Antagonist activity at human NOX4 expressed in CHO cell membrane coexpressing tetracylin repressor assessed as inhibition of ROS production after 20 mins by cell-free amplex red assay
Inhibition of full length human NOX4 transfected in COS 22 cells assessed as inhibition of Phorbol 12-myristate 13-acetate-stimulated ROS generation by measuring H2O2 production by fluorescence assay in presence of superoxide dismutase
Inhibition of NOX4 in human ARPE19 cells assessed as protection against 4-HNE-induced cell death by measuring cell viability recovery at 10 uM incubated for 24 hrs by MTT assay
Inhibition of NOX4 in human ARPE19 cells assessed as suppression of 4-HNE-induced superoxide production at 10 uM pre-incubated for 1 hr before 4-HNE addition for 1 hr by lucigenin chemiluminescence assay
Inhibition of NOX4 in human ARPE19 cells assessed as protection against 4-HNE-induced cell death by measuring cell viability recovery incubated for 24 hrs by MTT assay
Inhibition of Nox4 in HASMCs assessed as reduction in TGFbeta-induced increase in smooth muscle alpha actin level pretreated for 30 mins followed by TGFbeta stimulation after 24 hrs by Western blot analysis
Inhibition of human NOX4 assessed as reduction in ROS production using lucigenin as substrate in presence of NADPH by chemiluminescence based microplate reader analysis