# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1228363 | B | Inhibition of eEF2-mediated tRNA translocation assessed as inhibition of polyphenylalanine synthesis at 200 uM after 10 mins by scintillation counting | 1 | ALA1221230 | single protein format | Scientific Literature | ||
2. | ALA1228366 | B | Inhibition of eEF2-mediated tRNA translocation assessed as inhibition of phenylalanyl puromycin formation | 2 | ALA1221230 | single protein format | Scientific Literature | ||
3. | ALA1228367 | B | Inhibition of eEF2-mediated tRNA translocation assessed as decrease in tripeptide (Met-Phe-Phe) formation by TLC | 2 | ALA1221230 | single protein format | Scientific Literature | ||
4. | ALA1228368 | B | Inhibition of eEF2-mediated tRNA translocation assessed as induction of dipeptide accumulation by TLC | 2 | ALA1221230 | single protein format | Scientific Literature | ||
5. | ALA1806257 | B | Inhibition of EFK2 assessed as residual enzyme activity at 10 uM | 1 | ALA1800061 | single protein format | Scientific Literature | ||
6. | ALA4033187 | B | Downregulation of EF2 phosphorylation in human MCF7 cells at 1.5 uM measured up to 36 hrs by Western blot analysis | Homo sapiens | 2 | ALA4028911 | cell-based format | Scientific Literature | |
7. | ALA4033188 | B | Downregulation of EF2 phosphorylation in human MDA-MB-231 cells at 3 uM measured up to 36 hrs by Western blot analysis | Homo sapiens | 2 | ALA4028911 | cell-based format | Scientific Literature | |
8. | ALA4033236 | B | Effect on EF2 phosphorylation in human MCF7 cells at 10 uM measured up to 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4028911 | cell-based format | Scientific Literature | |
9. | ALA4033237 | B | Effect on EF2 phosphorylation in human MDA-MB-231 cells at 10 uM measured up to 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4028911 | cell-based format | Scientific Literature | |
10. | ALA4270831 | B | Binding affinity to elongation factor 2 in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis | Homo sapiens | 1 | ALA4270485 | cell-based format | Scientific Literature | |
11. | ALA4270833 | B | Binding affinity to eukaryotic translation initiation factor in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis | Homo sapiens | 1 | ALA4270485 | cell-based format | Scientific Literature | |
12. | ALA5145240 | B | Binding affinity to elongation factor 2 in human MDA-MB-453 cells measured after 6 hrs by pull-down assay | Homo sapiens | 1 | ALA5143564 | cell-based format | Scientific Literature | |
13. | ALA5145303 | B | Binding affinity to elongation factor 2 in human DOHH-2 cells measured after 6 hrs by pull-down assay | Homo sapiens | 1 | ALA5143564 | cell-based format | Scientific Literature | |
14. | ALA5145329 | B | Binding affinity to elongation factor 2 in human DOHH-2 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay | Homo sapiens | 1 | ALA5143564 | cell-based format | Scientific Literature | |
15. | ALA5145346 | B | Binding affinity to elongation factor 2 in human MDA-MB-453 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay | Homo sapiens | 1 | ALA5143564 | cell-based format | Scientific Literature | |
16. | ALA5145363 | B | Binding affinity to elongation factor 2 in human DOHH-2 cells by MS analysis | Homo sapiens | 1 | ALA5143564 | cell-based format | Scientific Literature | |
17. | ALA5166161 | B | Binding affinity to EEF2 in human NCI-H338 cell lysate assessed as trypsin-mediated biotinylated cysteine residues digestion by measuring reduction in protein level at 4 uM pretreated for 2 hrs followed by labelled with iodoacetamide-desthiobiotin by MS analysis relative to control | Homo sapiens | 1 | ALA5154891 | subcellular format | Scientific Literature |