# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1832850 | B | Binding affinity to second bromodomain of BRD4 assessed as change in melting temperature at 100 uM by differential scanning fluorimetry | 9 | single protein format | Scientific Literature | |||
2. | ALA1832851 | B | Binding affinity to first bromodomain of BRD4 assessed as change in melting temperature at 100 uM by differential scanning fluorimetry | 9 | single protein format | Scientific Literature | |||
3. | ALA1832996 | B | Displacement of H4Ac4 peptide from first bromodomain of human BRD4 by peptide displacement assay | Homo sapiens | 9 | single protein format | Scientific Literature | ||
4. | ALA1833001 | B | Inhibition of first bromodomain of BRD4 | 1 | single protein format | Scientific Literature | |||
5. | ALA1953102 | B | Binding affinity to His-6 tagged BRD4 expressed in Escherichia coli at 100 uM after 60 mins by fluorescence anisotropic analysis | 4 | assay format | Scientific Literature | |||
6. | ALA1953105 | B | Inhibition of His-6 tagged BRD4-RD12 precoupled with biotinylated tetra-acetylated histone H4 expressed in Escherichia coli assessed as protein-protein interaction at 50 uM after 1 hr by TR-FRET assay | 4 | assay format | Scientific Literature | |||
7. | ALA1953108 | B | Inhibition of His-6 tagged BRD4 expressed in Escherichia coli after 60 mins by fluorescence anisotropic analysis | 2 | assay format | Scientific Literature | |||
8. | ALA1953111 | B | Inhibition of His-6 tagged BRD4-RD12 interaction precoupled with biotinylated tetra-acetylated histone H4 expressed in Escherichia coli after 1 hr by TR-FRET assay | 1 | assay format | Scientific Literature | |||
9. | ALA1958930 | B | Binding affinity to BRD4-BD1 assessed as change in melting temperature at 100 uM by protein stability shift assay | 12 | single protein format | Scientific Literature | |||
10. | ALA1958939 | B | Binding affinity to BRD4-BD1 assessed as change in melting temperature at 10 uM by protein stability shift assay | 6 | single protein format | Scientific Literature | |||
11. | ALA1959064 | B | Binding affinity to BRD4-BD1 assessed as change in melting temperature by isothermal titration calorimetry | 3 | single protein format | Scientific Literature | |||
12. | ALA1959065 | B | Binding affinity to BRD4-BD1 by isothermal titration calorimetry | 3 | single protein format | Scientific Literature | |||
13. | ALA1959068 | B | Binding affinity to BRD4-BD2 assessed as change in melting temperature at 100 uM by protein stability shift assay | 12 | single protein format | Scientific Literature | |||
14. | ALA1959071 | B | Binding affinity to BRD4-BD2 assessed as change in melting temperature at 10 uM by protein stability shift assay | 6 | single protein format | Scientific Literature | |||
15. | ALA1959072 | B | Binding affinity to BRD4 by isothermal titration calorimetry | 3 | single protein format | Scientific Literature | |||
16. | ALA1958561 | B | Binding affinity to BRD4-BD1 by isothermal titration calorimetry | 1 | single protein format | Scientific Literature | |||
17. | ALA1958562 | B | Binding affinity to BRD4-BD2 by isothermal titration calorimetry | 1 | single protein format | Scientific Literature | |||
18. | ALA1958566 | B | Binding affinity to BRD4-BD1,2 by isothermal titration calorimetry | 2 | single protein format | Scientific Literature | |||
19. | ALA1958568 | B | Displacement of acetylated histone peptide from BRD4-BD1 by luminescence proximity homogenous assay | 1 | single protein format | Scientific Literature | |||
20. | ALA1958569 | B | Displacement of acetylated histone peptide from BRD4-BD2 by luminescence proximity homogenous assay | 1 | single protein format | Scientific Literature |