| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA677570 | B | Evaluated for inhibition of enoyl acyl carrier protein reductase (FabI) in Staphylococcus aureus. | Staphylococcus aureus | 21 | ALA1133895 | single protein format | Scientific Literature | |
| 2. | ALA677571 | B | Evaluated for inhibition of enoyl acyl carrier protein reductase (FabI) of Staphylococcus aureus in the presence of NADH using crotonoyl CoA as substrate | Staphylococcus aureus | 1 | ALA1133895 | assay format | Scientific Literature | |
| 3. | ALA677574 | B | Antibacterial activity against Staphylococcus aureus FabI | Staphylococcus aureus | 27 | ALA1133924 | single protein format | Scientific Literature | |
| 4. | ALA677578 | B | Inhibitory activity against Enoyl-ACP reductase FabI in Staphylococcus aureus | Staphylococcus aureus | 11 | ALA1136775 | single protein format | Scientific Literature | |
| 5. | ALA819485 | B | Concentration required for the 50% inhibition of enoyl-ACP reductase from Staphylococcus aureus was determined. | 14 | ALA1135515 | single protein format | Scientific Literature | ||
| 6. | ALA1039114 | B | Inhibition of Staphylococcus aureus FabI | Staphylococcus aureus | 11 | ALA1155988 | single protein format | Scientific Literature | |
| 7. | ALA1686899 | B | Inhibition of Staphylococcus aureus enoyl-ACP reductase assessed as increase of NADPH level | Staphylococcus aureus | 2 | ALA1681723 | single protein format | Scientific Literature | |
| 8. | ALA2188140 | B | Inhibition of Staphylococcus aureus recombinant FabI using trans-2-octenoyl N-acetylcysteamine thioester as substrate preincubated for 1 to 2 hrs | Staphylococcus aureus | 1 | ALA2176870 | single protein format | Scientific Literature | |
| 9. | ALA2188141 | B | Inhibition of Staphylococcus aureus recombinant FabI using trans-2-octenoyl N-acetylcysteamine thioester as substrate preincubated for 60 mins in presence of NADP+ | Staphylococcus aureus | 1 | ALA2176870 | single protein format | Scientific Literature | |
| 10. | ALA2188143 | B | Inhibition of Staphylococcus aureus recombinant FabI using trans-2-octenoyl N-acetylcysteamine thioester as substrate preincubated for 60 mins | Staphylococcus aureus | 14 | ALA2176870 | single protein format | Scientific Literature | |
| 11. | ALA2188150 | B | Inhibition of Staphylococcus aureus recombinant FabI using trans-2-octenoyl N-acetylcysteamine thioester as substrate preincubated for 60 mins in presence of NADPH | Staphylococcus aureus | 1 | ALA2176870 | single protein format | Scientific Literature | |
| 12. | ALA3385666 | B | Inhibition of Staphylococcus aureus enoyl ACP reductase | Staphylococcus aureus | 1 | ALA3352599 | single protein format | Scientific Literature | |
| 13. | ALA3365683 | B | Inhibition of Staphylococcus aureus FabI-mediated reduction of enoyl-ACP preincubated at 1 uM for 30 mins measured after 2 hrs by spectrophotometry | Staphylococcus aureus | 29 | ALA3352330 | single protein format | Scientific Literature | |
| 14. | ALA3365684 | B | Inhibition of Staphylococcus aureus FabI-mediated reduction of enoyl-ACP preincubated for 30 mins measured after 2 hrs by spectrophotometry | Staphylococcus aureus | 29 | ALA3352330 | single protein format | Scientific Literature | |
| 15. | ALA3399508 | B | Inhibition of Staphylococcus aureus FabI-mediated trans-2-octenoyl N-acetylcysteamine (t-o-NAC thioester) substrate reduction assessed as decrease in NADPH by ELISA | Staphylococcus aureus | 1 | ALA3397033 | single protein format | Scientific Literature | |
