#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3629950	Binding	Inhibition of human liver cathepsin B using Z-RR-AMC as substrate after 5 mins by fluorescence assay	Homo sapiens	15	ALA3627648	assay format	Scientific Literature
2.	ALA3632406	Binding	Inhibition of human cathepsin B using Cbz-Phe-Arg-AMC at 100 uM measured by fluorescence spectrophotometry	Homo sapiens	20	ALA3627659	single protein format	Scientific Literature
3.	ALA3705708	Binding	Enzyme Inhibition Assay: Determination of the enzymatic activity of the catalytic domain of human Cathepsin B. This protein is obtained as an inactive enzyme from Sigma, Wiesbaden, Germany (catalog No. C8571).	Homo sapiens	24	ALA3638984	single protein format	BindingDB Database
4.	ALA3705447	Binding	Biological Assay: Human liver Cathepsin B (Calbiochem) was pre-incubated with test compound at various concentrations for 5 minutes at 37C. The assay was initiated by addition of substrate Z-Arg-Arg-aminomethylcoumarin (Z-R-R-AMC Bacchem) and the final assay conditions were 1.1 nM cathepsin B, 60 uM Z-R-R-AMC, 126 mM sodium potassium phosphate pH 6.0 (Fisher), 0.3 mM EDTA (Omnipure), 2.7 mM DTT (Omnipure), 0.004% BRIJ 35 (Sigma), and 2.0% DMSO (Acros) in a final volume of 200 uL. Test compounds were serially diluted with DMSO and 0.01% BRIJ 35 to include a final concentration range of 20 uM to 10 pM. The reaction was monitored fluorometrically for 5 minutes at 37C. using black 96-well Corning 3686 assay microplates with a Thermo Fluoroskan Ascent FL microplate reader at excitation and emission filter wavelengths of 355 nm and 460 nm, respectively.	Homo sapiens	10	ALA3639226	assay format	BindingDB Database
5.	ALA3707685	Binding	In Vitro Inhibition Assay: In vitro inhibition assay using cathepsin.	Homo sapiens	21	ALA3638812	single protein format	BindingDB Database
6.	ALA3707676	Binding	Enzyme Inhibition Assay: Enzyme activity is measured by observing the increase in fluorescence intensity caused by cleavage of a peptide substrate containing a fluorophore whose emission is quenched in the intact peptide. Assay buffer: 100 mM potassium phosphate pH 6.5, EDTA-Na 5 mM, Triton X-100 0.001%, DTT 5 mM. Enzymes (all at 1 nM): human and mouse Cathepsin S, Cat K, Cat B, Cat L. Substrate (20 μM): Z-Val-Val-Arg-AMC, except for Cat K which uses Z-Leu-Arg-AMC (both from Bachem).Z=Benzyloxycarbonyl.AMC=7-Amino-4-Methyl-Coumarin. DTT=dithiothreitol. Final volume: 100 μL. Excitation 360 nm, Emission 465 nm.	Homo sapiens	62	ALA3638694	single protein format	BindingDB Database
7.	ALA3744221	Binding	Inhibition of recombinant cathepsin B (unknown origin) using Z-Leu-Arg-AMC fluorogenic substrate	Homo sapiens	3	ALA3739391	single protein format	Scientific Literature
8.	ALA3807026	Binding	Inhibition of human liver cathepsin B using Cbz-Arg-Arg-pNA as substrate at pH 6 incubated for 30 mins measured for 20 mins by photometrical analysis	Homo sapiens	66	ALA3804805	assay format	Scientific Literature
9.	ALA3807027	Binding	Inhibition of human cathepsin B	Homo sapiens	1	ALA3804805	single protein format	Scientific Literature
10.	ALA3807028	Binding	Inhibition of human liver cathepsin B using Cbz-Arg-Arg-pNA as substrate at pH 4.5 incubated for 30 mins measured for 20 mins by photometrical analysis	Homo sapiens	5	ALA3804805	assay format	Scientific Literature
11.	ALA3807029	Binding	Inhibition of human liver cathepsin B using Abz-Gly-Ile-Val-Arg-Ala-Lys(Dnp)-OH as substrate at pH 4.5 incubated for 30 mins measured for 10 mins by photometrical analysis	Homo sapiens	5	ALA3804805	assay format	Scientific Literature
12.	ALA3808086	Binding	Inhibition of human liver cathepsin B using Cbz-RR-AMC as substrate by fluorometric assay	Homo sapiens	4	ALA3804776	assay format	Scientific Literature
13.	ALA3808088	Binding	Inhibition of human liver cathepsin B using Cbz-RR-AMC as substrate at 100 uM by fluorometric assay	Homo sapiens	2	ALA3804776	assay format	Scientific Literature
14.	ALA661072	Binding	Compound is evaluated for inhibition kinetic constant (kobs/[I], 1/M.s) for cathepsin B		8	ALA1128689	single protein format	Scientific Literature
15.	ALA661073	Binding	Compound is evaluated for inhibition kinetic constant (kobs/[I], 1/M.s) for cathepsin B; No data		1	ALA1128689	single protein format	Scientific Literature
16.	ALA661074	Binding	Tested for inhibition of cathepsin B by the compound		1	ALA1149106	single protein format	Scientific Literature
17.	ALA661075	Binding	Tested for inhibition of cathepsin B by the compound; ND = Not determined		1	ALA1149106	single protein format	Scientific Literature
18.	ALA661076	Binding	Tested for inhibition of cathepsin B by the compound; NI = No Inhibition		6	ALA1149106	single protein format	Scientific Literature
19.	ALA661077	Binding	Tested for inhibition of cathepsin B; ND = Not determined		1	ALA1149106	single protein format	Scientific Literature
20.	ALA661078	Binding	Inhibition of cathepsin B; Inactive		3	ALA1145789	single protein format	Scientific Literature