#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA901259	B	Inhibition of mouse Sphk2 expressed in HEK293T cells	Mus musculus	4	ALA1138321	cell-based format	Scientific Literature
2.	ALA1110617	B	Induction of mouse SphK2 phosphorylation	Mus musculus	3	ALA1156285	single protein format	Scientific Literature
3.	ALA1111695	B	Inhibition of mouse recombinant SPHK2 expressed in baculovirus-infected Sf9 cells in presence of sphingosine	Mus musculus	14	ALA1156306	cell-based format	Scientific Literature
4.	ALA1111706	B	Ratio of Kinact to Km for mouse recombinant SPHK2 expressed in baculovirus-infected Sf9 cells	Mus musculus	14	ALA1156306	cell-based format	Scientific Literature
5.	ALA1777256	B	Inhibition of mouse recombinant SPHK2 expressed in baculovirus-infected Sf9 cells	Mus musculus	28	ALA1772987	cell-based format	Scientific Literature
6.	ALA1929793	B	Inhibition of mouse SphK2 expressed in baculovirus infected Sf9 insect cells assessed as remaining activity using sphingosine and [gamma-(32)P]-ATP at 100 uM by scintillation proximity counting	Mus musculus	41	ALA1926562	cell-based format	Scientific Literature
7.	ALA1929794	B	Inhibition of mouse SphK2 expressed in baculovirus infected Sf9 insect cells assessed as remaining activity using sphingosine and [gamma-(32)P]-ATP at 10 uM by scintillation proximity counting	Mus musculus	3	ALA1926562	cell-based format	Scientific Literature
8.	ALA1929796	B	Inhibition of mouse SphK2 expressed in baculovirus infected Sf9 insect cells using sphingosine and [gamma-(32)P]-ATP by scintillation proximity assay	Mus musculus	8	ALA1926562	cell-based format	Scientific Literature
9.	ALA1929802	B	Inhibition of mouse SphK2 expressed in baculovirus infected Sf9 insect cells using sphingosine and [gamma-(32)P]-ATP at 10 uM by scintillation proximity counting	Mus musculus	4	ALA1926562	cell-based format	Scientific Literature
10.	ALA2404692	B	Activity at mouse SPHK2 transfected in mouse A20/2J cell lysates assessed as compound phosphorylation at 10 uM after 30 mins by autoradiography in presence of [gamma-32P]ATP	Mus musculus	2	ALA2401642	cell-based format	Scientific Literature
11.	ALA2404704	B	Inhibition of mouse SPHK2 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 2 to 4 uM incubated for 5 mins prior to substrate addition measured after 5 mins	Mus musculus	2	ALA2401642	cell-based format	Scientific Literature
12.	ALA2404706	B	Inhibition of mouse SPHK2 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 8 uM incubated for 5 mins prior to substrate addition measured after 5 mins	Mus musculus	1	ALA2401642	cell-based format	Scientific Literature
13.	ALA2412212	B	Inhibition of mouse SphK2	Mus musculus	2	ALA2406911	single protein format	Scientific Literature
14.	ALA3106151	A	Prodrug conversion assessed as mouse SphK2-mediated formation of phosphorylated compound	Mus musculus	17	ALA3102761	single protein format	Scientific Literature
15.	ALA3630967	B	Inhibition of mouse recombinant SphK2 assessed as remaining enzyme activity at 10 uM using gamma[32P]ATP incubated for 20 mins by liquid scintillation counting method	Mus musculus	21	ALA3627716	single protein format	Scientific Literature
16.	ALA3630969	B	Inhibition of mouse recombinant SphK2 assessed as remaining enzyme activity at 1 uM using gamma[32P]ATP incubated for 20 mins by liquid scintillation counting method	Mus musculus	7	ALA3627716	single protein format	Scientific Literature
17.	ALA3888897	B	Kinase Assay: Sphingosine kinase activity was assessed as described previously (Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Setiady, Y., Zhu, R., Zigler, M. A., Burcin, T. L., Ley, K., Tung, K. S. K., Engelhard, V. H., Macdonald, T. L. and Lynch, K. R. Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720. J Biological Chemistry 280: 36865-36872 (2005)). High levels of human sphingosine kinase type 1 (mSK1) and mouse sphingosine kinase type 2 (mSK2) were expressed in Sf9 insect cells by infection with cognate baculoviruses. Crude homogenates were incubated with γ-[32P]ATP and 10 micromolar D-erythro-sphingosine in the presence of 100 micromolar concentrations of test compounds for 20 minutes at 37 C. The product, radiolabeled S1P, was isolated by extraction into organic solvents after acidification and displaced by thin layer chromatography.		36	ALA3886879	cell-based format	BindingDB Database
18.	ALA4034232	B	Inhibition of recombinant mouse SphK2 expressed in baculovirus infected sf9 cells using D-erythro-sphingosine as substrate in presence of [gamma-33P]ATP by scintillation counting method	Mus musculus	1	ALA4033636	cell-based format	Scientific Literature
19.	ALA4051842	B	Inhibition of recombinant mouse SPHK2 using sphingosine as substrate in presence ATP	Mus musculus	2	ALA4049441	single protein format	Scientific Literature
20.	ALA4357632	B	Inhibition of recombinant mouse SphK2 expressed in baculovirus infected Sf9 insect cells using D-erythro-sphingosine as substrate measured after 30 mins in presence of [gamma32P]-ATP by liquid scintillation counting method	Mus musculus	1	ALA4354840	cell-based format	Scientific Literature