#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1614381	F	PUBCHEM_BIOASSAY: Confirmation Assay for Inhibitors of Human Galactosidase (GALK): probe SAR. (Class of assay: confirmatory) [Related pubchem assays: 1868, 2114, 1379 ]	Homo sapiens	27		assay format	PubChem BioAssays
2.	ALA1613821	F	PUBCHEM_BIOASSAY: Confirmation Assay for Inhibitors of Human Galactosidase (GALK). (Class of assay: confirmatory) [Related pubchem assays: 1868, 1379 ]	Homo sapiens	129		assay format	PubChem BioAssays
3.	ALA1738082	F	PUBCHEM_BIOASSAY: qHTS Validation Assay for Inhibitors of Human Galactosidase (GALK). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1868, AID2114]	Homo sapiens	9		assay format	PubChem BioAssays
4.	ALA1738237	F	PUBCHEM_BIOASSAY: Confirmation Assay for Inhibitors of Human Galactosidase (GALK): SAR round 2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1868, AID2015, AID2114, AID2499, AID2502, AID2506, AID2547]	Homo sapiens	19		assay format	PubChem BioAssays
5.	ALA1837840	B	Inhibition of human GALK1 up to 50 uM	Homo sapiens	9		single protein format	Scientific Literature
6.	ALA2020225	B	Inhibition of human galactosidase after 30 mins by Kinase-GloTM assay	Homo sapiens	13		single protein format	Scientific Literature
7.	ALA2020226	B	Inhibition of galactosidase in human primary fibroblasts derived from GALT-deficient patients assessed as reduction in accumulation of gal-1P at 50 uM preincubated overnight prior galactose challenge measured after 4 hrs	Homo sapiens	3		single protein format	Scientific Literature
8.	ALA2020227	B	Inhibition of galactosidase in human primary fibroblasts derived from GALT-deficient patients assessed as reduction in accumulation of gal-1P at 100 uM preincubated overnight prior galactose challenge measured after 4 hrs	Homo sapiens	1		single protein format	Scientific Literature
9.	ALA2020233	B	Inhibition of human galactosidase at >50 uM after 30 mins by Kinase-GloTM assay	Homo sapiens	6		single protein format	Scientific Literature
10.	ALA3887026	B	qHTS Assay: Assay details and protocol: The primary assay monitored ATP depletion using Promega's KinaseGlo technology, where ATP levels are measured through luminescence generated from firefly luciferase, a bioluminescent ATP-dependent enzymes. ATP was held at 35 μM, near its reported KM value, and the KM for galactose was determined under the 1536-well assay conditions to be 50-100M (FIG. 1A). As well, the IC50 for a commercially available CD45 inhibitor (N-(9,10-dioxo-9,10-dihydrophenanthren-2-yl)pivalamide) was confirmed (previously found to inhibit GALK (FIG. 1B)). This was used as the positive control for the assay. The assay used 5 nM GALK and a 1 hr incubation time, which gave sufficient signal:background and stability for the HTS.		88		single protein format	BindingDB Database
11.	ALA4477063	B	Inhibition of His6-tagged recombinant human GALK expressed in Escherichia coli HMS174 (DE3) incubated for 60 mins using galactose and ATP by Kinase-Glo reagent based assay	Homo sapiens	34		assay format	Patent Bioactivity Data
12.	ALA4477066	B	Inhibition of His6-tagged recombinant human GALK expressed in Escherichia coli HMS174 (DE3) by pyruvate kinase/lactate dehydrogenase-coupled assay	Homo sapiens	4		assay format	Patent Bioactivity Data
13.	ALA4477068	B	Inhibition of His6-tagged recombinant human GALK expressed in Escherichia coli HMS174 (DE3) at 5 to 15 uM incubated for 60 mins using galactose and ATP by Kinase-Glo reagent based assay	Homo sapiens	1		assay format	Patent Bioactivity Data
14.	ALA5051778	B	Inhibition of recombinant human GALK1 using ATP as substrate incubated for 1 hr and measured by Kinase-Glo assay	Homo sapiens	284		single protein format	Patent Bioactivity Data
15.	ALA5052897	B	Inhibition of GALK (unknown origin) incubated for 1 hr and measured by qHTS assay	Homo sapiens	91		single protein format	Patent Bioactivity Data
16.	ALA5052898	B	Inhibition of GALK (unknown origin) incubated for 1 hr and measured by qHTS assay realtive to control	Homo sapiens	91		single protein format	Patent Bioactivity Data
17.	ALA5098470	B	Inhibition of recombinant human GALK1 using galactose as substrate incubated for 1 hrs in the presence of ATP by Kinase-Glo reagent based luminescence assay	Homo sapiens	73		single protein format	Scientific Literature
18.	ALA5098476	B	Inhibition of human GALK1 in GALT-deficient human fibroblast assessed as reduction in gal-1P level incubated for 2 to 4 hrs by cellular Gal-1P accumulation assay	Homo sapiens	1		cell-based format	Scientific Literature
19.	ALA5098477	B	Inhibition of human GALK1 in GALT-deficient human fibroblast assessed as reduction in gal-1P level at 3 uM incubated for 2 to 4 hrs by cellular Gal-1P accumulation assay relative to control	Homo sapiens	1		cell-based format	Scientific Literature