# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1058424 | B | Inhibition of human HRI kinase | Homo sapiens | 28 | single protein format | Scientific Literature | ||
2. | ALA1040657 | B | Residual activity of HRI at 0.1 uM by microplate scintillation counting | 3 | single protein format | Scientific Literature | |||
3. | ALA1038414 | B | Residual activity of HRI at 1 uM by microplate scintillation counting | 3 | single protein format | Scientific Literature | |||
4. | ALA1046829 | B | Residual activity of HRI at 10 uM by microplate scintillation counting | 3 | single protein format | Scientific Literature | |||
5. | ALA1174394 | B | Inhibition of EIF2AK1 at 10 uM | 2 | single protein format | Scientific Literature | |||
6. | ALA1908777 | B | Binding constant for EIF2AK1 kinase domain | 72 | single protein format | Scientific Literature | |||
7. | ALA1942915 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 40 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
8. | ALA1942916 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 20 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
9. | ALA1942918 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA1942919 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 5 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
11. | ALA1943302 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 to 80 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
12. | ALA1943303 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as CHOP protein expression at 5 uM after 5 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
13. | ALA1943304 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 10 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
14. | ALA1943305 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 to 20 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
15. | ALA1943308 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as phosphorylation of eIF2alpha at 5 uM after 5 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
16. | ALA1943681 | B | Activation of EIF2alpha kinase in human CRL2351 cells assessed as CHOP mRNA expression at 5 uM after 5 hrs by PCR analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
17. | ALA2172950 | B | Inhibition of HRI assessed as EIF2AK1 phosphorylation | 5 | single protein format | Scientific Literature | |||
18. | ALA2211492 | B | Inhibition of EIF2AK1 | 1 | single protein format | Scientific Literature | |||
19. | ALA2214017 | B | Inhibition of EIF2AK1 assessed as residual activity at 10 uM relative to control | 1 | single protein format | Scientific Literature | |||
20. | ALA2443948 | B | Inhibition of HRI (unknown origin) assessed as EIF2AK1 phosphorylation | Homo sapiens | 3 | single protein format | Scientific Literature |