| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1804753 | B | Inhibition of PRMT5-mediated methylation of histone H4 at 100 uM after 90 mins by scintillation counting in presence of S-adenosyl-l-[methyl-3H]methionine | 2 | single protein format | Scientific Literature | |||
| 2. | ALA2050393 | B | Inhibition of His6X-tagged PRMT5 expressed in Escherichia coli BL21(DE3) cells using [14C]-S-adenosyl-L-methionine and histone H4 peptide after 8 mins by scintillation counting | 1 | cell-based format | Scientific Literature | |||
| 3. | ALA2175624 | B | Inhibition of His6x-tagged PRMT5 expressed in Escherichia coli BL21 (DE3) using Histone H4(1-20 substrate in the presence of [14C] SAM assessed as residual activity at 30 uM by liquid scintillation counting | 1 | assay format | Scientific Literature | |||
| 4. | ALA2209107 | B | Inhibition of PRMT5 using histone H3 as substrate preincubated for 10 mins followed by addition of [3H]SAM and incubated for 60 mins | 3 | single protein format | Scientific Literature | |||
| 5. | ALA2330204 | B | Inhibition of PRMT5-MEP5 complex (unknown origin) using histone H4 (1 to 24) as substrate after 1 hr by scintillation proximity assay in presence of [3H]-S-adenosylmethionine | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA2350643 | B | Inhibition of PRMT5 (unknown origin) using [3H]SAM after 1 hr by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA3112256 | B | Inhibition of recombinant human PRMT5 expressed in Sf9 cells using histone H4 peptide as substrate at 100 uM by chemiluminescence assay | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
| 8. | ALA3112257 | B | Inhibition of recombinant human PRMT5 expressed in Sf9 cells using histone H4 peptide as substrate by chemiluminescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 9. | ALA3239250 | B | Inhibition of HA-tagged recombinant PRMT5 (unknown origin) expressed in HEK293T cells using [3H]SAM and histone H4 (1 to 20) as substrate at 20 uM after 8 mins by P81 filter binding assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 10. | ALA3239252 | B | Inhibition of HA-tagged recombinant PRMT5 (unknown origin) expressed in HEK293T cells using [3H]SAM and histone H4 (1 to 20) as substrate after 8 mins by P81 filter binding assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 11. | ALA3378208 | B | Inhibition of PRMT5 (unknown origin) assessed as incorporation of tritium-labeled methyl group to lysine or arginine residues of peptide substrate by radioactive assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA3411809 | B | Displacement of [3H]-SAM from recombinant His6-tagged PRMT5 (unknown origin) expressed in Escherichia coli BL21(DE3) incubated for 5 mins prior to H4(1 to 20)-BTN peptide addition measured after 8 mins by scintillation proximity assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 13. | ALA3578931 | B | Inhibition of human full length PRMT5 expressed in Sf9 cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 14. | ALA3636208 | B | Inhibition of PRMT5 (unknown origin) using biotinylated histone H4-derived peptide as substrate after 60 mins by AlphaLISA assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA3784630 | B | Inhibition of human PRMT5 using 24 residues of biotin labelled histone4 substrate and tritiated 3H-S-adenosylmethionine by scintillation proximity assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 16. | ALA3821900 | B | Inhibition of PRMT5 (unknown origin) at 1 to 50 uM by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA3859047 | B | Inhibition of PRMT5 (unknown origin) assessed as inhibition of methylation activity measured after 3 hrs using [3H]-SAM by scintillation proximity assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 18. | ALA3880378 | B | Inhibition of PRMT5 in human Z138 cells assessed as decrease in di-methyl arginine levels after 96 hrs by Western blot method | Homo sapiens | 331 | cell-based format | Patent Bioactivity Data | ||
| 19. | ALA3994772 | B | Inhibition of PRMT5 (unknown origin) | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 20. | ALA4006083 | B | Inhibition of PRMT5 (unknown origin) at 50 and 100 uM preincubated for 15 mins followed by substrate and [3H]-SAM addition for 60 mins measured after further 60 mins flashplate assay | Homo sapiens | 1 | single protein format | Scientific Literature |
The page will load shortly, Thanks for your patience!