# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1107602 | B | Inhibition of Pseudomonas aeruginosa LasB by fluorimetric assay in buffer of pH 7.2 | Pseudomonas aeruginosa | 160 | ALA1154297 | single protein format | Scientific Literature | |
2. | ALA2150722 | F | Antagonist activity at Pseudomonas aeruginosa LasB using Abz-Ala-Gly-Leu-Ala-p-Nitro-Benzyl-Amide as substrate incubated for 30 mins prior to substrate addition by FRET assay | Pseudomonas aeruginosa | 13 | ALA2146446 | assay format | Scientific Literature | |
3. | ALA2150723 | F | Antagonist activity at Pseudomonas aeruginosa PA14 LasB assessed as inhibition of swarming at 25 uM after 16 hrs swarming motility assay relative to control | Pseudomonas aeruginosa PA14 | 4 | ALA2146446 | assay format | Scientific Literature | |
4. | ALA3376524 | B | Inhibition of Pseudomonas aeruginosa pseudolysin using Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)-OH as substrate after 1 hr by fluorescence assay in presence of Brij-35 | Pseudomonas aeruginosa | 14 | ALA3351501 | single protein format | Scientific Literature | |
5. | ALA3376525 | B | Inhibition of Pseudomonas aeruginosa pseudolysin using Abz-Ala-Gly-Leu-Ala-p-nitrobenzylamide as substrate after 15 mins | Pseudomonas aeruginosa | 14 | ALA3351501 | single protein format | Scientific Literature | |
6. | ALA3404730 | B | Antagonist activity at lasB in Pseudomonas aeruginosa harboring lasB-gfp gene assessed as inhibition of 3-oxo-C6-HSL-induced quorum sensing by green fluorescence protein reporter assay | Pseudomonas aeruginosa | 13 | ALA3400119 | assay format | Scientific Literature | |
7. | ALA3756035 | B | Inhibition of Bacillus thermoproteolyticus pseudolysin pretreated with compound for 15 mins followed by the addition of Abz-Ala-Gly-leu-Ala-p-nitrobenzalamide as substrate by spectrofluorometry | Pseudomonas aeruginosa | 10 | ALA3751806 | single protein format | Scientific Literature | |
8. | ALA3756036 | B | Inhibition of Pseudomonas aeruginosa pseudolysin pretreated with compound for 1 hr followed by the addition of 1.73 uM bradykinin like substrate by spectrofluorometry | Pseudomonas aeruginosa | 10 | ALA3751806 | single protein format | Scientific Literature | |
9. | ALA4146049 | B | Inhibition of Pseudomonas aeruginosa PAO1 GFP-fused quorum sensing lasB at 10 uM measured every 15 mins up to 12 hrs by GFP reporter gene assay relative to control | Pseudomonas aeruginosa PAO1 | 28 | ALA4145447 | single protein format | Scientific Literature | |
10. | ALA4268463 | B | Inhibition of Pseudomonas aeruginosa PAO1 GFP-fused quorum sensing lasB up to 40 uM measured every 15 mins up to 12 hrs by GFP reporter gene assay | Pseudomonas aeruginosa PAO1 | 1 | ALA4265949 | single protein format | Scientific Literature | |
11. | ALA4268468 | B | Inhibition of Pseudomonas aeruginosa PAO1 GFP-fused quorum sensing lasB assessed as decrease in elastase activity up to 40 uM incubated for 24 hrs recorded every 10 mins for 3 hrs | Pseudomonas aeruginosa PAO1 | 1 | ALA4265949 | single protein format | Scientific Literature | |
12. | ALA4383805 | B | Inhibition of lasB quorum sensing system in Pseudomonas aeruginosa PAO1 harboring mini-ctx-lasB-lacZ after 24 hrs by beta-galactosidase reporter gene assay | Pseudomonas aeruginosa PAO1 | 1 | ALA4382219 | single protein format | Scientific Literature | |
13. | ALA4383808 | B | Inhibition of lasB quorum sensing system in Pseudomonas aeruginosa PAO1 assessed as reduction in elastase release at 10 uM after 24 hrs relative to control | Pseudomonas aeruginosa PAO1 | 1 | ALA4382219 | single protein format | Scientific Literature | |
14. | ALA4383810 | B | Inhibition of lasB quorum sensing system in Pseudomonas aeruginosa PAO1 harboring mini-ctx-lasB-lacZ at 50 uM in presence of ODdHL by beta-galactosidase reporter gene assay | Pseudomonas aeruginosa PAO1 | 1 | ALA4382219 | single protein format | Scientific Literature | |
15. | ALA4423833 | B | Inhibition of lasB in Pseudomonas aeruginosa PAO1 containing lasB-lacZ translational reporter fusion at 20 uM after 16 hrs by LacZ reporter gene assay | Pseudomonas aeruginosa PAO1 | 2 | ALA4422639 | single protein format | Scientific Literature | |
16. | ALA4621227 | B | Inhibition of Pseudomonas aeruginosa PA14 LasB using 2-Aminobenzoyl-AlaGly-Leu-Ala-4-Nitrobenzylamide as fluorogenic substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by FRET assay | Pseudomonas aeruginosa PA14 | 32 | ALA4619769 | single protein format | Scientific Literature | |
17. | ALA4621250 | B | Inhibition of Pseudomonas aeruginosa LasB using aminobenzoyl-Ala-Gly-Leu-Ala-p-nitro-benzyl-amide as fluorogenic substrate by fluorimetric assay | Pseudomonas aeruginosa | 1 | ALA4619769 | single protein format | Scientific Literature | |
18. | ALA4670240 | B | Inhibition of lasB fluorescence in Pseudomonas aeruginosa PAO1 transfected with GFP by measuring fluorescence intensity every 15 mins for 12 hrs by microtiter plate reader assay | Pseudomonas aeruginosa PAO1 | 1 | ALA4665779 | assay format | Scientific Literature | |
19. | ALA5108267 | B | Inhibition of Pseudomonas aeruginosa PA14 LasB elastase preincubated for 15 mins followed by substrate addition and measured after 60 mins using 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide as fluorogenic substrate by fluorescence based microplate reader analysis | Pseudomonas aeruginosa | 21 | ALA5107943 | single protein format | Scientific Literature | |
20. | ALA5112267 | A | Prodrug activation assessed as LasB- mediated drug release in Pseudomonas aeruginosa PA14 cell supernatant and measured after 24 hrs by HPLC-MS analysis | Pseudomonas aeruginosa | 2 | ALA5108061 | single protein format | Scientific Literature |