| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1069650 | B | Inhibition of human 15PGDH expressed in Escherichia coli BL-21 by fluorescence spectrophotometry | Homo sapiens | 31 | ALA1156934 | assay format | Scientific Literature | |
| 2. | ALA1614125 | F | PUBCHEM_BIOASSAY: Confirmation qHTS Assay for Inhibitors of HPGD (15-Hydroxyprostaglandin Dehydrogenase). (Class of assay: confirmatory) | Homo sapiens | 79 | ALA1201862 | assay format | PubChem BioAssays | |
| 3. | ALA1614038 | F | PUBCHEM_BIOASSAY: qHTS Assay for Inhibitors of HPGD (15-Hydroxyprostaglandin Dehydrogenase). (Class of assay: confirmatory) [Related pubchem assays: 2429 (Confirmation qHTS Assay for Inhibitors of HPGD (15-Hydroxyprostaglandin Dehydrogenase)), 2407 (Probe Development Summary for Inhibitors of HPGD (15-Hydroxyprostaglandin Dehydrogenase)), 2427 (Thermal Shift Assay for Inhibitors of HPGD (15-Hydroxyprostaglandin Dehydrogenase))] | Homo sapiens | 23,945 | ALA1201862 | assay format | PubChem BioAssays | |
| 4. | ALA1803826 | B | Inhibition of human 15-PGDH in human A549 cells assessed as increase in IL1-beta stimulated PGE2 production after 5 hrs by enzyme immunoassay | Homo sapiens | 2 | ALA1799939 | cell-based format | Scientific Literature | |
| 5. | ALA1803827 | B | Inhibition of human 15-PGDH in human A549 cells assessed as increase in IL1-beta stimulated PGE2 production at 5 uM after 5 hrs by enzyme immunoassay | Homo sapiens | 4 | ALA1799939 | cell-based format | Scientific Literature | |
| 6. | ALA1803828 | B | Inhibition of human 15-PGDH expressed in Escherichia coli using PGE2 as substrate and NAD+ as coenzyme assessed as formation of NADH by spectrophotometric analysis | Homo sapiens | 9 | ALA1799939 | assay format | Scientific Literature | |
| 7. | ALA2415234 | B | Inhibition of 15-PGDH in IL-1beta-stimulated human A549 cells assessed as increase in PGE2 level at 5 uM after 5 hrs by enzyme immunoassay relative to control | Homo sapiens | 4 | ALA2413000 | cell-based format | Scientific Literature | |
| 8. | ALA2415235 | B | Inhibition of 15-PGDH in IL-1beta-stimulated human A549 cells assessed as increase in PGE2 level after 5 hrs by enzyme immunoassay | Homo sapiens | 4 | ALA2413000 | cell-based format | Scientific Literature | |
| 9. | ALA2415236 | B | Inhibition of human GST-fused 15-PGDH expressed in Escherichia coli BL21 using PGE2 as substrate assessed as formation of NADH by fluorescence spectrophotometric analysis | Homo sapiens | 35 | ALA2413000 | assay format | Scientific Literature | |
| 10. | ALA3107135 | B | Inhibition of PGDH (unknown origin) | Homo sapiens | 2 | ALA3102731 | single protein format | Scientific Literature | |
| 11. | ALA3107136 | B | Inhibition of human HPGD in human ME180 cells assessed as downregulation of TNFalpha-induced NF-kappaB expression at 647 pM to 38.2 uM incubated for 15 mins prior to TNFalpha addition measured after 4 to 24 hrs by beta-lactamase reporter gene assay | Homo sapiens | 7 | ALA3102731 | cell-based format | Scientific Literature | |
| 12. | ALA3107150 | B | Inhibition of human HPGD using PGE2 as substrate after 15 mins by fluorescence assay | Homo sapiens | 55 | ALA3102731 | single protein format | Scientific Literature | |
| 13. | ALA3254514 | A | Stability of the compound assessed as prostaglandin 15-hydroxydehydrogenase (unknown origin)-mediated metabolite formation | Homo sapiens | 22 | ALA3244135 | single protein format | Scientific Literature | |
| 14. | ALA3256736 | A | Metabolic stability of the compound assessed as 15-hydroxyprostanoid dehydrogenase (unknown origin)-mediated metabolite formation | Homo sapiens | 1 | ALA3243919 | single protein format | Scientific Literature | |
| 15. | ALA3404953 | B | Inhibition of 15-PGDH (unknown origin) by fluorescence spectrophotometry | Homo sapiens | 1 | ALA3399956 | single protein format | Scientific Literature | |
| 16. | ALA3404954 | B | Inhibition of 15-PGDH in human A549 cells assessed as increase in PGE2 level after 12 hrs by immunoassay | Homo sapiens | 1 | ALA3399956 | cell-based format | Scientific Literature | |
| 17. | ALA3404955 | B | Inhibition of 15-PGDH in human A549 cells assessed as increase in PGE2 level at 5 uM after 12 hrs by immunoassay relative to control | Homo sapiens | 2 | ALA3399956 | cell-based format | Scientific Literature | |
| 18. | ALA3562140 | F | PubChem BioAssay. Extended Characterization of HPGD Inhibitors: Activity in Primary Assay (HPGD). (Class of assay: confirmatory) | 82 | ALA1201862 | assay format | PubChem BioAssays | ||
| 19. | ALA3705358 | B | Inhibition Assay: Experimental was performed by measuring the formation of NADH at 340 nm with a fluorescence spectrophotometer. Specifically, 2 ml (in total) of the solution containing 50 mM Tris-HCl (pH 7.5), 0.1 mM DTT, 0.25 mM NAD+, 10 μg of purified 15-PGDH enzyme, 21 μM PGE2 and various concentrations (0.0001 μM to 64 μM) of the derivatives according to the present invention was added to each cell. The absorbance of the reaction mixture was recorded at 340 nm so that the activities of the derivatives according to the present invention as 15-PGDH inhibitors were determined from a standard curve prepared from the absorbance of various concentrations of NADH at 340 nm. | Homo sapiens | 124 | ALA3639291 | single protein format | BindingDB Database | |
| 20. | ALA4051488 | B | Inhibition of recombinant human C-terminal 6xHis-tagged 15-PGDH expressed in Escherichia coli using PGE2 as substrate after 15 mins in presence of NAD(+) by fluorescence assay | Homo sapiens | 68 | ALA4049436 | assay format | Scientific Literature |
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