#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3707519	Binding	Enzymatic Assay: Cyclophilin PPlase activity was measured at 20 C. by using the standard chymotrypsin coupled assay (Kofron J L, Kuzmic P, Kishore V, Colon-Bonilla E, Rich D H. Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay. Biochemistry. 1991 Jun. 25; 30(25):6127-34). The assay buffer (25 mM Hepes, 100 mM NaCl, pH 7.8) and CypA, B or D (1900 nM stock solution) were pre-cooled to 4 C., to which then was added 5 uL of 50 mg/ml chymotrypsin in 1 mM HCl. The reaction was initiated by adding 20 uL of 3.2 mM peptide substrate (Suc-Ala-Ala-cis-Pro-Phe-pNA) in LiCl/TFE solution with rapid inversion. After a delay from the onset of mixing, the absorbance of p-nitroaniline was followed at 390 nM until the reaction was complete (1 min). The final concentration of LiCl in the assay was 20 mM; TFE was present at a concentration of 4% (v/v). Absorbance readings were collected every 1 s by spectrophotometer.	Homo sapiens	10	ALA3639117	single protein format	BindingDB Database
2.	ALA3801393	Binding	Inhibition of Cyclophilin D (unknown origin) activity preincubated for 15 mins followed Suc-AAPF-pNA substrate addition by chymotrypsin coupled based spectrophotometry assay	Homo sapiens	17	ALA3797189	single protein format	Scientific Literature
3.	ALA3801395	Binding	Binding affinity to Cyclophilin D (unknown origin) by isothermal titration calorimetry	Homo sapiens	17	ALA3797189	single protein format	Scientific Literature
4.	ALA3801396	Binding	Inhibition of Cyclophilin D (unknown origin) activity in absence of detergent	Homo sapiens	1	ALA3797189	single protein format	Scientific Literature
5.	ALA3808128	Binding	Binding affinity to human recombinant cyclophilin D by surface plasmon resonance analysis	Homo sapiens	2	ALA3804783	single protein format	Scientific Literature
6.	ALA3808129	Binding	Inhibition of human recombinant cyclophilin D using Suc-AAPF-MCA as substrate preincubated for 1 hr followed by substrate addition measured per millisec for 2 mins by real time fluorescence analysis	Homo sapiens	2	ALA3804783	single protein format	Scientific Literature
7.	ALA3808237	Binding	Inhibition of cyclophilin D in human SK-N-SH cells assessed as reversal of Abeta (1 to 42 residues) mediated reduction of cytochrome c oxidase activity at 10 to 20 uM after 48 hrs by spectrophotometrical analysis relative to vehicle control	Homo sapiens	1	ALA3804783	cell-based format	Scientific Literature
8.	ALA3808239	Binding	Inhibition of cyclophilin D in human SK-N-SH cells assessed as reversal of Abeta (1 to 42 residues) mediated decrease in ATP level after 48 hrs by bioluminescence assay	Homo sapiens	1	ALA3804783	cell-based format	Scientific Literature
9.	ALA3808241	Binding	Inhibition of cyclophilin D in human SK-N-SH cells assessed as inhibition of Abeta (1 to 42 residues) mediated cell death at 10 to 20 uM after 48 hrs by MTT assay	Homo sapiens	1	ALA3804783	cell-based format	Scientific Literature
10.	ALA4364106	Binding	Binding affinity to CypD (unknown origin) assessed as dissociation constant by SPR analysis	Homo sapiens	2	ALA4359930	single protein format	Scientific Literature
11.	ALA4364108	Binding	Displacement of fluorescein labelled cyclosporine A derivative from human recombinant CypD (30 to 207 residues) expressed in Escherichia coli cells incubated for 90 mins by fluorescence polarization assay	Homo sapiens	23	ALA4359930	cell-based format	Scientific Literature
12.	ALA4364109	Binding	Inhibition of CypD (unknown origin) PPIase activity using N-Suc-AAPF-p-nitroanilide as substrate preincubated with enzyme for 10 mins followed by chymotrypsin addition and further incubation for 4 mins followed by substrate addition	Homo sapiens	23	ALA4359930	single protein format	Scientific Literature
13.	ALA4364111	Binding	Potency index, ratio of IC50 of (R)-1-(4-aminobenzyl)-3-(2-(2-(2-(methylthio)phenyl)pyrrolidin-1-yl)-2-oxoethyl)urea to IC50 of compound for human recombinant CypD (30 to 207 residues) expressed in Escherichia coli cells incubated for 90 mins by fluorescence polarization assay	Homo sapiens	1	ALA4359930	cell-based format	Scientific Literature
14.	ALA1696288	Binding	Inhibition of Cyclophilin 40 PPIase activity		2	ALA1687693	single protein format	Scientific Literature
15.	ALA1696290	Binding	Inhibition of cyclosporin binding to Cyp40 by fluorescence polarization competition assay		1	ALA1687693	single protein format	Scientific Literature
16.	ALA1696293	Binding	Inhibition of fluorescein labeled cyclosporin binding to Cyp40 by fluorescence polarization competition assay		1	ALA1687693	single protein format	Scientific Literature
17.	ALA5253383	Binding	Binding affinity to CypD (unknown origin)	Homo sapiens	3	ALA5252525	single protein format	Scientific Literature
18.	ALA5253387	Binding	Inhibition of CypD (unknown origin)	Homo sapiens	2	ALA5252525	single protein format	Scientific Literature