#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1737963	F	PUBCHEM_BIOASSAY: Dose Response confirmation of uHTS for the identification of inhibitors of NALP3 in yeast using a luminescent assay. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2825, AID2832]	Homo sapiens	69	ALA1201862	assay format	PubChem BioAssays
2.	ALA1738451	F	PUBCHEM_BIOASSAY: Dose Response confirmation of inhibitors of NALP3 in yeast using a Caspase-1-ASC counter screen. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2825, AID2832]	Homo sapiens	67	ALA1201862	assay format	PubChem BioAssays
3.	ALA3390772	B	Inhibition of recombinant NLRP3-ATPase (unknown origin) assessed as hydrolysis of ATP at 10 uM	Homo sapiens	5	ALA3351787	single protein format	Scientific Literature
4.	ALA3784286	B	Inhibition of NLRP3 in LPS activated human monocytes after 30 mins by ELISA analysis in presence of ATP	Homo sapiens	1	ALA3779837	cell-based format	Scientific Literature
5.	ALA3784288	B	Reversible inhibition of NLRP3 in LPS activated human monocytes after 30 mins by ELISA analysis in presence of ATP	Homo sapiens	2	ALA3779837	cell-based format	Scientific Literature
6.	ALA3784389	B	Inhibition of NLRP3 in LPS stimulated human THP1 cells assessed as reduction in IL1beta release after 2 hrs by ELISA analysis	Homo sapiens	1	ALA3779837	cell-based format	Scientific Literature
7.	ALA3864034	B	Inhibition of NLRP3 inflammasome in LPS-stimulated human monocyte derived macrophages assessed as reduction in NLRP3 inflammasome-induced IL-1beta production preincubated for 30 mins followed by NLRP3 stimulation with ATP for 1 hr by ELISA	Homo sapiens	5	ALA3862040	single protein format	Scientific Literature
8.	ALA4015426	B	Inhibition of recombinant human NLRP3 ATPase assessed as hydrolysis of ATP at 100 uM preincubated for 15 mins followed by addition of ATP measured after 40 mins by ADP-Glo assay	Homo sapiens	16	ALA4014309	single protein format	Scientific Literature
9.	ALA4015427	B	Inhibition of recombinant human NLRP3 ATPase assessed as hydrolysis of ATP at 50 uM preincubated for 15 mins followed by addition of ATP measured after 40 mins by ADP-Glo assay	Homo sapiens	16	ALA4014309	single protein format	Scientific Literature
10.	ALA4015428	B	Inhibition of NLRP3 in PMA-differentiated and LPS-primed human THP1 cells assessed as decrease in ATP-induced pyroptosis measured as LDH activity at 10 uM preincubated for 1 hr followed by ATP addition measured after 1 hr by colorimetric assay relative to control	Homo sapiens	13	ALA4014309	assay format	Scientific Literature
11.	ALA4015445	B	Inhibition of recombinant YFP-tagged NLRP3 (unknown origin) expressed in HEK293 cells co-expressing luciferase at 10 to 100 uM after 24 hrs by BRET assay	Homo sapiens	1	ALA4014309	cell-based format	Scientific Literature
12.	ALA4015447	B	Inhibition of recombinant YFP-tagged NLRP3 (unknown origin) expressed in HEK293 cells co-expressing luciferase assessed as reduction in nigericin-induced intracellular K+ decrease-mediated conformational changes in protein at 10 to 100 uM measured after 24 hrs by BRET assay	Homo sapiens	1	ALA4014309	cell-based format	Scientific Literature
13.	ALA4015448	B	Inhibition of recombinant YFP-tagged NLRP3 (unknown origin) expressed in HEK293 cells co-expressing luciferase assessed as initial conformational changes in protein by measuring increase in nigericin-induced decrease in intracellular K+ level at 10 to 100 uM measured after 24 hrs by BRET assay	Homo sapiens	1	ALA4014309	cell-based format	Scientific Literature
14.	ALA4015449	B	Irreversible inhibition of His-FLAG- tagged NLRP3-ATPase (unknown origin) at 100 uM preincubated for 55 mins followed by ATP addition measured after 40 mins by ADP-Glo assay	Homo sapiens	2	ALA4014309	single protein format	Scientific Literature
15.	ALA4015450	B	Irreversible inhibition of His-FLAG- tagged NLRP3-ATPase (unknown origin) at 100 uM preincubated for 55 mins followed by compound wash-out and ATP addition measured after 40 mins by ADP-Glo assay	Homo sapiens	2	ALA4014309	single protein format	Scientific Literature
16.	ALA4015486	B	Inhibition of recombinant human NLRP3 ATPase assessed as hydrolysis of ATP preincubated for 15 mins followed by addition of ATP measured after 40 mins by ADP-Glo assay	Homo sapiens	10	ALA4014309	single protein format	Scientific Literature
17.	ALA4026427	A	Agonist activity at NLRP3 in human THLP1-null cells assessed as induction of LPS-induced IL-1beta secretion by HEK-Blue reporter cell based assay	Homo sapiens	11	ALA4024746	cell-based format	Scientific Literature
18.	ALA4125669	B	Inhibition of nigericin-induced NLRP3 inflammasome activation in PMA-differentiated human THP1 cells assessed as reduction in LPS-stimulated IL-1beta secretion incubated for 1 hr prior to stimulation with nigericin for 1 hr by quanti-blue based SEAP reporter gene assay	Homo sapiens	9	ALA4118290	assay format	Scientific Literature
19.	ALA4125672	B	Inhibition of nigericin-induced NLRP3 inflammasome activation in PMA-differentiated human THP1 cells assessed as reduction in LPS-stimulated IL-1beta secretion at 60 mM incubated for 1 hr prior to stimulation with nigericin for 1 hr by ELISA	Homo sapiens	1	ALA4118290	assay format	Scientific Literature
20.	ALA4125673	B	Inhibition of nigericin-induced NLRP3 inflammasome activation in PMA-differentiated human THP1 cells assessed as reduction in LPS-stimulated processing of pro-IL-1beta to mature IL-1beta in supernatant at 150 uM incubated for 1 hr followed by nigericin addition measured after 1 hr by Western blot analysis	Homo sapiens	1	ALA4118290	assay format	Scientific Literature