# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3706302 | Binding | ADP Glo Assay: The ATPase assay is performed according the following protocol: Purified enzyme (20 nM p97), substrate (20 uM ATP) and a dose titration of compounds are mixed in buffer (50 mM TRIS pH 7.5, 20 mM MgCl2, 0.02% TX-100, 1 mM DTT, 0.2% (v/v) glycerol) and incubated at 37 C. for 15 minutes. The reaction is terminated and the level of product generated is measured using the ADP Glo Assay Kit (Promega, Madison Wis.). | 88 | ALA3638398 | single protein format | BindingDB Database | ||
2. | ALA3749463 | Binding | Inhibition of human p97 ATPase incubated for 15 mins by ADP Glo luminescence assay | Homo sapiens | 64 | ALA3745611 | single protein format | Scientific Literature | |
3. | ALA3749465 | Binding | Inhibition of p97 ATPase in human A549 cells assessed as accumulation of K48 poly-ubiquitinated proteins incubated for 6 hrs by immunofluorescence assay | Homo sapiens | 6 | ALA3745611 | cell-based format | Scientific Literature | |
4. | ALA3749466 | Binding | Inhibition of p97 ATPase in human A549 cells assessed as accumulation of CHOP poly-ubiquitinated proteins incubated for 6 hrs by immunofluorescence assay | Homo sapiens | 6 | ALA3745611 | cell-based format | Scientific Literature | |
5. | ALA3749467 | Binding | Inhibition of p97 ATPase in human A549 cells assessed as reduction in p62 protein incubated for 6 hrs by immunofluorescence assay | Homo sapiens | 6 | ALA3745611 | cell-based format | Scientific Literature | |
6. | ALA3749471 | Binding | Inhibition of human p97 ATPase domain1 at 10 uM incubated for 15 mins by ADP Glo luminescence assay | Homo sapiens | 2 | ALA3745611 | single protein format | Scientific Literature | |
7. | ALA3749472 | Binding | Inhibition of human p97 ATPase domain2 at 10 uM incubated for 15 mins by ADP Glo luminescence assay | Homo sapiens | 2 | ALA3745611 | single protein format | Scientific Literature | |
8. | ALA3749132 | Binding | Inhibition of p97 ATPase (unknown origin) | Homo sapiens | 1 | ALA3745611 | single protein format | Scientific Literature | |
9. | ALA3789455 | Binding | Inhibition of wild type human AAA ATPase p97 expressed in Escherichia coli measured by ADPGlo assay in presence of 100 uM ATP | Homo sapiens | 6 | ALA3785079 | assay format | Scientific Literature | |
10. | ALA3802384 | Binding | Inhibition of p97 (unknown origin) ATPase activity after 10 mins by malachite green dye based visible spectrophotometry | Homo sapiens | 1 | ALA3797126 | single protein format | Scientific Literature | |
11. | ALA3802385 | Binding | Inhibition of p97 cysteine-less mutant (unknown origin) at 200 uM after 10 mins by malachite green dye based visible spectrophotometry | Homo sapiens | 1 | ALA3797126 | single protein format | Scientific Literature | |
12. | ALA3802386 | Binding | Inhibition of p97-C209A mutant (unknown origin) after 10 mins by malachite green dye based visible spectrophotometry | Homo sapiens | 1 | ALA3797126 | assay format | Scientific Literature | |
13. | ALA3802592 | Binding | Inhibition of p97-C522A mutant (unknown origin) after 10 mins by malachite green dye based visible spectrophotometry | Homo sapiens | 1 | ALA3797126 | assay format | Scientific Literature | |
14. | ALA3802593 | Binding | Reversible inhibition of p97 (unknown origin) ATPase activity at 100 uM after prolonged incubation of 6 hrs followed by dialysis for 2 hrs by malachite green dye based visible spectrophotometry | Homo sapiens | 1 | ALA3797126 | single protein format | Scientific Literature | |
15. | ALA3802594 | Binding | Non-competitive inhibition of p97 (unknown origin) ATPase activity at IC50 in presence of varoius concentration of ATP | Homo sapiens | 1 | ALA3797126 | single protein format | Scientific Literature | |
16. | ALA3802598 | Binding | Inhibition of p97 (unknown origin) ATPase in HEK293 cells expressing UbG76VGFP fusion protein assessed as level of UbG76VGFP rescued by measuring GFP fluorescence loss by flow cytometry | Homo sapiens | 2 | ALA3797126 | cell-based format | Scientific Literature | |
17. | ALA3802599 | Binding | Inhibition of p97 (unknown origin) ATPase in HEK293 cells expressing T cell receptor alpha-GFP assessed as level of T cell receptor alpha-GFP rescued by flow cytometry | Homo sapiens | 2 | ALA3797126 | cell-based format | Scientific Literature | |
18. | ALA3802600 | Binding | Reversible inhibition of p97 (unknown origin) ATPase activity after prolonged incubation followed by dialysis | Homo sapiens | 1 | ALA3797126 | single protein format | Scientific Literature | |
19. | ALA3802601 | Binding | Inhibition of p97 (unknown origin) ATPase activity up to 200 uM after 10 mins by malachite green dye based visible spectrophotometry | Homo sapiens | 2 | ALA3797126 | single protein format | Scientific Literature | |
20. | ALA1102721 | Binding | Inhibition of VCP assessed as conversion of ATP to ADP by enzyme coupled glucokinase assay | 27 | ALA1154941 | single protein format | Scientific Literature |