# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3801640 | Binding | Inhibition of human poly His-tagged WDR5 (1 to 334 residues) interaction with MLL1 assessed as displacement of fluorescein isothiocyanate labelled H3 (1 to 15 residues) peptide from WDR5 expressed in Escherichia coli BL21(DE3)-V2R-pRARE2 cells by fluorescence polarization assay | Homo sapiens | 54 | ALA3797181 | cell-based format | Scientific Literature | |
2. | ALA3801641 | Binding | Inhibition of human poly His-tagged WDR5 (1 to 334 residues) interaction with MLL1 assessed as displacement of fluorescein isothiocyanate labelled 9-Ala-FAM peptide substrate from WDR5 expressed in Escherichia coli BL21(DE3)-V2R-pRARE2 cells by fluorescence polarization assay | Homo sapiens | 9 | ALA3797181 | cell-based format | Scientific Literature | |
3. | ALA3801642 | Binding | Binding affinity to human poly His-tagged WDR5 (1 to 334 residues) expressed in Escherichia coli BL21(DE3)-V2R-pRARE2 cells by surface plasmon resonance analysis | Homo sapiens | 14 | ALA3797181 | cell-based format | Scientific Literature | |
4. | ALA3801643 | Binding | Inhibition of N-terminal His-tagged WDR5 (24 to 334 residues) (unknown origin) interaction with MLL1 assessed as displacement of 5-Lys-FAM peptide substrate from WDR5 expressed in Escherichia coli Rosetta2(DE3) pLysS cells by fluorescence polarization assay | Homo sapiens | 1 | ALA3797181 | cell-based format | Scientific Literature | |
5. | ALA3801645 | Binding | Inhibition of SUMO-His-tagged WDR5 (unknown origin) interaction with MLL1 assessed as displacement of fluorescence labelled Ac-ARA peptide substrate from WDR5 expressed in Escherichia coli BL21(DE3) pLysS cells by fluorescence polarization assay | Homo sapiens | 1 | ALA3797181 | cell-based format | Scientific Literature | |
6. | ALA3803350 | Binding | Inhibition of WDR5 interaction with MLL1 in human AML cells expressing mutant N-terminal C/EBPalpha assessed as reduction in cell viability at 5 uM relative to control | Homo sapiens | 1 | ALA3797181 | cell-based format | Scientific Literature | |
7. | ALA3824741 | Binding | Binding affinity to His-tagged human WDR5 expressed in Escherichia coli by isothermal titration calorimetry | Homo sapiens | 2 | ALA3822451 | assay format | Scientific Literature | |
8. | ALA3824742 | Binding | Inhibition of 10mer-Thr-FAM probe binding to His-tagged human WDR5 expressed in Escherichia coli incubated for 2 hrs by fluorescence polarization assay | Homo sapiens | 25 | ALA3822451 | assay format | Scientific Literature | |
9. | ALA3824743 | Binding | Binding affinity to His-tagged human WDR5 expressed in Escherichia coli BL21 by isothermal titration calorimetry | Homo sapiens | 1 | ALA3822451 | assay format | Scientific Literature | |
10. | ALA1118768 | Binding | Binding affinity to WDR5 expressed in pLysS cells by optimized fluorescent polarization based competitive binding assay | 25 | ALA1155441 | cell-based format | Scientific Literature | ||
11. | ALA1118769 | Binding | Binding affinity to WDR5 expressed in pLysS cells after 24 hrs by optimized fluorescent polarization based saturation binding assay | 5 | ALA1155441 | cell-based format | Scientific Literature | ||
12. | ALA2339612 | Binding | Displacement of MLL-WIN N-terminal FITC-tagged GSARAEVHLRKS peptide from human WDR5 (1-334) by fluorescence polarization assay | Homo sapiens | 1 | ALA2331202 | single protein format | Scientific Literature | |
13. | ALA2339613 | Binding | Displacement of N-terminal isothiocyanate-fluorescein-labeled H3(ARTKQTARKSTGGKA) peptide from human WDR5 expressed in Escherichia coli BL21(DE3) by fluorescence polarization assay | Homo sapiens | 39 | ALA2331202 | assay format | Scientific Literature | |
14. | ALA2339614 | Binding | Antagonist activity at WDR5 (unknown origin) | Homo sapiens | 1 | ALA2331202 | single protein format | Scientific Literature | |
15. | ALA3859082 | Binding | Binding affinity to WDR5 (unknown origin) at 200 uM by DSF assay | Homo sapiens | 1 | ALA3856251 | single protein format | Scientific Literature | |
16. | ALA3859786 | Binding | Inhibition of C-terminal 5-FAM-ARTEVHLRKS binding to WDR5 (unknown origin) by fluorescence polarization assay | Homo sapiens | 22 | ALA3856283 | single protein format | Scientific Literature | |
17. | ALA3859794 | Binding | Inhibition of C-terminal 5-FAM-WIN peptide binding to N-terminal His-tagged WDR5 (24 to 334 residues) (unknown origin) expressed in Rosetta2-(DE3) pLysS cells after 5 hrs by fluorescence polarization assay | Homo sapiens | 1 | ALA3856283 | cell-based format | Scientific Literature | |
18. | ALA3859796 | Binding | Inhibition of N-terminal FITC-GSARAEVHLRKS binding to WDR5 (unknown origin) by fluorescence polarization assay | Homo sapiens | 1 | ALA3856283 | single protein format | Scientific Literature | |
19. | ALA3875502 | Binding | Inhibition of 10mer-Thr-FAM probe binding to human WDR5 after 2 hrs by fluorescence polarization assay | Homo sapiens | 48 | ALA3875159 | single protein format | Scientific Literature | |
20. | ALA3875503 | Binding | Binding affinity to human WDR5 by isothermal titration calorimetry | Homo sapiens | 3 | ALA3875159 | single protein format | Scientific Literature |