| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1804755 | B | Inhibition of DOTL1-mediated methylation of nucleosome at 100 uM after 90 mins by scintillation counting in presence of S-adenosyl-l-[methyl-3H]methionine | 3 | single protein format | Scientific Literature | |||
| 2. | ALA2173352 | B | Inhibition of human recombinant DOT1L using [3H]-SAM as substrate after 30 mins | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 3. | ALA2173380 | B | Inhibition of GST-tagged human DOT1L amino acid 1 to 472 expressed in Escherichia coli BL21 (DE3) using [3H]-SAM as substrate preincubated for 10 mins before substrate addition measured after 30 mins by scintillation counter | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 4. | ALA2175495 | B | Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 20 uM of SAH | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA2175496 | B | Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 10 uM of SAH | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA2175497 | B | Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 5 uM of SAH | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA2175498 | B | Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 2.5 uM of SAH | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA2175605 | B | Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 1 uM of SAH | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 9. | ALA2175606 | B | Binding affinity at human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 10. | ALA2175607 | B | Binding affinity at human recombinant DOT1L catalytic domain amino acid (1 to 472) by isothermal titration calorimetric assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 11. | ALA2175616 | B | Inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472) using [3H]-SAM after 30 mins by scintillation counter | Homo sapiens | 56 | single protein format | Scientific Literature | ||
| 12. | ALA2209116 | B | Inhibition of DOT1L using core histone as substrate preincubated for 10 mins followed by addition of [3H]SAM and incubated for 60 mins | 3 | single protein format | Scientific Literature | |||
| 13. | ALA2330194 | B | Inhibition of DOT1L (unknown origin) using nucleosome as substrate after 1 hr by filter plate assay in presence of [3H]-S-adenosylmethionine | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA2350637 | B | Competitive inhibition of human recombinant DOT1L (1 to 420 amino acid residues) overexpressed in Escherichia coli BL21 (DE3) using [3H]-SAM as substrate assessed as inhibition of nucleosome methylation incubated for 30 mins prior to substrate addition measured after 1 hr by scintillation counting analysis | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 15. | ALA2350638 | B | Ratio of SAH IC50 to compound IC50 for inhibition of human recombinant DOT1L (1 to 420 amino acid residues) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA2350639 | B | Binding affinity to human recombinant DOT1L (1 to 420 amino acid residues) overexpressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature at 50 uM by differential scanning fluorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA2350651 | B | Competitive inhibition of human recombinant DOT1L (1 to 472 amino acid residues) expressed in Escherichia coli BL21 (DE3) using [3H]-SAM assessed as inhibition of nucleosome methylation incubated for 10 mins prior to substrate addition measured after 30 mins by scintillation counting analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 18. | ALA2350652 | B | Competitive inhibition of human recombinant DOT1L (1 to 416 amino acid residues) using [3H]-SAM assessed as inhibition of nucleosome methylation incubated for 30 mins prior to substrate addition measured after 120 mins | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 19. | ALA3090816 | B | Inhibition of DOT1L (unknown origin)-mediated H3K79 methylation by cell based assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 20. | ALA3090819 | B | Ratio of S-adenosyl-L-homocysteine IC50 to compound IC50 for recombinant human DOT1L using adenosine/deazaadenosine as substrate and SAM cofactor | Homo sapiens | 1 | single protein format | Scientific Literature |
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