# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1111551 | Functional | Antagonist activity at ML-IAP MLXBIR3SG domain by fluorescence polarization assay | 35 | ALA1156287 | assay format | Scientific Literature | ||
2. | ALA2066538 | Binding | Binding affinity to ML-IAP BIR domain after 30 mins by fluorescence polarization-based competition assay | Homo sapiens | 1 | ALA2062482 | single protein format | Scientific Literature | |
3. | ALA2066550 | Binding | Inhibition of ML-IAP binding to Smax expressed in gemcitabine and zVAd treated human SK-MEL28 cells at 0.5 uM by immunoprecipitation | Homo sapiens | 1 | ALA2062482 | cell-based format | Scientific Literature | |
4. | ALA4351163 | Binding | Binding affinity to ML-IAP-BIR domain (unknown origin) | Homo sapiens | 2 | ALA4350953 | single protein format | Scientific Literature | |
5. | ALA5098726 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
6. | ALA5098727 | Binding | Antagonist activity at N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
7. | ALA5098731 | Binding | Antagonist activity at N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
8. | ALA5098735 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues)(unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:2 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
9. | ALA5098737 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:10 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
10. | ALA5098740 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as inhibition constant using AVPIAQKSEK-biotinylated peptide as substrate incubated upto 40 mins by DELFIA assay | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
11. | ALA5098741 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as inactivation constant using AVPIAQKSEK-biotinylated peptide as substrate incubated upto 40 mins by DELFIA assay | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
12. | ALA5098743 | Binding | Binding affinity to ML-IAP in human SK-MEL-28 cells assessed as change in melting temperature at 10 uM incubated for 3 hrs followed by heating at 37 to 70 degree C for 3 mins by CETSA analysis (Rvb = 50 degreeC) | Homo sapiens | 3 | ALA5096205 | cell-based format | Scientific Literature |