# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3705674 | Binding | Fluorescence Polarization Ligand Binding Assay: The glucocorticoid receptor fluorescence polarization ligand binding (GRFP) assay is used to evaluate direct binding of testing compounds to full-length glucocorticoid (GR) protein. Reagents for this assay are purchased from Invitrogen in a test kit. A fluorescent labeled GR ligand is used as a fluorescent tracer and test compounds compete with the fluorescent tracer for GR binding. The change in polarization value in the presence of test compounds is due to binding of test compounds to GR and is used to determine IC50 and relative binding affinity of test compounds for GR. | 12 | ALA3638885 | single protein format | BindingDB Database | ||
2. | ALA1809407 | Binding | Inhibition of IL-6 at 4 uM | 1 | ALA1806459 | single protein format | Scientific Literature | ||
3. | ALA4035162 | Binding | Inhibition of IL-6 signaling pathway in human HepG2 cells transfected with p-STAT3-Luc assessed as reduction in IL-6 induced STAT3 phosphorylation at 20 ug/ml preincubated for 1 hr followed by IL-6 induction measured after 10 mins by immunoblot analysis | Homo sapiens | 16 | ALA4033664 | cell-based format | Scientific Literature | |
4. | ALA4035163 | Binding | Inhibition of IL-6 signaling pathway in human HepG2 cells transfected with p-STAT3-Luc assessed as reduction in IL-6 induced STAT3 phosphorylation preincubated for 1 hr followed by IL-6 induction measured after 10 mins by immunoblot analysis | Homo sapiens | 3 | ALA4033664 | cell-based format | Scientific Literature | |
5. | ALA4046839 | Binding | Inhibition of IL6 (unknown origin) | Homo sapiens | 1 | ALA4043202 | single protein format | Scientific Literature | |
6. | ALA4882182 | Binding | Fibroblast IL-6 assay | Homo sapiens | 1 | ALA4507277 | cell-based format | Donated Chemical Probes - SGC Frankfurt | |
7. | ALA4885694 | Binding | In vitro inhibition of IL-6 in Lipopolysaccharide-stimulated human whole blood | Homo sapiens | 1 | ALA4630905 | tissue-based format | Donated Chemical Probes - SGC Frankfurt | |
8. | ALA5128168 | Binding | Inhibition of IL-6 (unknown origin) relative to control | Homo sapiens | 1 | ALA5126538 | single protein format | Scientific Literature | |
9. | ALA5231473 | Binding | Inhibition of IL-6 (unknown origin) by ELISA assay | Homo sapiens | 7 | ALA5230116 | single protein format | Scientific Literature |