| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA896564 | B | Binding affinity to human OGT expressed in Escherichia coli BL1 (DE3) cells assessed as alpha-FLAG-HRP activity relative to alpha-A-crystallin peptide by azido-ELISA | Homo sapiens | 28 | ALA1149538 | cell-based format | Scientific Literature | |
| 2. | ALA896565 | B | Binding affinity to human OGT expressed in Escherichia coli BL1 (DE3) cells by azido-ELISA | Homo sapiens | 3 | ALA1149538 | cell-based format | Scientific Literature | |
| 3. | ALA1670863 | B | Activity at human OGT by Western blot analysis | Homo sapiens | 2 | ALA1667854 | single protein format | Scientific Literature | |
| 4. | ALA1670864 | B | Inhibition of human OGT | Homo sapiens | 1 | ALA1667854 | single protein format | Scientific Literature | |
| 5. | ALA3598561 | B | Inhibition of nucleocytoplasmic OGT (unknown origin) | Homo sapiens | 3 | ALA3596159 | single protein format | Scientific Literature | |
| 6. | ALA3598562 | B | Inhibition of OGT (unknown origin) | Homo sapiens | 3 | ALA3596159 | single protein format | Scientific Literature | |
| 7. | ALA3599242 | B | Inhibition of human OGT using [3H]-UDP-GlcNAc as donor and recombinant nup2 as acceptor at 50 uM by liquid scintillation counting analysis | Homo sapiens | 8 | ALA3596110 | single protein format | Scientific Literature | |
| 8. | ALA3599243 | B | Inhibition of human OGT using [3H]-UDP-GlcNAc as donor and recombinant nup2 as acceptor by liquid scintillation counting analysis | Homo sapiens | 6 | ALA3596110 | single protein format | Scientific Literature | |
| 9. | ALA3599244 | B | Inhibition of human OGT using [3H]-UDP-GlcNAc as donor and recombinant nup2 as acceptor by Dixon plot analysis | Homo sapiens | 3 | ALA3596110 | single protein format | Scientific Literature | |
| 10. | ALA3599245 | B | Inhibition of OGT in human MCF7 cells assessed as decrease in intracellular O-GlcNAcylated protein level up to 500 uM after 24 to 48 hrs by immunoblot analysis | Homo sapiens | 7 | ALA3596110 | cell-based format | Scientific Literature | |
| 11. | ALA3599246 | B | Inhibition of OGT in human MCF10A cells assessed as decrease in intracellular O-GlcNAcylated protein level up to 500 uM after 24 to 48 hrs by immunoblot analysis | Homo sapiens | 1 | ALA3596110 | cell-based format | Scientific Literature | |
| 12. | ALA3599247 | B | Inhibition of OGT in human HeLa cells assessed as decrease in intracellular O-GlcNAcylated protein level up to 500 uM after 24 to 48 hrs by immunoblot analysis | Homo sapiens | 1 | ALA3596110 | cell-based format | Scientific Literature | |
| 13. | ALA3705855 | B | Inhibition Assay: 384-well plates (Costar #3654) were filled using a liquid handling robot with 20 uL of a mixture of 50 nM of a fluorescein-linked UDP-GlcNAc analog (see Gross et al, 2003), 1-2 uM sOGT, and buffer (20 mM potassium phosphate, pH=7.4 with 500 uM tris(hydroxypropyl)phosphine). The 1249 compound library was serially diluted in DMSO from the 5 mg/ml plates fivefold 3 times, such that 4 different concentrations of compounds were prepared. Compound libraries of the 4 concentrations in duplicated were then transferred to the assay plates using a 100 nL pin array, resulting in a final compound concentration of 25 ug/mL or 70 uM at the highest of the four concentrations, assuming an average compound MW of 350. Using a Perkin Elmer Envision microplate reader, the sample was excited at 480 nm in the vertical plane, and simultaneous emission intensity (535 nm) of the vertical and horizontal polarization planes was measured. | 26 | ALA3639323 | single protein format | BindingDB Database | ||
| 14. | ALA3864091 | B | Binding affinity to OGT (unknown origin) assessed as tethering of cysteine residues at 4 mM at 37 degC for 2 hrs in presence of Tris-HCL buffer containing 1 mM DTT by SDS-PAGE method | Homo sapiens | 1 | ALA3862044 | single protein format | Scientific Literature | |
| 15. | ALA3864092 | B | Binding affinity to OGT (unknown origin) assessed as tethering of Cys921 and Cys927 residues at 4 mM at 37 degC for 2 hrs in presence of Tris-HCL buffer containing 1 mM DTT by SDS-PAGE method | Homo sapiens | 1 | ALA3862044 | single protein format | Scientific Literature | |
| 16. | ALA3864093 | B | Inhibition of OGT (unknown origin) assessed as reduction in GlcNAc transfer to CKII peptide at 100 uM incubated for 60 mins by HPLC method | Homo sapiens | 2 | ALA3862044 | single protein format | Scientific Literature | |
| 17. | ALA3864094 | B | Binding affinity to OGT (unknown origin) assessed as modification at Cys590 residues following in-gel trypsin digestion by LC/MS/MS method | Homo sapiens | 13 | ALA3862044 | single protein format | Scientific Literature | |
| 18. | ALA3864095 | B | Binding affinity to OGT (unknown origin) assessed as modification at Cys531/Cys620 residues following in-gel trypsin digestion by LC/MS/MS method | Homo sapiens | 13 | ALA3862044 | single protein format | Scientific Literature | |
| 19. | ALA3864096 | B | Inhibition of OGT (unknown origin) assessed as reduction in GlcNAc transfer to CKII peptide at 1 mM incubated for 60 mins by HPLC method based inactivation time course analysis | Homo sapiens | 3 | ALA3862044 | single protein format | Scientific Literature | |
| 20. | ALA3864097 | B | Inhibition of OGT (unknown origin) assessed as reduction in GlcNAc transfer to CKII peptide incubated for 60 mins by HPLC method | Homo sapiens | 13 | ALA3862044 | single protein format | Scientific Literature |
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