# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1832852 | B | Binding affinity to second bromodomain of BRD3 assessed as change in melting temperature at 100 uM by differential scanning fluorimetry | 9 | single protein format | Scientific Literature | |||
2. | ALA1832853 | B | Binding affinity to first bromodomain of BRD3 assessed as change in melting temperature at 100 uM by differential scanning fluorimetry | 9 | single protein format | Scientific Literature | |||
3. | ALA1953101 | B | Binding affinity to His-6 tagged BRD3 expressed in Escherichia coli at 100 uM after 60 mins by fluorescence anisotropic analysis | 4 | assay format | Scientific Literature | |||
4. | ALA1953104 | B | Inhibition of His-6 tagged BRD3-RD12 precoupled with biotinylated tetra-acetylated histone H4 expressed in Escherichia coli assessed as protein-protein interaction at 50 uM after 1 hr by TR-FRET assay | 4 | assay format | Scientific Literature | |||
5. | ALA1953107 | B | Inhibition of His-6 tagged BRD3 expressed in Escherichia coli after 60 mins by fluorescence anisotropic analysis | 2 | assay format | Scientific Literature | |||
6. | ALA1953110 | B | Inhibition of His-6 tagged BRD3-RD12 interaction precoupled with biotinylated tetra-acetylated histone H4 expressed in Escherichia coli after 1 hr by TR-FRET assay | 1 | assay format | Scientific Literature | |||
7. | ALA1958929 | B | Binding affinity to BRD3-BD1 assessed as change in melting temperature at 100 uM by protein stability shift assay | 12 | single protein format | Scientific Literature | |||
8. | ALA1958938 | B | Binding affinity to BRD3-BD1 assessed as change in melting temperature at 10 uM by protein stability shift assay | 6 | single protein format | Scientific Literature | |||
9. | ALA1959067 | B | Binding affinity to BRD3-BD2 assessed as change in melting temperature at 100 uM by protein stability shift assay | 12 | single protein format | Scientific Literature | |||
10. | ALA1959070 | B | Binding affinity to BRD3-BD2 assessed as change in melting temperature at 10 uM by protein stability shift assay | 6 | single protein format | Scientific Literature | |||
11. | ALA1958559 | B | Binding affinity to BRD3-BD2 by isothermal titration calorimetry | 1 | single protein format | Scientific Literature | |||
12. | ALA1958560 | B | Binding affinity to BRD3-BD1 by isothermal titration calorimetry | 1 | single protein format | Scientific Literature | |||
13. | ALA1958565 | B | Binding affinity to BRD3-BD1,2 by isothermal titration calorimetry | 2 | single protein format | Scientific Literature | |||
14. | ALA1958572 | B | Displacement of acetylated histone peptide from BRD3-BD1,2 by FRET assay | 2 | single protein format | Scientific Literature | |||
15. | ALA2156111 | B | Binding affinity to N-terminal portion of human Brd3 containing bromodomain BD12 expressed in Escherichia coli by isothermal titration calorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
16. | ALA2156118 | B | Displacement of tetra-acetylated H4 peptide from human Brd3 bromodomain BD12 after 1 hr by FRET analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
17. | ALA2182551 | B | Inhibition of His6FLAG-tagged BRD3-BD12 expressed in Escherichia coli assessed as change in melting temperature at 100 uM by thermal shift assay | 2 | assay format | Scientific Literature | |||
18. | ALA2182556 | B | Binding affinity to His6-tagged BRD3 expressed in Escherichia coli by isothermal colorimetric analysis | 2 | assay format | Scientific Literature | |||
19. | ALA2182559 | B | Inhibition of His6-tagged BRD3 expressed in Escherichia coli assessed as inhibition of binding to SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHGSGSK-biotin after 1 hr by TR-FRET assay | 44 | assay format | Scientific Literature | |||
20. | ALA2182563 | B | Inhibition of His6-tagged BRD3 expressed in Escherichia coli at 200 uM after 60 mins by fluorescence anisotropy assay | 1 | assay format | Scientific Literature |