| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1794430 | F | PUBCHEM_BIOASSAY: Dose response confirmation of the uHTS fluorescent assay for identification of inhibitors of ATG4B Set 2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID504462, AID504475] | 16 | assay format | PubChem BioAssays | |||
| 2. | ALA1738670 | F | PUBCHEM_BIOASSAY: Dose response confirmation of the uHTS fluorescent assay for identification of inhibitors of ATG4B. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID504462, AID504475] | Homo sapiens | 318 | assay format | PubChem BioAssays | ||
| 3. | ALA5256466 | B | Inhibition of N-terminal His6/SUMO-tagged full length human ATG4B expressed in Escherichia coli BL21 (DE3) cells using His-LC3B-GST as substrate preincubated with enzyme for 1 hr followed by substrate addition for 1 hr by HTRF assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA5256467 | B | Inhibition of N-terminal His6/SUMO-tagged full length human ATG4B expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in substrate cleavage using His-LC3B-GST as substrate preincubated with enzyme for 1 hr followed by substrate addition for 30 mins by gel electrophoresis analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA5256468 | B | Binding affinity to N-terminal His6/SUMO-tagged full length human ATG4B expressed in Escherichia coli BL21 (DE3) cells at 100 uM by CPMG spectroscopy | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA5256469 | B | Binding affinity to N-terminal His6/SUMO-tagged full length human ATG4B expressed in Escherichia coli BL21 (DE3) cells by SPR assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA5250365 | B | Inhibition of ATG4B (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA3870644 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 4 mM preincubated for 15 mins followed by LC3B-GST substrate addition after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA3870645 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 5 mM preincubated for 15 mins followed by LC3B-GST substrate addition after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA3870646 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 20 mM using LC3B-GST as substrate measured after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA3870647 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 40 mM using LC3B-GST as substrate measured after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA3870648 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 20 mM using LC3B-GST as substrate preincubated up to 20 mins followed by substrate addition measured after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA3870649 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli using LC3B-GST as substrate after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA3870650 | B | Inhibition of wild type recombinant Atg4B (unknown origin) expressed in Escherichia coli BL21 DE3 using N-terminal His6-tagged LC3B-PLA2 as substrate after 60 mins by NBD-C6-HPC dye-based fluorescence assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 15. | ALA3870651 | B | Inhibition of Atg4B (unknown origin) using YFP-LC3B-EmGFP as substrate after 40 mins by FRET-based assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 16. | ALA3870652 | B | Inhibition of Atg4B (unknown origin) using LC3B-GST as substrate preincubated for 24 hrs followed by substrate addition measured for 3 mins by SDS-PAGE assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA3870653 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 500 uM using LC3B-GST as substrate after 6 mins by SDS-PAGE assay | Homo sapiens | 184 | assay format | Scientific Literature | ||
| 18. | ALA3870654 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli assessed as uncleaved substrate level at 500 uM using LC3B-GST as substrate after 6 mins by SDS-PAGE assay | Homo sapiens | 178 | assay format | Scientific Literature | ||
| 19. | ALA3870655 | B | Competitive inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli at 500 uM using LC3B-GST as substrate after 6 mins by SDS-PAGE assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 20. | ALA3870656 | B | Inhibition of human recombinant GST-tagged Atg4B expressed in Escherichia coli after 2 to 20 hrs by fluorescence assay | Homo sapiens | 2 | assay format | Scientific Literature |
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