| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1832596 | B | Inhibition of SETD8 assessed as hydrolysis of S-adenosyl-L-homocysteine after 2 mins by SAHH-coupled fluorescence assay | 1 | single protein format | Scientific Literature | |||
| 2. | ALA1217461 | B | Activity at methyl transferase activity SET8/preSet7 by enzyme coupled S-adenocylehomocystein detection assay | 3 | single protein format | Scientific Literature | |||
| 3. | ALA2330200 | B | Inhibition of SETD8 (unknown origin) using histone H4 (1 to 24) as substrate after 1 hr by scintillation proximity assay in presence of [3H]-S-adenosylmethionine | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA2350647 | B | Inhibition of SETD8 (unknown origin) using [3H]SAM assessed as inhibition of biotinylated-H3 (1 to 25 amino acid residues) methylation at 0.1 to 100 uM after 1 hr by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA3111895 | B | Inhibition of SETD8 (unknown origin)-mediated incorporation of methyl group from [3H]-SAM into peptide substrate up to 50 uM by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA3378195 | B | Inhibition of SETD8 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA3378196 | B | Inhibition of SETD8 (unknown origin) catalytic domain expressed in Escherichia coli using biotinylated H4 (1-24) peptide substrate after 1 hr by scintillation proximity assay | Homo sapiens | 24 | assay format | Scientific Literature | ||
| 8. | ALA3378197 | B | Inhibition of SETD8 (unknown origin) catalytic domain expressed in Escherichia coli by ITC assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA3378198 | B | Inhibition of SETD8 (unknown origin) catalytic domain expressed in Escherichia coli by SPR assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA3378199 | B | Competitive inhibition of SETD8 (unknown origin) catalytic domain expressed in Escherichia coli using varying levels of biotinylated H4 (1-24) peptide substrate by scintillation proximity assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA3378200 | B | Non-competitive inhibition of SETD8 (unknown origin) catalytic domain expressed in Escherichia coli using biotinylated H4 (1-24) peptide substrate and varying concentrations of SAM by scintillation proximity assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA3378201 | B | Displacement of FITC-labeled H4K20me (1-24) from SETD8 (unknown origin) catalytic domain expressed in Escherichia coli by fluorescence polarization assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 13. | ALA3378784 | B | Inhibition of methyltransferase activity of SETD8 (unknown origin) catalytic domain expressed in Escherichia coli assessed as decrease in methylation of fluorescein labeled TW21 peptide incubated for 10 mins by microfluidic capillary electrophoresis method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA3420963 | B | Inhibition of SETD8 (unknown origin) using H4 (1 to 24) as substrate assessed as incorporation of [3H]-methyl group from [3H-Me]-SAM to peptide substrate after 1 hr by scintillation proximity assay in presence of [3H]-SAM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA3420964 | B | Competitive inhibition of SETD8 (unknown origin) using H4 (1 to 24) as substrate assessed as incorporation of [3H]-methyl group from [3H-Me]-SAM to peptide substrate after 1 hr by Lineweaver-Burk plot analysis in presence of SAM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA3420965 | B | Inhibition of N-terminal His6-tagged human SETD8 (191 to 352) assessed as incorporation of [3H]-methyl group from [3H-Me]-SAM to biotinylated H4K20 peptide substrate preincubated for 10 mins followed by substrate addition by liquid scintillation counting analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 17. | ALA3592291 | B | Inhibition of SETD8 (unknown origin) assessed as incorporation of tritium labeled methyl group from [3H]-SAM to substrate at 1 uM after 1 hr by scintillation proximity assay relative to control | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA3626067 | B | Inhibition of human SETD8 catalytic domain expressed in Escherichia coli assessed as reduction in transfer of tritiated methyl group from [3H]SAM to biotinylated H4 (1 to 24) peptide substrate by scintillation proximity assay | Homo sapiens | 43 | assay format | Scientific Literature | ||
| 19. | ALA3626821 | B | Inhibition of SETD8 (unknown origin) at 100 uM preincubated for 15 mins measured after 120 mins by microbeta counting analysis in presence of [3H]-SAM relative to control | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 20. | ALA3784096 | B | Inhibition of human SETD8 at 10 and 50 uM | Homo sapiens | 1 | single protein format | Scientific Literature |
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