# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA905732 | B | Inhibition of Mycobacterium tuberculosis DHFR expressed in Saccharomyces cerevisiae TB5 assessed as yeast growth inhibition after 3 days by radial spoke assay | Mycobacterium tuberculosis | 19 | ALA1143059 | assay format | Scientific Literature | |
2. | ALA1677337 | B | Inhibition of Mycobacterium tuberculosis dihydrofolate reductase | Mycobacterium tuberculosis | 1 | ALA1671707 | single protein format | Scientific Literature | |
3. | ALA3755621 | B | Inhibition of recombinant Mycobacterium tuberculosis DHFR using 45 uM DHF as substrate by spectrophotometry | Mycobacterium tuberculosis | 2 | ALA3751663 | single protein format | Scientific Literature | |
4. | ALA4146152 | B | Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli BL21(DE3) assessed as reduction in consumption of NADPH using DHF as substrate preincubated for 1 min followed by DHF addition and measured for 2 mins by spectrophotometric method | Mycobacterium tuberculosis H37Rv | 2 | ALA4145451 | assay format | Scientific Literature | |
5. | ALA4146154 | B | Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli assessed as reduction in consumption of NADPH using DHF as substrate by fluorescence method | Mycobacterium tuberculosis H37Rv | 3 | ALA4145451 | assay format | Scientific Literature | |
6. | ALA4146157 | B | Inhibition of Mycobacterium tuberculosis H37Rv DHFR assessed as reduction in consumption of NADPH using DHF as substrate incubated for 3 mins measured for 2 mins by spectrophotometric method | Mycobacterium tuberculosis H37Rv | 2 | ALA4145451 | single protein format | Scientific Literature | |
7. | ALA4146166 | B | Inhibition of Mycobacterium tuberculosis His-tagged DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs over 6 mins by spectrophotometric method | Mycobacterium tuberculosis | 5 | ALA4145451 | single protein format | Scientific Literature | |
8. | ALA4368148 | B | Inhibition of Mycobacterium tuberculosis H37Rv DHFR | Mycobacterium tuberculosis H37Rv | 1 | ALA4364320 | single protein format | Scientific Literature | |
9. | ALA4481836 | B | Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli BL21(DE3) assessed as reduction in consumption of NADPH using DHF as substrate preincubated for 1 min followed by DHF addition and measured for 2 mins by spectrophotometric method | Mycobacterium tuberculosis H37Rv | 4 | ALA4480432 | assay format | Scientific Literature | |
10. | ALA4625598 | B | Inhibition of recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis | Mycobacterium tuberculosis | 22 | ALA4622960 | cell-based format | Scientific Literature | |
11. | ALA4625599 | B | Uncompetitive inhibition of Mycobacterium tuberculosis DHFR assessed as constant Km using DHF as substrate and NADPH as cofactor by Michaelis-Menten analysis | Mycobacterium tuberculosis | 1 | ALA4622960 | single protein format | Scientific Literature | |
12. | ALA4625600 | B | Uncompetitive inhibition of Mycobacterium tuberculosis DHFR assessed as varying Vmax using DHF as substrate and NADPH as cofactor by Michaelis-Menten analysis | Mycobacterium tuberculosis | 1 | ALA4622960 | single protein format | Scientific Literature | |
13. | ALA4625601 | B | Potency index, ratio of MB872 IC50 to test compound IC50 for recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis | Mycobacterium tuberculosis | 6 | ALA4622960 | cell-based format | Scientific Literature | |
14. | ALA4625602 | B | Potency index, ratio of trimethoprim IC50 to test compound IC50 for recombinant Mycobacterium tuberculosis DHFR expressed in Escherichia coli BL21(DE3) cells using DHF as substrate and NADPH as cofactor by fluorimetric analysis | Mycobacterium tuberculosis | 1 | ALA4622960 | cell-based format | Scientific Literature | |
15. | ALA5234352 | B | Inhibition of Mycobacterium tuberculosis DHFR | Mycobacterium tuberculosis | 8 | ALA5233242 | single protein format | Scientific Literature | |
16. | ALA5234358 | B | Inhibition of recombinant Mycobacterium tuberculosis DHFR using dihydrofolate as substrate preincubated for 1 min followed by substrate addition and further incubated for 2 mins in presence of NADPH by spectrophotometer | Mycobacterium tuberculosis | 1 | ALA5233242 | single protein format | Scientific Literature |