#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA886349	Binding	Inhibition of isopeptidase T at 40 uM	Homo sapiens	1	ALA1139949	single protein format	Scientific Literature
2.	ALA1243705	Binding	Inhibition of isopeptidase T		1	ALA1240332	single protein format	Scientific Literature
3.	ALA4358860	Binding	Inhibition of human recombinant isopeptidase T assessed as reduction in cleavage of luminescent substrate Ub-AML at 100 uM	Homo sapiens	1	ALA4354861	single protein format	Scientific Literature
4.	ALA2163013	Binding	Inhibition of USP5 by Ub-CHOP reporter assay		1	ALA2157895	single protein format	Scientific Literature
5.	ALA2411908	Binding	Inhibition of USP5 in human Z138 cells after 1 hr by immunoblotting analysis	Homo sapiens	1	ALA2406999	cell-based format	Scientific Literature
6.	ALA2411910	Binding	Inhibition of human USP5 using Ub4 as substrate at 50 uM after 10 mins by SDS-PAGE analysis	Homo sapiens	3	ALA2406999	single protein format	Scientific Literature
7.	ALA2411915	Binding	Inhibition of human USP5 using Ub-AMC as substrate at 1 to 20 uM after 10 mins by fluorometric analysis	Homo sapiens	1	ALA2406999	single protein format	Scientific Literature
8.	ALA2411922	Binding	Competitive inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
9.	ALA2411924	Binding	Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate after 60 mins by fluorometric analysis	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
10.	ALA2412073	Binding	Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 5 to 50 uM after 15 mins by SDS-PAGE analysis	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
11.	ALA2412074	Binding	Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 20 uM after 15 mins by SDS-PAGE analysis	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
12.	ALA2412075	Binding	Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 10 uM after 15 mins by SDS-PAGE analysis	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
13.	ALA2412076	Binding	Competitive binding affinity to human USP5 C335A/H818A mutant by SDS-PAGE analysis in presence of biotin-DMT	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
14.	ALA2412077	Binding	Competitive binding affinity to human USP5 H818A mutant by SDS-PAGE analysis in presence of biotin-DMT	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
15.	ALA2412078	Binding	Competitive binding affinity to human USP5 C335A mutant by SDS-PAGE analysis in presence of biotin-DMT	Homo sapiens	1	ALA2406999	assay format	Scientific Literature
16.	ALA2412079	Binding	Competitive binding affinity to human recombinant USP5 after 1 hr by SDS-PAGE analysis in presence of biotin-DMT	Homo sapiens	2	ALA2406999	single protein format	Scientific Literature
17.	ALA2412081	Binding	Binding affinity to human recombinant USP5 by SDS-PAGE analysis	Homo sapiens	2	ALA2406999	single protein format	Scientific Literature
18.	ALA4118711	Binding	Binding affinity to USP5 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay	Homo sapiens	1	ALA4118021	cell-based format	Scientific Literature
19.	ALA4119763	Binding	Inhibition of USP5 (unknown origin) using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorescence based assay	Homo sapiens	1	ALA4118084	single protein format	Scientific Literature
20.	ALA4257362	Binding	Inhibition of USP5 in human SK-MEL-28 cells by LC/MS/MS analysis	Homo sapiens	1	ALA4257227	cell-based format	Patent Bioactivity Data