#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2049731	F	Antagonist activity at NKKC1 expressed in human HEK293 cells assessed as [86Rb] uptake up to 100 uM after 30 mins by scintillation counting	Homo sapiens	2		cell-based format	Scientific Literature
2.	ALA2049737	F	Antagonist activity at NKKC1 expressed in human HEK293 cells assessed as [86Rb] uptake at 50 uM after 30 mins by scintillation counting	Homo sapiens	2		cell-based format	Scientific Literature
3.	ALA2049738	F	Antagonist activity at NKKC1 expressed in human HEK293 cells assessed as [86Rb] uptake after 30 mins by scintillation counting	Homo sapiens	2		cell-based format	Scientific Literature
4.	ALA3998798	B	Inhibition of NKCC1 in human primary bronchial epithelial cells assessed as effect on short-circuit current at 10 uM after 10 mins by voltage clamp method	Homo sapiens	2		assay format	Scientific Literature
5.	ALA4418145	B	Inhibition of NKCC1 in human bronchial epithelial cells assessed as effect on short-circuit current at 25 uM pre-incubated for 30 mins	Homo sapiens	1		assay format	Patent Bioactivity Data
6.	ALA4418164	B	Inhibition of human NKCC1 expressed in COS7 co-expressing EYFP-HIF assessed as effect on I(-) gradient at 25 uM by YFP-based fluorescence quenching assay	Homo sapiens	1		assay format	Patent Bioactivity Data
7.	ALA5040042	B	Inhibition of NKCC1 (unknown origin) transfected in HEK293 cells expressing mbYFPQS plasmid assessed as reduction in Cl- influx at 10 uM incubated for 30 mins by fluorescence multiplate reader analysis relative to control	Homo sapiens	10		cell-based format	Scientific Literature
8.	ALA5040043	B	Inhibition of NKCC1 (unknown origin) transfected in HEK293 cells expressing mbYFPQS plasmid assessed as reduction in Cl- influx at 100 uM incubated for 30 mins by fluorescence multiplate reader analysis relative to control	Homo sapiens	26		cell-based format	Scientific Literature
9.	ALA5040049	B	Inhibition of NKCC1 (unknown origin) transfected in HEK293 cells expressing mbYFPQS plasmid assessed as reduction in Cl- influx at 10 uM incubated for 30 mins by fluorescence multiplate reader analysis	Homo sapiens	22		cell-based format	Scientific Literature
10.	ALA5040056	B	Inhibition of NKCC1 (unknown origin) transfected in HEK293 cells expressing mbYFPQS plasmid assessed as reduction in Cl- influx at 100 uM incubated for 30 mins by fluorescence multiplate reader analysis	Homo sapiens	5		cell-based format	Scientific Literature
11.	ALA5231873	B	Inhibition of NKCC1 in human HT-29 cells assessed as inhibition of rubidium uptake incubated for 20 min by atomic absorption spectroscopy	Homo sapiens	2		cell-based format	Scientific Literature