#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2148625	B	Inhibition of human ROMK1 channel expressed in CHO cells coexpressing DHFR assessed as inhibition of 86Rb+ efflux after 35 mins by TopCount method	Homo sapiens	36		cell-based format	Scientific Literature
2.	ALA2148630	B	Inhibition of ROMK by electrophysiology assay		3		single protein format	Scientific Literature
3.	ALA2148638	B	Inhibition of ROMK S44D mutant expressed in HEK293 cells assessed as inhibition of thallium efflux incubated for 20 mins prior to thallium addition measured after 7 to 12 secs by fluorescence assay	Homo sapiens	1		cell-based format	Scientific Literature
4.	ALA2148639	B	Inhibition of ROMK		1		single protein format	Scientific Literature
5.	ALA1614332	F	PUBCHEM_BIOASSAY: High throughput discovery of novel modulators of ROMK K+ channel activity: Analog Library Testing. (Class of assay: confirmatory)	Homo sapiens	3		assay format	PubChem BioAssays
6.	ALA2411107	B	Inhibition of human Kir1.1 expressed in HEK293 cells by thallium flux assay	Homo sapiens	8		cell-based format	Scientific Literature
7.	ALA2411108	B	Inhibition of human Kir1.1 expressed in CHO cells by whole cell voltage clamp assay	Homo sapiens	8		cell-based format	Scientific Literature
8.	ALA2443478	B	Inhibition of human ROMK channel by electrophysiology assay	Homo sapiens	4		single protein format	Scientific Literature
9.	ALA2443481	B	Inhibition of human ROMK channel-mediated 86Rb+ flux expressed in CHO cells at 30 uM after 35 mins by scintillation counting analysis relative to control	Homo sapiens	13		cell-based format	Scientific Literature
10.	ALA2443483	B	Inhibition of human ROMK channel-mediated 86Rb+ flux expressed in CHO cells after 35 mins by scintillation counting analysis	Homo sapiens	33		cell-based format	Scientific Literature
11.	ALA3615682	B	Inhibition of ROMK (unknown origin) expressed in HEK293 cells by thallium flux assay	Homo sapiens	32		cell-based format	Scientific Literature
12.	ALA3615694	B	Inhibition of ROMK (unknown origin) expressed in HEK293 cells by electrophysiology assay	Homo sapiens	7		cell-based format	Scientific Literature
13.	ALA3626480	B	Inhibition of human Kir1.1 expressed in HEK293 cells by thallium flux assay	Homo sapiens	12		cell-based format	Scientific Literature
14.	ALA3706160	B	Electrophysiology Assay: Block of Kir1.1 (ROMK1) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391:85-100 (1981)) using the IonWorks.RTM. Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, Calif.). Chinese hamster ovary cells stably expressing Kir1.1 channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO.sub.2 incubator at 37.degree. C. Prior to an experiment, Kir1.1 expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 ml of Versene.TM. (Invitrogen 15040-066) for approximately 6 minutes at 37.degree. C. and suspended in 10 ml of bath solution containing (in mM): 150 NaCl, 10 KCl, 2.7 CaCl.sub.2, 0.5 MgCl.sub.2, 5 HEPES, pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 ml of bath solution and placed in the IonWorks.RTM. instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl.	Homo sapiens	17		cell-based format	BindingDB Database
15.	ALA3705830	B	Thallium Flux Assay: FluxOR Kit Components (Invitrogen F10017) FluxOR Reagent (Component A) FluxOR Assay Buffer (Component B)-10x Concentrate PowerLoad Concentrate (Component C)-100x Concentrate Probenecid (Component D)-Lyophilized sample is kept at -20 C. Water soluble, 100x after solubilization in 1 ml water. Store at 4 C. FluxOR Chloride-free Buffer (Component E)-5x Concentrate Potassium sulfate (K2SO4) Concentrate (Component F)-125 mM in water. Store at 4 C. Thallium sulfate (Tl2SO4) Concentrate (Component G)-50 mM in water. Store at 4 C. DMSO (dimethyl sulfoxide, Component H)-1 ml (100%) Reagent Preparation- FluxOR Working Solutions 1000x FluxOR Reagent: Reconstitute a vial of component A in 100 ul DMSO; Mix well; Store 10 ul aliquots at -20 C. 1x FluxOR Assay Buffer: Dilute Component B 10-fold with water; Adjust pH to 7.4 with Hepes/NaOH; Filter and store at 4 C.	Homo sapiens	9		single protein format	BindingDB Database
16.	ALA3797006	B	Inhibition of human ROMK expressed in CHO cells by whole-cell voltage clamp method	Homo sapiens	8		cell-based format	Scientific Literature
17.	ALA3811738	B	Inhibition of human Kir1.1 expressed in HEK293 cells after 30 mins by thallium flux assay	Homo sapiens	11		cell-based format	Scientific Literature
18.	ALA3869647	B	Inhibition of human ROMK expressed in CHO cells after 6 mins at -70 mV holding potential by electrophysiology method	Homo sapiens	28		cell-based format	Scientific Literature
19.	ALA3871115	B	Inhibition of human ROMK channel expressed in HEK293 cells after 30 mins by FLIPR based thallium flux assay	Homo sapiens	5		cell-based format	Scientific Literature
20.	ALA3871116	B	Inhibition of human ROMK1 channel expressed in CHO-DHFR cells after 35 mins by 86Rb+ efflux assay	Homo sapiens	1		cell-based format	Scientific Literature