Induction of cell cycle arrest in human HCC1806 cells assessed as decrease in G2/M phase cells at 10 uM incubated for 24 hrs by flow cytometric analysis
Antitumor activity against human HCC1806 cells assessed as induction of phosphorylated p38 protein accumulation at 10 uM incubated for 24 hrs by Western blot analysis
Antitumor activity against human HCC1806 cells assessed as induction of p21 protein accumulation at 10 uM incubated for 24 hrs by Western blot analysis
Cytotoxicity against ER, PR, HER2-deficient human HCC1806 cells as reduction of survival factor at IC50 incubated for 48 hrs measured after 12 days by crystal violet staining-based clonogenic assay
Cytotoxicity against ER, PR, HER2-deficient human HCC1806 cells as reduction of total protein content at IC50 incubated for 48 hrs by SRB assay relative to control
Induction of apoptosis in triple negative ER, PR, HER2-deficient human HCC1806 cells assessed as increase of late apoptotic/nectrotic cells at IC50 after 48 hrs by Annexin V/propidium iodide staining-based flow cytometry relative to control
Induction of apoptosis in triple negative ER, PR, HER2-deficient human HCC1806 cells assessed as decrease of viable cells at IC50 after 48 hrs by Annexin V/propidium iodide staining-based flow cytometry relative to control
Induction of apoptosis in triple negative ER, PR, HER2-deficient human HCC1806 cells assessed as increase of necrotic cells at IC50 after 48 hrs by Annexin V/propidium iodide staining-based flow cytometry relative to control
Induction of apoptosis in ER, PR, HER2-deficient human HCC1806 cells as increase in Bax/Bcl2 expression ratio at IC50 after 48 hrs by Western blotting analysis relative to control