# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1697042 | B | Inhibition of protease activity of recombinant Clostridium botulinum BoNT/E light chain expressed in Escherichia coli (pET24a+/BL21(DE3)) assessed as cleavage of SNAP-25 at 240 uM after 30 mins by SDS-PAGE analysis | Clostridium botulinum | 5 | ALA1687736 | assay format | Scientific Literature | |
2. | ALA3830808 | B | Inhibition of endopeptidase activity of Clostridium botulinum BoNT/E light chain using SNAP-25 as substrate at 20 uM after 15 mins by HPLC analysis | Clostridium botulinum | 37 | ALA3826967 | single protein format | Scientific Literature | |
3. | ALA3830809 | B | Inhibition of endopeptidase activity of Clostridium botulinum BoNT/E light chain using SNAP-25 as substrate after 15 mins by HPLC analysis | Clostridium botulinum | 22 | ALA3826967 | single protein format | Scientific Literature | |
4. | ALA3830810 | B | Inhibition of protease activity of Clostridium botulinum BoNT/E light chain using SNAP-25 as substrate after 15 mins by Dixon plot analysis | Clostridium botulinum | 1 | ALA3826967 | single protein format | Scientific Literature | |
5. | ALA4432669 | B | Inhibition of Clostridium botulinum BoNT/E light chain-mediated SNAP-25 cleavage assessed as reduction in residual activity using SNAP-Etide as substrate at 100 uM after 15 mins by micro plate reader method | Clostridium botulinum | 9 | ALA4431362 | single protein format | Scientific Literature | |
6. | ALA4432670 | B | Inhibition of Clostridium botulinum BoNT/E light chain using GFP-tagged VAMP2/SNAP-25 (Ala128 to Gly206 residues) as substrate at 100 uM after 15 mins by GFP based micro plate reader method | Clostridium botulinum | 2 | ALA4431362 | single protein format | Scientific Literature | |
7. | ALA4432671 | B | Inhibition of Clostridium botulinum BoNT/E light chain using GFP-tagged VAMP2/SNAP-25 (Ala128 to Gly206 residues) as substrate at 25 uM after 15 mins by GFP based micro plate reader method | Clostridium botulinum | 2 | ALA4431362 | single protein format | Scientific Literature | |
8. | ALA4432672 | B | Inhibition of Clostridium botulinum BoNT/E light chain using GFP-tagged VAMP2/SNAP-25 (Ala128 to Gly206 residues) as substrate at 12.5 uM after 15 mins by GFP based micro plate reader method | Clostridium botulinum | 2 | ALA4431362 | single protein format | Scientific Literature | |
9. | ALA4432673 | B | Inhibition of Clostridium botulinum BoNT/E light chain using GFP-tagged VAMP2/SNAP-25 (Ala128 to Gly206 residues) as substrate after 15 mins by GFP based micro plate reader method | Clostridium botulinum | 11 | ALA4431362 | single protein format | Scientific Literature | |
10. | ALA4432677 | B | Inhibition of protease activity of Clostridium botulinum BoNT/E using SNAP-25 as substrate after 15 mins by HPLC based assay | Clostridium botulinum | 1 | ALA4431362 | single protein format | Scientific Literature | |
11. | ALA4432679 | B | Potency index, ratio of IC50 for NSC-77053 to IC50 for test compound for inhibition of Clostridium botulinum BoNT/E light chain using GFP-tagged VAMP2/SNAP-25 (Ala128 to Gly206 residues) as substrate after 15 mins by GFP based micro plate reader method | Clostridium botulinum | 1 | ALA4431362 | single protein format | Scientific Literature |