# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA886349 | B | Inhibition of isopeptidase T at 40 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
2. | ALA1243705 | B | Inhibition of isopeptidase T | 1 | single protein format | Scientific Literature | |||
3. | ALA2163013 | B | Inhibition of USP5 by Ub-CHOP reporter assay | 1 | single protein format | Scientific Literature | |||
4. | ALA5251113 | B | Inhibition of human USP5 (1 to 835 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA5251138 | B | Inhibition of human USP5 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
6. | ALA5251146 | B | Inhibition of USP5 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
7. | ALA5251147 | B | Inhibition of USP5 (unknown origin) using ubiquitin rhodamine 110 as substrate in presence of ubiquitin at Kd at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
8. | ALA5251148 | B | Inhibition of USP5 (unknown origin) using ubiquitin rhodamine 110 as substrate in presence of ubiquitin at binding maximum at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
9. | ALA2411908 | B | Inhibition of USP5 in human Z138 cells after 1 hr by immunoblotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
10. | ALA2411910 | B | Inhibition of human USP5 using Ub4 as substrate at 50 uM after 10 mins by SDS-PAGE analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
11. | ALA2411915 | B | Inhibition of human USP5 using Ub-AMC as substrate at 1 to 20 uM after 10 mins by fluorometric analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
12. | ALA2411922 | B | Competitive inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate | Homo sapiens | 1 | assay format | Scientific Literature | ||
13. | ALA2411924 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate after 60 mins by fluorometric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
14. | ALA2412073 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 5 to 50 uM after 15 mins by SDS-PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
15. | ALA2412074 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 20 uM after 15 mins by SDS-PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
16. | ALA2412075 | B | Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using K48-linked tetraubiquitin as substrate assessed as substrate hydrolysis at 10 uM after 15 mins by SDS-PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
17. | ALA2412076 | B | Competitive binding affinity to human USP5 C335A/H818A mutant by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 1 | assay format | Scientific Literature | ||
18. | ALA2412077 | B | Competitive binding affinity to human USP5 H818A mutant by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 1 | assay format | Scientific Literature | ||
19. | ALA2412078 | B | Competitive binding affinity to human USP5 C335A mutant by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 1 | assay format | Scientific Literature | ||
20. | ALA2412079 | B | Competitive binding affinity to human recombinant USP5 after 1 hr by SDS-PAGE analysis in presence of biotin-DMT | Homo sapiens | 2 | single protein format | Scientific Literature |