#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1681413	B	Displacement of [125I]-prolactin releasing peptide-20 from PrRPR at 10 uM after 30 mins		2	ALA1671829	single protein format	Scientific Literature
2.	ALA3089337	B	Antagonist activity at PRLHR (unknown origin) at 10 uM after 3 to 5 hrs by beta-arrestin assay relative to control	Homo sapiens	1	ALA3085628	single protein format	Scientific Literature
3.	ALA3089346	B	Agonist activity at PRLHR (unknown origin) at 10 uM after 90 to 180 mins by beta-arrestin assay relative to control	Homo sapiens	1	ALA3085628	single protein format	Scientific Literature
4.	ALA3734209	B	Displacement of [3H]-PrRP from human GPR10 receptor expressed in HEK293 cell membranes incubated for 90 mins by liquid scintillation counting method	Homo sapiens	88	ALA3727366	cell-based format	Patent Bioactivity Data
5.	ALA3734210	F	Antagonist activity against human GPR10 receptor expressed in HEK293 cells assessed as inhibition of PrRP-induced intracellular calcium mobilization pre-incubated for 15 mins before PrRP addition by Fluo-8 dye based fluorescence imaging plate reader method	Homo sapiens	49	ALA3727366	cell-based format	Patent Bioactivity Data
6.	ALA3887681	B	Inhibition Assay: Receptor-expressing HEK 293 cell membrane fraction was analyzed by modifying the methods of C. J. Langmead et al. (see Langmead C J, Szekeres P G; Chambers J K, Ratcliffe S J, Jones D N C, Hirst W D, et al. Characterization of the binding of [125I]-human prolactin releasing peptide (PrRP) to GPR10, a novel G protein coupled receptor. Br J Pharmacol 2000; 131: 683-688.). To each well of a 96-well plate, 150 μl of assay buffer (20 mM HEPES, 10 mM EDTA, 1 μl/ml protease inhibiting agent cocktail, pH 7.4), 20 μl of cell membrane fraction, 10 μl of test compound and [3H]-PrRP (final concentration 1 nM) were added and incubated at room temperature for 90 minutes. After completion of the reaction, using a cell harvester, the cell-membrane fraction sample product was filtered under aspiration by a glass fiber filter plate (Unifilter; GF/B) previously treated with 0.5% polyethylene imine. The filter was washed with a 50 mM Tris hydrochloric acid buffer (pH 7.4) three times.		88	ALA3886405	cell-based format	BindingDB Database
7.	ALA3887682	F	Intracellular Calcium Mobilization Assay: HEK293 cells expressing human GPR10 were maintained in Dulbecco's Modified Eagles' medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin and 20 mM HEPES at 37° C. in a 5% CO2 incubator. GPR10-expressing HEK293 cells were placed in poly-D-lysine coated 96-well culture plates and cultured for 18-24 hr before the test at a density of 3×104 cells/well. The cells were incubated with 2.5 nM Fluo-8 for 1 hr at room temperature in Recording Buffer containing 1% bovine serum albumin, 0.01% pluronic F-127 and 20 mM HEPES, Hanks Balanced Buffered Saline, pH 7.4. The cells were incubated with test compounds for 15 min at room temperature, and then PrRP (the final concentration, 1 nM) was added into the medium. The changes in intracellular calcium-dependent fluorescence were monitored using a fluorescence imaging plate reader (FDSS3000, Hamamatsu Photonics K.K.). Fluo-8 fluorescence was measured with excitation at 490 nm and emission at 520 nm.		49	ALA3886405	cell-based format	BindingDB Database
8.	ALA4312696	B	Activity at human PRP receptor at 10 uM	Homo sapiens	1	ALA4311943	single protein format	Scientific Literature
9.	ALA4344224	B	Antagonist activity at human Gq-coupled PRLHR expressed in CHOK1 cells assessed as inhibition in prolactin releasing peptide (1 to 31)-induced beta-arrestin 2 recruitment at 20 uM incubated for 30 mins followed by prolactin releasing peptide (1 to 31) addition and measured after 90 or 180 mins by pathhunter beta-arrestin assay relative to control	Homo sapiens	9	ALA4342456	cell-based format	Scientific Literature
10.	ALA4344230	B	Antagonist activity at human Gq-coupled PRLHR expressed in CHOK1 cells assessed as inhibition in prolactin releasing peptide (1 to 31)-induced beta-arrestin 2 recruitment incubated for 30 mins followed by prolactin releasing peptide (1 to 31) addition and measured after 90 or 180 mins by pathhunter beta-arrestin assay	Homo sapiens	2	ALA4342456	cell-based format	Scientific Literature
11.	ALA4700945	F	Activation of human GPR10 overexpressed in CHO-K1 cells assessed as increase in beta arrestin recruitment incubated for 90 mins in presence of 10% FBS by PathHunter assay	Homo sapiens	9	ALA4699444	cell-based format	Scientific Literature
12.	ALA4700946	F	Ratio of EC50 for activation of human GPR10 overexpressed in CHO-K1 cells assessed as increase in beta arrestin recruitment incubated for 90 mins in presence of 10% FBS by PathHunter assay to EC50 for activation of human GPR10 overexpressed in CHO-K1 cells assessed as increase in beta arrestin recruitment incubated for 90 mins by PathHunter assay	Homo sapiens	9	ALA4699444	cell-based format	Scientific Literature
13.	ALA4700961	F	Activation of human GPR10 overexpressed in CHO-K1 cells assessed as increase in beta arrestin recruitment incubated for 90 mins by PathHunter assay	Homo sapiens	122	ALA4699444	cell-based format	Scientific Literature
14.	ALA4726260	B	Antagonist activity at human PRLHR expressed in CHO-K1 cells at 20 uM by PathHunter beta-arrestin assay relative to control	Homo sapiens	8	ALA4725259	cell-based format	Scientific Literature
15.	ALA4726264	B	Antagonist activity at human PRLHR expressed in CHO-K1 cells by PathHunter beta-arrestin assay	Homo sapiens	1	ALA4725259	cell-based format	Scientific Literature
16.	ALA4726287	B	Agonist activity at human PRLHR expressed in CHO-K1 cells at 20 uM by PathHunter beta-arrestin assay relative to control	Homo sapiens	8	ALA4725259	cell-based format	Scientific Literature
17.	ALA4772290	B	Displacement of [3H]-PrRP-20 from human PrRP receptor expressed in CHO cell membrane by TopCount scintillation counter method	Homo sapiens	2	ALA4765381	cell membrane format	Scientific Literature
18.	ALA4883576	B	PRESTO-Tango GPCRome screening (PRRP)		1	ALA4507309	single protein format	Donated Chemical Probes - SGC Frankfurt