# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3816043 | Binding | Agonist activity at mouse GPR34 expressed in HEK293A cells cotransfected with chimeric Galphaq/il after 1.5 hrs by alkaline phosphatase tagged-TGFalpha shedding assay | Mus musculus | 36 | ALA3813596 | cell-based format | Scientific Literature | |
2. | ALA3816045 | Binding | Intrinsic activity at mouse GPR34 expressed in HEK293A cells cotransfected with chimeric Galphaq/il after 1.5 hrs by alkaline phosphatase tagged-TGFalpha shedding assay relative to O-(Hydroxy(((2R,3R)-2-(((3-(2-((3-phenoxybenzyl)oxy)phenyl)-propanoyl)oxy)methyl)tetrahydro-2H-pyran-3-yl)oxy)phosphoryl)-L-serine | Mus musculus | 35 | ALA3813596 | cell-based format | Scientific Literature | |
3. | ALA3816048 | Functional | Agonist activity at mouse GPR34 expressed in HEK293A cells cotransfected with chimeric Galphaq/il assessed as increase in intracellular calcium levels at 1 uM measured after 35 secs by calcium 5 dye based fluorescence microplate reader method in presence of probenecid | Mus musculus | 6 | ALA3813596 | cell-based format | Scientific Literature | |
4. | ALA1048043 | Functional | Activation of mouse GPR34 expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production at 0.01 to 10 uM after 30 mins by EIA | Mus musculus | 2 | ALA1156051 | cell-based format | Scientific Literature | |
5. | ALA1048044 | Functional | Activation of mouse GPR34 expressed in CHOK1 cells assessed as increase in intracellular calcium level by spectrofluorimetry | Mus musculus | 2 | ALA1156051 | cell-based format | Scientific Literature | |
6. | ALA1048046 | Binding | Activation of mouse GPR34 expressed in CHOK1 cells assessed as phosphorylated ERK level at 0.01 to 10 uM by Western blotting | Mus musculus | 2 | ALA1156051 | cell-based format | Scientific Literature | |
7. | ALA1048048 | Binding | Activation of mouse GPR34 expressed in CHOK1 cells assessed as total ERK level at 0.01 to 10 uM by Western blotting | Mus musculus | 2 | ALA1156051 | cell-based format | Scientific Literature | |
8. | ALA4014236 | Binding | Agonist activity at mouse GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/chimeric Galphaq/i1 assessed as induction of ectodomain shedding of membrane bound AP-TGFalpha after 1 hr | Mus musculus | 36 | ALA4011662 | cell-based format | Scientific Literature | |
9. | ALA4014237 | Binding | Intrinsic activity at mouse GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/chimeric Galphaq/iL assessed as induction of ectodomain shedding of membrane bound AP-TGFalpha after 1 hr relative to O-(Hydroxy(((2R,3R)-2-(((3-(2-((3-phenoxybenzyl)oxy)phenyl)- propanoyl)oxy)methyl)tetrahydro-2H-pyran-3-yl)oxy)phosphoryl)- L-serine | Mus musculus | 30 | ALA4011662 | cell-based format | Scientific Literature | |
10. | ALA4014240 | Binding | Agonist activity at mouse GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/chimeric Galphaq/i1 assessed as induction of ectodomain shedding of membrane bound AP-TGFalpha after 1 hr relative to control | Mus musculus | 30 | ALA4011662 | cell-based format | Scientific Literature | |
11. | ALA4729127 | Binding | Agonist activity at mouse GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/Galphaq/i1 assessed as induction of ectodomain shedding of membrane bound AP-TGFalpha after 1 hr by p-NPP substrate based microplate reader analysis | Mus musculus | 30 | ALA4725358 | cell-based format | Scientific Literature | |
12. | ALA4729128 | Binding | Agonist activity at mouse GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/Galphaq/i1 assessed as induction of ectodomain shedding of membrane bound AP-TGFalpha after 1 hr by p-NPP substrate based microplate reader analysis relative to control | Mus musculus | 30 | ALA4725358 | cell-based format | Scientific Literature | |
13. | ALA4729129 | Binding | Agonist activity at mouse GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/Galphaq/i1 assessed as induction of ectodomain shedding of membrane bound AP-TGFalpha after 1 hr by p-NPP substrate based microplate reader analysis relative to positive control | Mus musculus | 30 | ALA4725358 | cell-based format | Scientific Literature | |
14. | ALA4729133 | Functional | Agonist activity at mouse GPR34 expressed in HEK293 cells co-transfected with Galphaq/i1 assessed as increase in calcium influx by calcium 5 dye based FLIPR assay | Mus musculus | 2 | ALA4725358 | cell-based format | Scientific Literature |