Inhibition of human DNA polymerase theta assessed as substrate Km using dNTP as substrate at 5 nM | Homo sapiens | 1 | ALA5108047 | single protein format | Scientific Literature |