#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1174394	Binding	Inhibition of EIF2AK1 at 10 uM		2	ALA1177794	single protein format	Scientific Literature
2.	ALA4351892	Binding	Inhibition of recombinant human HRI (140 to end residues) at 10 uM using RSRSRSRSRSRSRSR as substrate measured after 120 mins in presence of [gamma33P]ATP by scintillation counting method	Homo sapiens	1	ALA4350961	single protein format	Scientific Literature
3.	ALA4357859	Binding	Binding affinity to wild-type human partial length EIF2AK1 (Q147 to E603 residues) expressed in mammalian expression system assessed as residual binding level at 1 uM by Kinomescan method relative to control	Homo sapiens	1	ALA4354843	assay format	Scientific Literature
4.	ALA1908777	Binding	Binding constant for EIF2AK1 kinase domain		72	ALA1908390	single protein format	Scientific Literature
5.	ALA3412170	Binding	Inhibition of HRI (unknown origin)	Homo sapiens	2	ALA3407336	single protein format	Scientific Literature
6.	ALA1942915	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 40 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	1	ALA1938338	cell-based format	Scientific Literature
7.	ALA1942916	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 20 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	1	ALA1938338	cell-based format	Scientific Literature
8.	ALA1942918	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	2	ALA1938338	cell-based format	Scientific Literature
9.	ALA1942919	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 5 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	4	ALA1938338	cell-based format	Scientific Literature
10.	ALA1943302	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 to 80 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	1	ALA1938338	cell-based format	Scientific Literature
11.	ALA1943303	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as CHOP protein expression at 5 uM after 5 hrs by Western blot analysis	Homo sapiens	4	ALA1938338	cell-based format	Scientific Literature
12.	ALA1943304	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 10 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	4	ALA1938338	cell-based format	Scientific Literature
13.	ALA1943305	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 to 20 uM after 16 hrs by dual luciferase reporter gene assay relative to control	Homo sapiens	4	ALA1938338	cell-based format	Scientific Literature
14.	ALA1943308	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as phosphorylation of eIF2alpha at 5 uM after 5 hrs by Western blot analysis	Homo sapiens	4	ALA1938338	cell-based format	Scientific Literature
15.	ALA1943681	Binding	Activation of EIF2alpha kinase in human CRL2351 cells assessed as CHOP mRNA expression at 5 uM after 5 hrs by PCR analysis	Homo sapiens	4	ALA1938338	cell-based format	Scientific Literature
16.	ALA1040657	Binding	Residual activity of HRI at 0.1 uM by microplate scintillation counting		3	ALA1152560	single protein format	Scientific Literature
17.	ALA1038414	Binding	Residual activity of HRI at 1 uM by microplate scintillation counting		3	ALA1152560	single protein format	Scientific Literature
18.	ALA1046829	Binding	Residual activity of HRI at 10 uM by microplate scintillation counting		3	ALA1152560	single protein format	Scientific Literature
19.	ALA1058424	Binding	Inhibition of human HRI kinase	Homo sapiens	28	ALA1154656	single protein format	Scientific Literature
20.	ALA2172950	Binding	Inhibition of HRI assessed as EIF2AK1 phosphorylation		5	ALA2169840	single protein format	Scientific Literature