# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1657524 | B | Inhibition of EGF-srimulated Stat1 phosphorylation in mouse NIH/3T3 cells overexpressing human EGFR at 40 uM after 15 to 60 mins by immunoblotting | Mus musculus | 2 | ALA1649103 | cell-based format | Scientific Literature | |
2. | ALA1687313 | B | Displacement of 5-carboxyfluorescein-GpYDKPHVL-NH2 from mouse recombinant His-tagged STAT1-SH2 domain expressed in Escherichia coli BL21 (DE3) by fluorescence polarization assay | Mus musculus | 2 | ALA1681633 | assay format | Scientific Literature | |
3. | ALA1832674 | B | Inhibition of mouse STAT1 using fluorescent probe 5-carboxyfluorescein-GpYLPQTV-NH2 after 30 mins by fluorescence polarisation assay | Mus musculus | 1 | ALA1828629 | single protein format | Scientific Literature | |
4. | ALA2065326 | B | Inhibition of STAT1 phosphorylation in LPS-induced mouse RAW264.7 cells incubated for 10 mins prior to LPS-challenge measured after 30 mins by Western blot analysis | Mus musculus | 3 | ALA2062365 | cell-based format | Scientific Literature | |
5. | ALA2175194 | B | Inhibition of STAT1 dimerization in EGF-stimulated mouse NIH/3T3 cells overexpressing human EGFR assessed as suppression of STAT1-DNA interaction after 30 mins by EMSA | Mus musculus | 1 | ALA2169778 | cell-based format | Scientific Literature | |
6. | ALA3128734 | B | Effect on LPS-induced nuclear translocation of STAT1 in mouse RAW264.7 cells at 25 uM preincubated for 15 mins followed by LPS challenge measured after 6 hrs by Western blot analysis | Mus musculus | 1 | ALA3124901 | cell-based format | Scientific Literature | |
7. | ALA3128736 | B | Effect on LPS-induced STAT1 phosphorylation in mouse RAW264.7 cells at 3.1 to 25 uM preincubated for 15 mins followed by LPS challenge measured after 4 hrs by Western blot analysis | Mus musculus | 1 | ALA3124901 | cell-based format | Scientific Literature | |
8. | ALA3632016 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | single protein format | Scientific Literature | |
9. | ALA3632018 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 12 | ALA3627612 | single protein format | Scientific Literature | |
10. | ALA3632141 | B | Modulation of EGF-induced Stat1 phosphorylation in mouse NIH3T3 cells expressing human EGFR at 5 uM preincubated for 3 hrs followed by stimulation with 1 ug/ml EGF for 12 mins by immunoblot analysis | Mus musculus | 4 | ALA3627612 | cell-based format | Scientific Literature | |
11. | ALA3743447 | B | Inhibition of IL6-induced STAT1 phosphorylation at Y701 in CD4+ T lymphocytes isolated from C57BL/6J mouse preincubated for 2 hrs followed by activation with plate-bound anti-CD3/anti-CD28 and IL6 for 15 mins by Western blot analysis | Mus musculus | 6 | ALA3739303 | cell-based format | Scientific Literature | |
12. | ALA4423956 | B | Inhibition of LPS-induced STAT1 phosphorylation at Y701 residue in mouse RAW264.7 cells at 5 uM preincubated for 30 mins followed by LPS-stimulation and measured up to 240 mins by Western blot analysis | Mus musculus | 1 | ALA4422643 | cell-based format | Scientific Literature | |
13. | ALA4423958 | B | Inhibition of LPS-induced STAT1 phosphorylation at Y701 residue in mouse RAW264.7 cells at 40 uM preincubated for 30 mins followed by LPS-stimulation and measured up to 240 mins by Western blot analysis | Mus musculus | 1 | ALA4422643 | cell-based format | Scientific Literature | |
14. | ALA4477559 | B | Inhibition of LPS-induced STAT1 phosphorylation at Y701 in mouse RAW264.7 cells at 15 to 60 uM preincubated for 1 hr followed by LPS challenge for 120 mins by Western blot analysis | Mus musculus | 1 | ALA4477178 | cell-based format | Scientific Literature | |
15. | ALA4477560 | B | Inhibition of LPS-induced STAT1 phosphorylation at S727 in mouse RAW264.7 cells at 15 to 60 uM preincubated for 1 hr followed by LPS challenge for 15 mins by Western blot analysis | Mus musculus | 1 | ALA4477178 | cell-based format | Scientific Literature | |
16. | ALA4477562 | B | Inhibition of LPS-induced STAT1 phosphorylation at Y701 in C57BL/6 mouse peritoneal macrophages at 15 to 60 uM preincubated for 1 hr followed by LPS challenge for 120 mins by Western blot analysis | Mus musculus | 1 | ALA4477178 | single protein format | Scientific Literature | |
17. | ALA4477563 | B | Inhibition of LPS-induced STAT1 phosphorylation at S727 in C57BL/6 mouse peritoneal macrophages at 15 to 60 uM preincubated for 1 hr followed by LPS challenge for 30 mins by Western blot analysis | Mus musculus | 1 | ALA4477178 | single protein format | Scientific Literature | |
18. | ALA4712509 | B | Inhibition of oligonucleotide [32P]-labelled 5'-AGCITCATTTCCCGTAAATCCCTA probe binding to STAT1 homodimer in mouse NIH3T3 nuclear extract preincubated for 30 mins followed by hSIE probe addition by EMSA analysis | Mus musculus | 5 | ALA4706639 | subcellular format | Scientific Literature | |
19. | ALA4712512 | B | Inhibition of oligonucleotide [32P]-labelled 5'-AGCITCATTTCCCGTAAATCCCTA probe binding to STAT1 homodimer in mouse NIH3T3 nuclear extract at 10 uM preincubated for 30 mins followed by hSIE probe addition by EMSA analysis | Mus musculus | 10 | ALA4706639 | tissue-based format | Scientific Literature | |
20. | ALA4723882 | B | Inhibition of STAT1 phosphorylation in mouse BAF3 cells at 1000 nM after 2 hrs by Western blot analysis | Mus musculus | 1 | ALA4715869 | cell-based format | Scientific Literature |