Activity of Rce1p in yeast membranes assessed as enzyme-mediated Ca1a2X proteolysis measured every 30 to 60 seconds for 1 hr by spectrofluorimetric assay
Ratio of Vmax to Km for Rce1p in yeast membranes assessed as enzyme-mediated Ca1a2X proteolysis measured every 30 to 60 seconds for 1 hr by spectrofluorimetric assay
Activity of Rce1p in yeast membranes assessed as enzyme-mediated Ca1a2X proteolysis per mg of protein measured every 30 to 60 seconds for 1 hr by spectrofluorimetric assay
Binding affinity to Rce1p in yeast membranes assessed as reduction in binding affinity at 0.75 uM after 30 mins by Western blotting in presence of 50 uM of (3S,6S,9S,12S,15S,18R,21S,24S,27S)-3,9,15-tris(4-aminobutyl)-24-sec-butyl-21-(4-(3,5-dinitrophenylamino)butyl)-12-((S)-1-hydroxyethyl)-6-(hydroxymethyl)-22-imino-27-(2-(methylthio)ethyl)-1,4,7,10,13,16,19,25-octaoxo-1-phenyl-18-(((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienylthio)methyl)-2,5,8,11,14,17,20,23,26-nonaazaoctacosan-28-oic acid
Binding affinity to Rce1p in yeast membranes assessed as reduction in binding affinity at 0.75 uM after 30 mins by Western blotting in presence of 100 uM of (3S,6S,9S,12S,15S,18R,21S,24S,27S)-3,9,15-tris(4-aminobutyl)-24-sec-butyl-21-(4-(3,5-dinitrophenylamino)butyl)-12-((S)-1-hydroxyethyl)-6-(hydroxymethyl)-22-imino-27-(2-(methylthio)ethyl)-1,4,7,10,13,16,19,25-octaoxo-1-phenyl-18-(((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienylthio)methyl)-2,5,8,11,14,17,20,23,26-nonaazaoctacosan-28-oic acid