#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1804755	Binding	Inhibition of DOTL1-mediated methylation of nucleosome at 100 uM after 90 mins by scintillation counting in presence of S-adenosyl-l-[methyl-3H]methionine		3	ALA1799916	single protein format	Scientific Literature
2.	ALA3390901	Binding	Inhibition of recombinant human DOT1L using chicken erythrocyte oligonucleosomes/[3H]-SAM as substrate assessed as incorporation of radioactivity into oligonucleosome preincubated for 30 mins followed by substrate addition measured after 120 mins	Homo sapiens	1	ALA3390834	single protein format	Scientific Literature
3.	ALA3390903	Binding	Inhibition of DOT1L in human MOLM13 cells assessed as downregulation of HOXA9/MEIS1 mRNA expression after 6 days by real-time PCR analysis	Homo sapiens	1	ALA3390834	cell-based format	Scientific Literature
4.	ALA3390904	Binding	Inhibition of DOT1L in human MV4-11 cells assessed as downregulation of HOXA9/MEIS1 mRNA expression after 6 days by real-time PCR analysis	Homo sapiens	1	ALA3390834	cell-based format	Scientific Literature
5.	ALA3378782	Binding	Inhibition of DOT1L (unknown origin) by filter-based assay	Homo sapiens	1	ALA3351294	single protein format	Scientific Literature
6.	ALA4377331	Binding	Inhibition of human DOT1L up to 50 uM using chicken nucleosome as substrate and in presence of [3H]-SAM incubated for 1 hr by filter-based assay	Homo sapiens	1	ALA4376809	single protein format	Scientific Literature
7.	ALA2173352	Binding	Inhibition of human recombinant DOT1L using [3H]-SAM as substrate after 30 mins	Homo sapiens	1	ALA2169811	single protein format	Scientific Literature
8.	ALA2173380	Binding	Inhibition of GST-tagged human DOT1L amino acid 1 to 472 expressed in Escherichia coli BL21 (DE3) using [3H]-SAM as substrate preincubated for 10 mins before substrate addition measured after 30 mins by scintillation counter	Homo sapiens	2	ALA2169811	assay format	Scientific Literature
9.	ALA2175495	Binding	Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 20 uM of SAH	Homo sapiens	1	ALA2169812	single protein format	Scientific Literature
10.	ALA2175496	Binding	Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 10 uM of SAH	Homo sapiens	1	ALA2169812	single protein format	Scientific Literature
11.	ALA2175497	Binding	Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 5 uM of SAH	Homo sapiens	1	ALA2169812	single protein format	Scientific Literature
12.	ALA2175498	Binding	Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 2.5 uM of SAH	Homo sapiens	1	ALA2169812	single protein format	Scientific Literature
13.	ALA2175605	Binding	Competitive inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay in presence of 1 uM of SAH	Homo sapiens	1	ALA2169812	single protein format	Scientific Literature
14.	ALA2175606	Binding	Binding affinity at human recombinant DOT1L catalytic domain amino acid (1 to 472)-nucleosome complex by isothermal titration calorimetric assay	Homo sapiens	3	ALA2169812	single protein format	Scientific Literature
15.	ALA2175607	Binding	Binding affinity at human recombinant DOT1L catalytic domain amino acid (1 to 472) by isothermal titration calorimetric assay	Homo sapiens	3	ALA2169812	single protein format	Scientific Literature
16.	ALA2175616	Binding	Inhibition of human recombinant DOT1L catalytic domain amino acid (1 to 472) using [3H]-SAM after 30 mins by scintillation counter	Homo sapiens	56	ALA2169812	single protein format	Scientific Literature
17.	ALA2209116	Binding	Inhibition of DOT1L using core histone as substrate preincubated for 10 mins followed by addition of [3H]SAM and incubated for 60 mins		3	ALA2202955	single protein format	Scientific Literature
18.	ALA2330194	Binding	Inhibition of DOT1L (unknown origin) using nucleosome as substrate after 1 hr by filter plate assay in presence of [3H]-S-adenosylmethionine	Homo sapiens	1	ALA2321656	single protein format	Scientific Literature
19.	ALA2350637	Binding	Competitive inhibition of human recombinant DOT1L (1 to 420 amino acid residues) overexpressed in Escherichia coli BL21 (DE3) using [3H]-SAM as substrate assessed as inhibition of nucleosome methylation incubated for 30 mins prior to substrate addition measured after 1 hr by scintillation counting analysis	Homo sapiens	3	ALA2346640	assay format	Scientific Literature
20.	ALA2350638	Binding	Ratio of SAH IC50 to compound IC50 for inhibition of human recombinant DOT1L (1 to 420 amino acid residues)	Homo sapiens	1	ALA2346640	single protein format	Scientific Literature