Inhibition of Influenza A virus A/PR/8/34 HA1 domain assessed as reduction in virus-induced aggregation of chicken erythrocytes at 0.75 to 1500 uM incubated for 1 hr with virus followed by transfer of compound-virus preparation to chicken erythrocytes solution measured after 40 mins
Inhibition of Influenza A virus A/PR/8/34 HA2-mediated hemolysis preincubated with virus for 30 mins followed by chicken red blood cells addition measured after 30 mins by spectrophotometric analysis
Inhibition of Influenza A virus (A/PR/8/34(H1N1)) HA-mediated chicken erythrocytes hemagglutination preincubated for 30 mins followed by erythrocytes addition and measured for 1 hr
Inhibition of Hemagglutinin in Influenza A virus (A/Puerto Rico/8/1934(H1N1)) assessed as reduction in virus-induced aggregation of chicken erythrocytes at 3.125 to 50 ug/mL incubated for 60 mins by hemagglutination assay
Inhibition of Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Hemagglutinin expressed in African green monkey Vero cells assessed as reduction in virus-induced syncytium formation at 50 ug/ml pre-treated with trypsin followed by compound addition for 15 mins and measured after 2 hrs at pH 5 by HA syncytium assay
Inhibition of conformational changes in Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Hemagglutinin assessed as increase in retention amount of HA1 subunit on HA proteins at 25 to 50 ug/ml added before acidification incubated for 1 hr by conformational change inhibition assay
Inhibition of trypsin-mediated cleavage of Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Hemagglutinin (HA0) at 50 ug/ml incubated for 30 mins by Coomassie blue fast staining and SDS-PAGE gel based trypsin susceptibility assay
Inhibition of Hemagglutinin in Influenza A virus (A/Puerto Rico/8/1934(H1N1))/H1N1 pseudovirus infected in 293FT cells assessed as reduction in virus infection at 50 ug/ml pre-treated with virus before infection and measured after 48 hrs by inverted fluorescence microscopy
Inhibition of Hemagglutinin in Influenza A virus (A/Puerto Rico/8/34(H1N1)) assessed as reduction in virus-induced agglutination of chicken erythrocytes at 50 uM
Inhibition of Hemagglutinin in Influenza A virus (A/Puerto Rico/8/34(H1N1)) assessed as reduction in virus-induced agglutination of chicken erythrocytes at 100 uM
Inhibition of Influenza A virus (A/PR 8/34 (H1N1)) hemagglutinin transfected in human HeLa cells assessed as reduction in low pH-indued polykaryon formation at 100 uM preincubated for 15 mins followed by pH 5.2 buffer addition and measured after 5 mins by giemsa staining based microscopic analysis
Inhibition of Influenza A virus (A/PR 8/34 (H1N1)) hemagglutinin transfected in human HeLa cells assessed as reduction in low pH-indued polykaryon formation preincubated for 15 mins followed by pH 5.2 buffer addition and measured after 5 mins by giemsa staining based microscopic analysis