| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA886349 | B | Inhibition of isopeptidase T at 40 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 2. | ALA1243705 | B | Inhibition of isopeptidase T | 1 | single protein format | Scientific Literature | |||
| 3. | ALA2163013 | B | Inhibition of USP5 by Ub-CHOP reporter assay | 1 | single protein format | Scientific Literature | |||
| 4. | ALA2411908 | B | Inhibition of USP5 in human Z138 cells after 1 hr by immunoblotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA2411910 | B | Inhibition of human USP5 using Ub4 as substrate at 50 uM after 10 mins by SDS-PAGE analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 6. | ALA2411915 | B | Inhibition of human USP5 using Ub-AMC as substrate at 1 to 20 uM after 10 mins by fluorometric analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA2411922 | B | Competitive inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA2411924 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate after 60 mins by fluorometric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA2412073 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 5 to 50 uM after 15 mins by SDS-PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA2412074 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 20 uM after 15 mins by SDS-PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA2412075 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 10 uM after 15 mins by SDS-PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA2412076 | B | Competitive binding affinity to human USP5 C335A/H818A mutant by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA2412077 | B | Competitive binding affinity to human USP5 H818A mutant by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA2412078 | B | Competitive binding affinity to human USP5 C335A mutant by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA2412079 | B | Competitive binding affinity to human recombinant USP5 after 1 hr by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 16. | ALA2412081 | B | Binding affinity to human recombinant USP5 by SDS-PAGE analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 17. | ALA4118711 | B | Binding affinity to USP5 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA4119763 | B | Inhibition of USP5 (unknown origin) using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorescence based assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 19. | ALA4257362 | B | Inhibition of USP5 in human SK-MEL-28 cells by LC/MS/MS analysis | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
| 20. | ALA4257363 | B | Inhibition of USP5 in human A375 cells by LC/MS/MS analysis | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data |
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