| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2039748 | B | Inhibition of human Tdp1 using 3'-phosphate-(4-methylumbelliferone)-thymidine as substrate after 180 mins by spectrophotometry | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 2. | ALA2061432 | B | Inhibition of human recombinant Tdp1 using 5'-[32P]-labeled single-stranded DNA oligonucleotide containing 3'-phosphotyrosine as substrate assessed as prevention of generation of oligonucleotide with free 3'-phosphate after 15 mins by PAGE analysis | Homo sapiens | 76 | single protein format | Scientific Literature | ||
| 3. | ALA2061433 | B | Inhibition of human Tdp1 in Tdp1-deficient chicken DT40 whole cell extract using 5'-[32P]-labeled single-stranded DNA oligonucleotide containing 3'-phosphotyrosine as substrate after 15 mins by PAGE analysis | Homo sapiens | 12 | single protein format | Scientific Literature | ||
| 4. | ALA2061434 | B | Binding affinity to human recombinant Tdp1 at 0.4 to 33 uM by surface plasmon resonance spectroscopy analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA2061435 | B | Competitive inhibition of human recombinant Tdp1 after 1 hr by FRET assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA2061436 | B | Inhibition of Tdp1 | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 7. | ALA1068068 | B | Inhibition of human tyrosyl-DNA phosphodiesterase 1 after 30 mins by alpha high throughput screening assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 8. | ALA1068069 | B | Inhibition of human tyrosyl-DNA phosphodiesterase 1 after 30 mins by alpha high throughput screening assay in presence of 0.01% Tween-20 | Homo sapiens | 8 | single protein format | Scientific Literature | ||
| 9. | ALA1068070 | B | Inhibition of human tyrosyl-DNA phosphodiesterase 1 after 30 mins by alpha high throughput screening assay in presence of 0.05% Tween-20 | Homo sapiens | 8 | single protein format | Scientific Literature | ||
| 10. | ALA1109820 | B | Ratio of IC50 of test compound to IC50 of NSC88915 for human Tdp1 assessed as blockade of enzyme-mediated hydrolysis of phosphodiester linkage between tyrosine and 3^-end of N14Y DNA substrate by SDS-PAGE electrophoresis | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 11. | ALA1109821 | B | Inhibition of human Tdp1 expressed in Escherichia coli assessed as blockade of enzyme-mediated hydrolysis of phosphodiester linkage between tyrosine and 3^-end of N14Y DNA substrate at 1 mM by SDS-PAGE electrophoresis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA1109824 | B | Inhibition of human Tdp1 expressed in Escherichia coli assessed as blockade of enzyme-mediated hydrolysis of phosphodiester linkage between tyrosine and 3^-end of single stranded N14Y DNA substrate by SDS-PAGE electrophoresis | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 13. | ALA1109825 | B | Inhibition of human Tdp1 expressed in Escherichia coli assessed as blockade of enzyme-mediated hydrolysis of phosphodiester linkage between tyrosine and double stranded DNA substrate bearing blunt end by SDS-PAGE electrophoresis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA1109826 | B | Inhibition of human Tdp1 expressed in Escherichia coli assessed as blockade of enzyme-mediated hydrolysis of phosphodiester linkage between tyrosine and double stranded DNA substrate bearing 5^-overhang end by SDS-PAGE electrophoresis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA1109827 | B | Binding affinity to human Tdp1 amine-coupled to sensor chip assessed as increase in resonance units by SPR | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 16. | ALA1109828 | B | Inhibition of human Tdp1 H493R mutant assessed as decrease in enzyme-DNA complexes by DNA sequencing method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA1109829 | B | Inhibition of human Tdp1 H493R mutant assessed as decrease in enzyme-DNA complexes by SDS-PAGE | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 18. | ALA1614364 | B | PUBCHEM_BIOASSAY: qHTS Assay for Inhibitors of Tyrosyl-DNA Phosphodiesterase (TDP1). (Class of assay: confirmatory) | Homo sapiens | 11,958 | single protein format | PubChem BioAssays | ||
| 19. | ALA1738641 | F | PUBCHEM_BIOASSAY: Gel-Based Assay for Inhibitors of Tyrosyl-DNA Phosphodiesterase (TDP1): Hit Validation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID485290, AID485312, AID489007] | Homo sapiens | 2 | assay format | PubChem BioAssays | ||
| 20. | ALA1738394 | F | PUBCHEM_BIOASSAY: Confirmation Assay for Inhibitors of Tyrosyl-DNA Phosphodiesterase (TDP1). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID485290, AID485312, AID504464, AID504474] | Homo sapiens | 662 | assay format | PubChem BioAssays |
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