| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1273387 | B | Competitive inhibition of rat His6x-tagged PRMT1 expressed in Escherichia coli BL21 (DE3) using H4(1-20) substrate by double-reciprocal plotting | Rattus norvegicus | 2 | assay format | Scientific Literature | ||
| 2. | ALA1273388 | B | Non-competitive inhibition of rat His6x-tagged PRMT1 expressed in Escherichia coli BL21 (DE3) using [14C]-AdoMet substrate by double-reciprocal plotting | Rattus norvegicus | 2 | assay format | Scientific Literature | ||
| 3. | ALA3129165 | B | Inhibition of rat recombinant PRMT1 using histone as substrate preincubated for 5 mins followed by substrate addition measured after 6.5 mins by liquid scintillation counting analysis in presence of [methyl-3H]-AdoMet | Rattus norvegicus | 6 | single protein format | Scientific Literature | ||
| 4. | ALA4026494 | B | Inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) using biotinylated H4 peptide (1 to 21 residues) as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins in presence of [3H]SAM by microbeta counting method | Rattus norvegicus | 18 | assay format | Scientific Literature | ||
| 5. | ALA4026495 | B | Inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) at 100 uM using biotinylated H4 peptide (1 to 21 residues) as substrate by TR-FRET assay | Rattus norvegicus | 7 | assay format | Scientific Literature | ||
| 6. | ALA4026496 | B | Uncompetitive inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) using biotinylated H4 peptide (1 to 21 residues) as substrate in presence of varying concentrations of [3H]SAM | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 7. | ALA4026497 | B | Inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) incubated for 15 mins followed by substrate addition measured after 60 mins by AlphaLisa method | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 8. | ALA4026498 | B | Binding affinity to rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) at 200 uM by 1D 1H CPMG-NMR spectroscopic method | Rattus norvegicus | 3 | assay format | Scientific Literature | ||
| 9. | ALA4026499 | B | Binding affinity to rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) at 200 uM by STD-NMR spectroscopic method | Rattus norvegicus | 3 | assay format | Scientific Literature | ||
| 10. | ALA4026500 | B | Non-competitive inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) in presence of varying concentrations of biotinylated H4 peptide (1 to 21 residues) substrate and fixed [3H]SAM concentration | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 11. | ALA4324378 | B | Inhibition of recombinant rat His-tagged PRMT1 expressed in Escherichia coli BL21(DE3) cells assessed as enzyme activity at 3.7 uM using H4-21 peptide as susbtrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4324386 | B | Inhibition of recombinant rat His-tagged PRMT1 expressed in Escherichia coli BL21(DE3) cells assessed as enzyme activity at 11 uM using H4-21 peptide as susbtrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4324394 | B | Inhibition of recombinant rat His-tagged PRMT1 expressed in Escherichia coli BL21(DE3) cells assessed as enzyme activity at 33 uM using H4-21 peptide as susbtrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4324402 | B | Inhibition of recombinant rat His-tagged PRMT1 expressed in Escherichia coli BL21(DE3) cells assessed as enzyme activity at 100 uM using H4-21 peptide as susbtrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4324410 | B | Inhibition of recombinant rat His-tagged PRMT1 expressed in Escherichia coli BL21(DE3) cells using H4-21 peptide as susbtrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4392212 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 3.3 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 17. | ALA4392218 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 10 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 18. | ALA4392224 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 33 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 19. | ALA4392230 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 100 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 20. | ALA4392236 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay | Rattus norvegicus | 1 | assay format | Scientific Literature |
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