| 16. | ALA3625533 | B | Inhibition of Staphylococcus aureus FabI assessed as reduction in inhibition of reduction of trans-2-octenoyl N-acetylcysteamine substrate by spectrophotometry | Staphylococcus aureus | 54 | ALA3621046 | single protein format | Scientific Literature | |
| 17. | ALA3705967 | B | Inhibition Assay: The assay buffer AB contained 50 mM ADA (N-(2-acetamido)iminodiacetic acid monosodium salt) pH 6.5, 1 mM dithiothreitol, 0.006% Triton-X100 and 50 mM NaCl. The following components are added in a white polystyrene Costar plate (Ref 3912) up to a final volume of 55.5 uL: 1.5 uL DMSO or inhibitor dissolved in DMSO and 54 uL of a FabI/NADPH/NADP+ mixture in AB. After 60 min of pre-incubation at room temperature, the reaction is started by addition of 5 uL of trans-2-octenoyl N-acetylcysteamine thioester (t-o-NAC) to a final volume of 60.5 uL. This reaction mixture is then composed of 2 nM FabI, 40 uM NADPH (Sigma, N7505), 10 uM NADP+ (Sigma, N5755), 100 uM t-O-NAC and compound at defined concentration. Fluorescence intensity of NADPH (lamda=ex=360 nm, lamda=em=520 nm) is measured immediately after t-O-NAC addition (T0), and approximately 50 min later (T50) by a Fluostar Optima (BMG). | Staphylococcus aureus | 3 | ALA3638853 | single protein format | BindingDB Database | |
| 18. | ALA3705387 | B | Fluorescence Based Assay: The assay buffer "AB" contained 50 mM ADA (N-(2-acetamido)iminodiacetic acid monosodium salt) pH 6.5, 1 mM dithiothreitol, 0.006% Triton-X100 and 50 mM NaCl. The following components are added in a white polystyrene Costar plate (Ref 3912) up to a final volume of 55.5 uL: 1.5 uL DMSO or inhibitor dissolved in DMSO and 54 uL of a FabI/NADPH/NADP+ mixture in AB. After 60 min of pre-incubation at room temperature, the reaction is started by addition of 5 uL of trans-2-octenoyl N-acetylcysteamine thioester (t-o-NAC) to a final volume of 60.5 uL. This reaction mixture is then composed of 2 nM FabI, 40 uM NADPH (Sigma, N7505), 10 uM NADP+(Sigma, N5755), 100 uM t-O-NAC and compound at defined concentration. Fluorescence intensity of NADPH (lamda=ex=360 nm, lamda=em=520 nm) is measured immediately after t-O-NAC addition (T0), and approximately 50 min later (T50) by a Fluostar Optima (BMG) so as to achieve ~30% of NADPH conversion. | Staphylococcus aureus | 27 | ALA3638908 | single protein format | BindingDB Database | |
| 19. | ALA3705478 | B | Fluorescence Based Assay: Compound inhibitory activity of FabI enzyme is measured in vitro by the IC5 so determination using a fluorescence based assay.The protein FabI from S. aureus is prepared and purified using standard methods for recombinant protein expression after cloning of the gene in a prokaryotic expression vector.The biochemical activity of the FabI enzyme is assessed using the following method.The assay buffer AB contained 50 mM ADA (N-(2-acetamido)iminodiacetic acid monosodium salt) pH 6.5, 1 mM dithiothreitol, 0.006% Triton-X100 and 50 mM NaCl.The following components are added in a white polystyrene Costar plate (Ref 3912) up to a final volume of 55.5 uL: 1.5 uL DMSO or inhibitor dissolved in DMSO and 54 uL of a FabI/NADPH/NADP+ mixture in AB. After 60 min of pre-incubation at room temperature, the reaction is started by addition of 5 uL of trans-2-octenoyl N-acetylcysteamine thioester (t-o-NAC) to a final volume of 60.5 uL. | Staphylococcus aureus | 28 | ALA3638382 | assay format | BindingDB Database | |
| 20. | ALA4182166 | B | Inhibition of Staphylococcus aureus subsp. aureus Rosenbach ATCC 43300 FabI using crotonyl-CoA as substrate in presence of NADPH/NADH after 10 mins by fluorescence assay | Staphylococcus aureus subsp. aureus | 29 | ALA4177701 | single protein format | Scientific Literature |
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