Antagonist activity at human NOX2 in human polymorphonuclear cell membrane assessed as inhibition of ROS production after 20 mins by cell-free amplex red assay
Inhibition of NOX2 in human THP1 cells assessed as suppression of TPA-induced membrane translocation of p47phox at 20 uM incubated for 1 hr prior to TPA challenge measured after 12 hrs by Western blot analysis
Inhibition of NOX2 in human THP1 cells assessed as suppression of TPA-induced intracellular ROS accumulation at 20 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by flow cytometric analysis
Inhibition of NOX2 in human THP1 cells assessed as suppression of TPA-induced cell adhesion at 20 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by microscopic analysis
Inhibition of NOX2 in human THP1 cells assessed as downregulation of TPA-induced CD36 mRNA expression at 0.2 to 2 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis
Inhibition of NOX2 in human THP1 cells assessed as downregulation of TPA-induced CD14 mRNA expression at 0.2 to 2 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis
Inhibition of NOX2 in human THP1 cells assessed as downregulation of TPA-induced CD11b mRNA expression at 0.2 to 2 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis
Inhibition of NOX2 in human THP1 cells assessed as downregulation of TPA-induced CD36 mRNA expression at 20 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis
Inhibition of NOX2 in human THP1 cells assessed as downregulation of TPA-induced CD14 mRNA expression at 20 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis
Inhibition of NOX2 in human THP1 cells assessed as downregulation of TPA-induced CD11b mRNA expression at 20 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis
Inhibition of full length human NOX2 transfected in COS 22 cells assessed as inhibition of lithium dodecyl sulphate-stimulated ROS generation at 6.3 uM preincubated for 15 mins followed by lithium dodecyl sulphate challenge by cell-free assay in presence of NADPH
Inhibition of full length human NOX2 transfected in COS 22 cells assessed as inhibition of Phorbol 12-myristate 13-acetate-stimulated ROS generation by chemiluminescence assay
Inhibition of full length human NOX2 transfected in COS 22 cells assessed as inhibition of Phorbol 12-myristate 13-acetate-stimulated ROS generation by measuring H2O2 production by fluorescence assay in presence of superoxide dismutase
Inhibition of full length human NOX2 transfected in COS 22 cells assessed as inhibition of lithium dodecyl sulphate-stimulated ROS generation preincubated for 15 mins followed by lithium dodecyl sulphate challenge by cell-free assay in presence of NADPH
Inhibition of human Nox2 expressed in COS7 cells co-transfected with human p22-phox,p47phox and p67-phox assessed as inhibition of LiDS-induced superoxide generation at 6.3 uM preincubated for 15 mins followed by addition of LiDS and NADPH
Inhibition of human Nox2 expressed in COS7 cells co-transfected with human p22-phox,p47phox and p67-phox assessed as inhibition of PMA-induced superoxide generation by L-012 chemiluminescence analysis
Inhibition of human Nox2 expressed in COS7 cells co-transfected with human p22-phox,p47phox and p67-phox assessed as inhibition of PMA-induced H2O2 production by amplex red dye based fluorescence assay