# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1037349 | B | Inhibition of Leishmania major recombinant PTR1 | Leishmania major | 19 | single protein format | Scientific Literature | ||
2. | ALA1655366 | B | Inhibition of Leishmania major PTR1 | Leishmania major | 18 | single protein format | Scientific Literature | ||
3. | ALA1655367 | B | Inhibition of Leishmania major PTR1 by Lineweaver-Burk analysis | Leishmania major | 13 | single protein format | Scientific Literature | ||
4. | ALA1655379 | B | Inhibition of Leishmania major PTR1 at 500 uM | Leishmania major | 47 | single protein format | Scientific Literature | ||
5. | ALA1655390 | B | Inhibition of Leishmania major PTR1 at 5 uM | Leishmania major | 29 | single protein format | Scientific Literature | ||
6. | ALA2183542 | B | Inhibition of pteridine reductase in Leishmania major promastigote assessed as reduction in cell survival upon H2O2 exposure at 100 ug/mL after 48 hrs relative to control | Leishmania major | 2 | single protein format | Scientific Literature | ||
7. | ALA2183545 | B | Inhibition of pteridine reductase in Leishmania major promastigote assessed as increase in pyrimethamine cytotoxicity by Chou-Talalay method | Leishmania major | 2 | single protein format | Scientific Literature | ||
8. | ALA2183549 | B | Inhibition of pteridine reductase in Leishmania major promastigotes assessed as increase in pyrimethamine-induced growth inhibition by measuring parasite growth at 50 ug/mL | Leishmania major | 6 | single protein format | Scientific Literature | ||
9. | ALA2185822 | B | Inhibition of Leishmania major PTR1 by spectrophotometric assay | Leishmania | 10 | single protein format | Scientific Literature | ||
10. | ALA3412013 | B | Binding affinity to Leishmania major pteridine reductase 1 | Leishmania major | 6 | single protein format | Scientific Literature | ||
11. | ALA3872971 | B | Binding affinity to Leishmania major recombinant N-terminal hexa-His tagged PTR1 expressed in Escherichia coli BL21(DE3) assessed as change in melting temperature at 50 uM at pH 4.7 by SYPRO orange dye based thermal shift assay | Leishmania major | 7 | assay format | Scientific Literature | ||
12. | ALA3872972 | B | Inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) at 50 uM using biopterin as substrate measured for 60 sec in presence of NADPH by UV-visible spectrophotometric method relative to control | Leishmania major | 3 | assay format | Scientific Literature | ||
13. | ALA3872973 | B | Inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) using biopterin as substrate measured for 60 sec in presence of NADPH by UV-visible spectrophotometric method relative to control | Leishmania major | 3 | assay format | Scientific Literature | ||
14. | ALA3872974 | B | Competitive inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) assessed as decrease in thermal shift stabilization at 50 uM at pH 4.7 in presence of varying levels of biopterin and constant NADPH level by SYPRO orange dye based assay | Leishmania major | 1 | assay format | Scientific Literature | ||
15. | ALA3872975 | B | Competitive inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) at 25 to 45 uM at pH 4.7 in presence of saturating concentration of NADPH and varying levels of biopterin by double reciprocal plot analysis | Leishmania major | 1 | assay format | Scientific Literature | ||
16. | ALA3872976 | B | Noncompetitive inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) at 25 to 45 uM at pH 4.7 in presence of saturating concentration of biopterin and varying levels of NADPH by double-reciprocal plot analysis | Leishmania major | 1 | assay format | Scientific Literature | ||
17. | ALA3872977 | B | Effect on thermal stability of Leishmania major recombinant N-terminal hexa-His tagged PTR1 expressed in Escherichia coli BL21(DE3) by SYPRO orange dye based thermal shift assay | Leishmania major | 2 | assay format | Scientific Literature | ||
18. | ALA3872978 | B | Noncompetitive inhibition of Leishmania major recombinant N-terminal hexa-His tagged PTR1 catalytic activity expressed in Escherichia coli BL21(DE3) assessed as decrease in thermal shift stabilization at 50 uM at pH 4.7 in presence of varying levels of NADPH and constant biopterin substrate concentration by SYPRO orange dye based assay | Leishmania major | 1 | assay format | Scientific Literature | ||
19. | ALA4026061 | B | Binding affinity to Leishmania major PTR1 BL21(DE3) assessed as change in melting temperature at 50 uM by SYPRO orange dye based RT-PCR analysis | Leishmania major | 2 | single protein format | Scientific Literature | ||
20. | ALA4026062 | B | Binding affinity to Leishmania major PTR1 BL21(DE3) assessed as change in melting temperature by SYPRO orange dye based RT-PCR analysis | Leishmania major | 2 | single protein format | Scientific Literature |