| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1737963 | F | PUBCHEM_BIOASSAY: Dose Response confirmation of uHTS for the identification of inhibitors of NALP3 in yeast using a luminescent assay. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2825, AID2832] | Homo sapiens | 69 | assay format | PubChem BioAssays | ||
| 2. | ALA1738451 | F | PUBCHEM_BIOASSAY: Dose Response confirmation of inhibitors of NALP3 in yeast using a Caspase-1-ASC counter screen. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2825, AID2832] | Homo sapiens | 67 | assay format | PubChem BioAssays | ||
| 3. | ALA5140076 | B | Binding affinity to human recombinant full length NLRP3 transfected in HEK293F cells assessed as dissociation constant incubated for 40 mins by microscale thermophoresis analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA3390772 | B | Inhibition of recombinant NLRP3-ATPase (unknown origin) assessed as hydrolysis of ATP at 10 uM | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 5. | ALA3784286 | B | Inhibition of NLRP3 in LPS activated human monocytes after 30 mins by ELISA analysis in presence of ATP | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3784288 | B | Reversible inhibition of NLRP3 in LPS activated human monocytes after 30 mins by ELISA analysis in presence of ATP | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 7. | ALA3784389 | B | Inhibition of NLRP3 in LPS stimulated human THP1 cells assessed as reduction in IL1beta release after 2 hrs by ELISA analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3864034 | B | Inhibition of NLRP3 inflammasome in LPS-stimulated human monocyte derived macrophages assessed as reduction in NLRP3 inflammasome-induced IL-1beta production preincubated for 30 mins followed by NLRP3 stimulation with ATP for 1 hr by ELISA | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 9. | ALA4015426 | B | Inhibition of recombinant human NLRP3 ATPase assessed as hydrolysis of ATP at 100 uM preincubated for 15 mins followed by addition of ATP measured after 40 mins by ADP-Glo assay | Homo sapiens | 16 | single protein format | Scientific Literature | ||
| 10. | ALA4015427 | B | Inhibition of recombinant human NLRP3 ATPase assessed as hydrolysis of ATP at 50 uM preincubated for 15 mins followed by addition of ATP measured after 40 mins by ADP-Glo assay | Homo sapiens | 16 | single protein format | Scientific Literature | ||
| 11. | ALA4015428 | B | Inhibition of NLRP3 in PMA-differentiated and LPS-primed human THP1 cells assessed as decrease in ATP-induced pyroptosis measured as LDH activity at 10 uM preincubated for 1 hr followed by ATP addition measured after 1 hr by colorimetric assay relative to control | Homo sapiens | 13 | assay format | Scientific Literature | ||
| 12. | ALA4015445 | B | Inhibition of recombinant YFP-tagged NLRP3 (unknown origin) expressed in HEK293 cells co-expressing luciferase at 10 to 100 uM after 24 hrs by BRET assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4015447 | B | Inhibition of recombinant YFP-tagged NLRP3 (unknown origin) expressed in HEK293 cells co-expressing luciferase assessed as reduction in nigericin-induced intracellular K+ decrease-mediated conformational changes in protein at 10 to 100 uM measured after 24 hrs by BRET assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4015448 | B | Inhibition of recombinant YFP-tagged NLRP3 (unknown origin) expressed in HEK293 cells co-expressing luciferase assessed as initial conformational changes in protein by measuring increase in nigericin-induced decrease in intracellular K+ level at 10 to 100 uM measured after 24 hrs by BRET assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4015449 | B | Irreversible inhibition of His-FLAG- tagged NLRP3-ATPase (unknown origin) at 100 uM preincubated for 55 mins followed by ATP addition measured after 40 mins by ADP-Glo assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 16. | ALA4015450 | B | Irreversible inhibition of His-FLAG- tagged NLRP3-ATPase (unknown origin) at 100 uM preincubated for 55 mins followed by compound wash-out and ATP addition measured after 40 mins by ADP-Glo assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 17. | ALA4015486 | B | Inhibition of recombinant human NLRP3 ATPase assessed as hydrolysis of ATP preincubated for 15 mins followed by addition of ATP measured after 40 mins by ADP-Glo assay | Homo sapiens | 10 | single protein format | Scientific Literature | ||
| 18. | ALA4026427 | A | Agonist activity at NLRP3 in human THLP1-null cells assessed as induction of LPS-induced IL-1beta secretion by HEK-Blue reporter cell based assay | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
| 19. | ALA4125669 | B | Inhibition of nigericin-induced NLRP3 inflammasome activation in PMA-differentiated human THP1 cells assessed as reduction in LPS-stimulated IL-1beta secretion incubated for 1 hr prior to stimulation with nigericin for 1 hr by quanti-blue based SEAP reporter gene assay | Homo sapiens | 9 | assay format | Scientific Literature | ||
| 20. | ALA4125672 | B | Inhibition of nigericin-induced NLRP3 inflammasome activation in PMA-differentiated human THP1 cells assessed as reduction in LPS-stimulated IL-1beta secretion at 60 mM incubated for 1 hr prior to stimulation with nigericin for 1 hr by ELISA | Homo sapiens | 1 | assay format | Scientific Literature |
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